The gene for the extracellular alpha antigen of Mycobacterium bovis BCG was cloned by using a single probe restricted to G or C in third position. This technique should have great potential isolation mycobacterial genes. analysis revealed that encoded 323 amino acid residues, including 40 acids signal peptide followed 283 mature protein. is first report on structure peptide. promoter-like sequence and ribosome-binding site were observed upstream open reading frame. In coding region, position...

The gene for immunogenic protein MPB64 found in culture filtrates of only Mycobacterium tuberculosis and some strains bovis BCG was cloned by using a single-probe method sequenced. analysis revealed that the structural consisted 618 base pairs, its deduced molecular weight 22,400. Twenty-two amino acids putative signal peptide 205 were observed. In coding region, third letter codon showed biased high G+C content (78.5%). expressed Escherichia coli an E. expression vector. product migration...

We have previously demonstrated that conjugation of small molecule ligands to interfering RNAs (siRNAs) and anti-microRNAs results in functional siRNAs antagomirs vivo. Here we report on the development an efficient chemical strategy make oligoribonucleotide-ligand conjugates using copper-catalyzed azide-alkyne cycloaddition (CuAAC) or click reaction. Three reaction approaches were evaluated for their feasibility suitability high-throughput synthesis: CuAAC at monomer level prior...

A resistance (R) factor- containing strain of Escherichia coli which is known to inactivate streptomycin by adenylylation has been shown be spectinomycin resistant. An osmotic shockate this catalyzes the formation biologically inactive adenylate, in adenylyl residue probably attached a d - threo methylamino alcohol moiety spectinomycin. Both and adenylylating activities show same temperature inactivation profile, both are present protein fraction purified for inactivating enzyme. Mutants...

Resin composites with antibacterial activity may be useful to decrease the frequency of secondary caries around restorations. The purposes this study were investigate light-activated resin incorporating one three silver-containing materials and evaluate their long-term inhibitory effect against Streptococcus mutans types materials, Novaron (N), Amenitop (AM), AIS, incorporated into TEGDMA-UDMA-based composites, activities, mechanical properties release silver ions examined. Minimum...

Lysophosphatidic acid (LPA) is a serum-derived phospholipid that induces variety of biological responses in various cells via heterotrimeric G protein-coupled receptors (GPCRs) including LPA1, LPA2, and LPA3. LPA-induced cytoskeletal changes are mediated by Rho family small GTPases, such as RhoA, Rac1, Cdc42. One these may be activated Galpha(12/13)-linked Rho-specific guanine nucleotide exchange factors (RhoGEFs) under LPA stimulation although the detailed mechanisms poorly understood....

In order to develop recombinant Mycobacterium bovis BCG into a useful multivaccine vehicle, we established foreign antigen secretion system in mycobacteria which an extracellular alpha of kansasii was utilized as carrier. By using this system, B-cell epitope (Glu-12-Leu-Asp-Arg-Trp-Glu-Lys-Ile-19) human immunodeficiency virus type 1 p17gag, identified by fusion protein-based method, has been successfully obtained from along with the antigen. This is first report expression and viral BCG. It...

The merozoite surface protein 1 (MSP1) has emerged as a leading malaria vaccine candidate at the erythrocytic stage. Recombinant bacillus Calmette-Guérin (rBCG), which expressed COOH-terminal 15-kD fragment of MSP1 Plasmodium yoelii (MSP1-15) fusion with secretory Mycobacterium kansasii, was constructed. Immunization mice this rBCG induced higher degree protection against blood-stage parasite infection than recombinant MSP1-15 in RIBI adjuvant (RIBI ImmunoChem Research, Inc., Hamilton, MT)...

The number of rRNA genes in Mycobacterium bovis BCG was examined by Southern hybridization end-labeled 5S, 16S, and 23S rRNAs with BamHI, PstI, SalI digests M. DNA. Each RNA probe gave only one radioactive band three kinds DNA digest. These results suggest that chromosomes may carry a minimum set genes. Hybridization randomly labeled SalI, BglII, PvuII from the same organism supported these conclusions. 6.4-kilobase-pair fragment containing entire structural for both 16S cloned into pBR322....

The gene for the extracellular alpha antigen of Mycobacterium kansasii was cloned by using alpha-antigen fragments M. bovis BCG as probes. Gene analysis revealed that this encodes 325 amino acid residues, including 40 acids signal peptide, followed 285 mature protein. A comparison nucleotide sequences genes isolated from these two mycobacterial species showed levels DNA and homology were 84.8 89.1%, respectively. hydropathy profiles also compared, highly changed hydrophilic regions observed,...

The complete nucleotide sequence of the 16S rRNA gene Mycobacterium bovis BCG was determined. Its coding region estimated to be 1,536 base pairs long. in M. has homologies 75 and 89% with those Escherichia coli Streptomyces lividans, respectively.

Virus-specific T-cell responses can limit immunodeficiency virus type 1 (HIV-1) transmission and prevent disease progression so could serve as the basis for an affordable, safe, effective vaccine in humans. To assess their potential a vaccine, we used Mycobacterium bovis bacillus Calmette-Guérin (BCG)-Tokyo replication-deficient vaccinia strain (DIs) vectors to express full-length gag from simian viruses (SIVs) (rBCG-SIVgag rDIsSIVgag). Cynomolgus macaques were vaccinated with either...

The extracellular protein MPB70 is a heat-stable immunogenic which was found in the culture filtrate of Mycobacterium bovis BCG Japanese. We determined complete nt and aa sequences correlated with previously reported data. N-terminal sequence revealed that signal peptide (SP) consisted 30 mature had 163 molecular weight 16,305. SP displayed characteristic feature an Ala-rich property would be efficient function.

The mechanism of resistance Mycobacterium intracellulare strain 103 and other clinical isolates to a variety drugs including aminoglycoside peptide antibiotics was investigated. Enzymatic inactivation could not be demonstrated. Ribosomes the were found sensitive antibiotics. levels decreased dramatically when culture medium changed from Dubos agar Tween 80-containing agar. These results suggest that permeability barrier is reason for naturally occurring in M. intracellulare.

Protection against Mycobacterium tuberculosis not only depends on CD4+ T helper type 1 (Th1) cells but, also, CD8+ cells. Interleukin (IL)-15 has an important function in the maintenance of memory In present study, we examined efficacy recombinant bovis bacille Calmette-Guérin (rBCG) secreting fusion protein antigen (Ag) 85B murine IL-15 (rBCG-Ag85B-IL15) providing protection M. infection. The levels major histocompatibility (MHC) class Ib (H2-M3)-binding TB2- or MHC Ia (H-2Db)-binding...

Viomycin-resistant strains were isolated from Mycobacterium smegmatis . Ribosomes and tested for drug resistance in subcellular systems containing poly(U) as messenger ribonucleic acid. Resistance to viomycin these was due altered ribosomes. Further analysis showed that of two mutants with low level (20 μg/ml) 30 S ribosomal subunits. Another mutant highly resistant (1 mg/ml), however, had 50

Aminoglycoside antibiotics specifically affect protein synthesis in bacteria, and while the mechanism by which is stopped has not yet been completely elucidated (several steps synthetic process may be susceptible), fact that mutational alteration of ribosomes primary resistance proved great utility. Resistance to aminoglycoside bacteria shown produce altered 30 S ribosomal subunits, these mutations have allowed genetic mapping loci responsible for proteins this subunit, serve also as...

The binding motifs of the immunodominant antigen (Ag) α-Ag (Ag 85 complex B) Mycobacterium kansasii for human fibronectin were examined using digested fragments. We defined two fibronectin-binding epitopes on 27 amino acids from 84 to 110 and 20 211 230. almost conserved in closely related Ag other mycobacteria species. Inhibition intact molecules was observed with peptide-(84–110), but not peptide-(211–230). Peptide-(84–110) could also inhibit all components Bacillus Calmette-Guérin 85A,...

This study was to establish a more effective anti-cancer immunomodulating agent by constructing recombinant (r) Bacillus Calmette-Guérin (BCG) secreting alpha-antigen (alpha-Ag) fused murine (m) interleukin (IL)-2, and its biological activity on cell-mediated cytotoxicity against bladder cancer cell, MBT-2, in vitro.pSO246 plasmid vector ligated with mIL-2 gene introduced into BCG electroporation. Thioglycollate-elicited peritoneal exudate cells (PEC) were stimulated vitro parental or rBCG...

The structure of the complex formed by naphthyridine carbamate dimer (NCD) binding to CGG repeat sequences in DNA, associated with fragile X syndrome, has been elucidated using 15 N-labeled NCD and 1 H– N HSQC.