A5011987713- Orthopedic Infections and Treatments
- Orthopaedic implants and arthroplasty
- Total Knee Arthroplasty Outcomes
- Connective tissue disorders research
- Cell Adhesion Molecules Research
- Infectious Diseases and Tuberculosis
- Venous Thromboembolism Diagnosis and Management
- Surgical site infection prevention
- Religion, Society, and Development
- Anesthesia and Pain Management
- Tendon Structure and Treatment
- Bone and Dental Protein Studies
- HER2/EGFR in Cancer Research
- Bacterial biofilms and quorum sensing
- Knee injuries and reconstruction techniques
- Antimicrobial Resistance in Staphylococcus
- Atrial Fibrillation Management and Outcomes
- Monoclonal and Polyclonal Antibodies Research
- Cardiac, Anesthesia and Surgical Outcomes
- Opioid Use Disorder Treatment
- Infective Endocarditis Diagnosis and Management
- Dental Anxiety and Anesthesia Techniques
- Musculoskeletal Disorders and Rehabilitation
- Theological Perspectives and Practices
- Bone fractures and treatments
Thomas Jefferson University Hospital
2011-2023
Rothman Institute
2013-2023
Thomas Jefferson University
1996-2023
Rothman Orthopaedics
2019-2021
John Wiley & Sons (United States)
2019
People's Liberation Army No. 150 Hospital
2009-2011
University College London
2009-2011
Royal National Orthopaedic Hospital
2009-2011
Jefferson Institute
1996-1998
Union Theological Seminary
1977-1997
ABSTRACT Biofilm‐associated implant‐related bone and joint infections are clinically important due to the extensive morbidity, cost of care socioeconomic burden that they cause. Research in field biofilms has expanded past two decades, however, there is still an immense knowledge gap related many clinical challenges these biofilm‐associated infections. This subject was assigned Biofilm Workgroup during second International Consensus Meeting on Musculoskeletal Infection held Philadelphia USA...
Bone morphogenic protein-1 (BMP-1) was originally identified as one of several BMPs that induced new bone formation when implanted into ectopic sites in rodents. BMP-1, however, differed from other it its structure not similar to transforming growth factor beta. Instead, had a large domain homologous metalloendopeptidase isolated crayfish, an epidermal growth-factor-like domain, and three regions internal sequence homology referred CUB domains. Therefore, BMP-1 member the "astacin families"...
Abstract Antibiotic‐loaded bone cement (ALBC) is broadly used to treat orthopaedic infections based on the rationale that high‐dose local delivery essential eradicate biofilm‐associated bacteria. However, ALBC formulations are empirically drug susceptibility from routine laboratory testing, which known have limited clinical relevance for biofilms. There also dosing concerns with nonstandardized, surgeon‐directed, hand‐mixed formulations, unknown release kinetics. On basis of our knowledge in...
Prophylaxis for pulmonary embolism (PE) after total joint arthroplasty (TJA) presents the clinical dilemma of balancing risk postoperative thrombotic and anticoagulation-related complications such as bleeding, hematoma formation, infection. Risk stratification patients undergoing TJA is needed to tailor prophylaxis based on bleeding risk.The purpose this study was identify preoperative comorbidities that were associated with an increased symptomatic PE in a large group who had TJAs treated...
Post-traumatic joint contracture is a frequent orthopaedic complication that limits the movement of injured joints, thereby severely impairing affected patients. Non-surgical and surgical treatments for often fail to improve range motion. In this study, we tested hypothesis limiting formation collagen-rich tissue in capsules joints would reduce consequences fibrotic response mobility. We targeted collagen fibrils, main component deposits formed within tissues by employing relevant rabbit...
Introduction: Opioids remain the most prescribed medication after total hip arthroplasty (THA) despite potential for abuse and adverse effects. Given high rates of opioid effects, reporting controlled substances is now mandatory in many statewide databases. This study aimed to use a database analyze prescription patterns postoperative THA patients identify independent risk factors those who need second and/or require prolonged (>6 months). Methods: We retrospectively reviewed consecutive...
A cDNA cassette system was used to synthesize recombinant versions of procollagen II in which one the four blocks 234 amino acids that define a repeating D periods collagen triple helix were deleted. All proteins helical and all underwent helix-to-coil transition between 25 42 degreesC as assayed by circular dichroism. However, details melting curves varied. The lacking D1 period unfolded 3 lower than full-length molecule. With D4 period, first 25% unfolding occurred at temperature molecule,...
ABSTRACT Post‐traumatic joint contracture is a debilitating consequence of trauma or surgical procedures. It associated with fibrosis that develops regardless the nature initial and results from complex biological processes inflammation cell activation. These accelerate production structural elements extracellular matrix, particularly collagen fibrils. Although increased collagenous proteins has been demonstrated in tissues contracted joints, researchers have not yet determined protein...
Journal of Orthopaedic ResearchVolume 32, Issue S1 p. S31-S59 Research ArticleFree Access Perioperative Antibiotics First published: 24 January 2014 https://doi.org/10.1002/jor.22549Citations: 15AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text full-text accessPlease review our Terms and Conditions Use check box below share version article.I have read accept the Wiley Online Library UseShareable LinkUse link a this...
Inhibition of the extracellular process collagen fibril formation represents a new approach to limiting posttraumatic or postsurgical localized fibrosis. It has been demonstrated that employing monoclonal antibody targets C-terminal telopeptide α2 chain I blocks critical I–collagen interaction, thereby reducing amount deposits in vitro and animal models. Here, we developed chimeric variant prototypic inhibitory mouse origin. The structure this novel was analyzed by biochemical biophysical...
Posttraumatic fibrotic scarring is a significant medical problem that alters the proper functioning of injured tissues. Current methods to reduce posttraumatic fibrosis rely on anti-inflammatory and anti-proliferative agents with broad intracellular targets. As result, their use not fully effective may cause unwanted side effects. Our group previously demonstrated extracellular collagen fibrillogenesis valid specific target collagen-rich scar buildup. previous studies showed rationally...
Collagens are a key element in the architecture of all organs and tissues. These proteins not only build extracellular scaffolds that define mechanical properties tissues, but also play an important role as cell signaling molecules. Certain characteristics collagens enable them to fulfill specific functions, including their triple-helical structure ability self-assemble into complex structures. Their unique allow serve material for tissue repair engineering, drug delivery vehicle, and, form...
This study focuses on the single-chain fragment variable (scFv) variant of original IgA-type antibody, recognizing α2 C-terminal telopeptide (α2Ct) human collagen I, designed to inhibit post-traumatic localized fibrosis via blocking formation collagen-rich deposits. We have demonstrated that scFv construct expressed in yeast cells was able fold into an immunoglobulin-like conformation, but it prone forming soluble aggregates. Functional assays, however, indicate specifically binds α2Ct...
Prosthetic joint infections are difficult to diagnose and treat due biofilm formation by the causative pathogens. Pathogen identification relies on microbial culture that requires days weeks, in case of chronic infections, lacks sensitivity. Diagnosis infection is often delayed past point effective treatment such only removal implant curative. Early diagnosis an based antibody detection might lead less invasive, early interventions.