A5101983199- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Nitric Oxide and Endothelin Effects
- Atherosclerosis and Cardiovascular Diseases
- Sulfur Compounds in Biology
- RNA modifications and cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA Research and Splicing
- Molecular Sensors and Ion Detection
- Kruppel-like factors research
- Adenosine and Purinergic Signaling
- Extracellular vesicles in disease
- Nuclear Receptors and Signaling
- Cancer, Hypoxia, and Metabolism
- Metabolism, Diabetes, and Cancer
- Cardiovascular Function and Risk Factors
- Genetic and phenotypic traits in livestock
- Eicosanoids and Hypertension Pharmacology
- RNA Interference and Gene Delivery
- Neuroinflammation and Neurodegeneration Mechanisms
- NF-κB Signaling Pathways
- Sunflower and Safflower Cultivation
- Plant Pathogens and Fungal Diseases
- Immune cells in cancer
Affiliated Hospital of Southwest Medical University
2022-2025
Southwest Medical University
2010-2025
Shanxi Agricultural University
2023-2024
Rush University Medical Center
2013-2024
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2015-2024
Wenzhou Medical University
2015-2024
Hunan University of Arts and Science
2021-2024
Shandong Academy of Forestry
2023
Binzhou Medical University
2018-2022
Tianjin Stomatological Hospital
2014-2022
MicroRNAs (miRNAs) are a recently discovered class of endogenous, small, noncoding RNAs that regulate about 30% the encoding genes human genome. However, role miRNAs in vascular disease is currently completely unknown. Using microarray analysis, we demonstrated for first time aberrantly expressed walls after balloon injury. The were further confirmed by Northern blot and quantitative real-time polymerase chain reaction. Modulating an overexpressed miRNA, miR-21, via antisense-mediated...
Phenotypic modulation of vascular smooth muscle cells (VSMCs) plays a critical role in the pathogenesis variety proliferative diseases. Recently, we have found that microRNA (miRNA) miR-145 is most abundant miRNA normal walls and freshly isolated VSMCs; however, VSMC phenotypic diseases currently unknown. Here find selectively expressed VSMCs wall its expression significantly downregulated with neointimal lesion formation cultured dedifferentiated VSMCs. More importantly, both rat vitro...
MicroRNAs (miRNAs) comprise a novel class of endogenous, small, noncoding RNAs that negatively regulate gene expression. Functionally, an individual miRNA is as important transcription factor because it able to the expression its multiple target genes. Recently, miR-221 and miR-222 have been found play critical role in cancer cell proliferation. However, their roles vascular smooth muscle (VSMC) biology are currently unknown. In present study, time course changes cellular distribution were...
miRNAs (microRNAs) participate in many diseases including cardiovascular disease. In contrast with our original hypothesis, exist circulating blood and are relatively stable due to binding other materials. The aim of the present translational study is establish a method determining absolute amount an miRNA determine potential applications cell-free miR-1 (microRNA-1) AMI (acute myocardial infarction). results revealed that most abundant heart also heart- muscle-specific miRNA. cardiac cell...
Vascular cells, particularly endothelial adopt aerobic glycolysis to generate energy support cellular functions. The effect of on angiogenesis remains unclear. 6-Phosphofructo-2-kinase/fructose-2, 6-bisphosphatase, isoform 3 (PFKFB3) is a critical enzyme for glycolysis. By blocking or deleting PFKFB3 in we investigated the influence both vitro and vivo.Under hypoxic conditions after treatment with angiogenic factors, was upregulated vivo. knockdown overexpression suppressed accelerated...
Sociosexual preference is critical for reproduction and survival. However, neural mechanisms encoding social decisions on sex remain unclear. In this study, we show that both male female mice exhibit but shift to when facing survival threats; their mediated by the dimorphic changes in excitability of ventral tegmental area dopaminergic (VTA DA ) neurons. males, VTA projections nucleus accumbens (NAc) mediate preference, those medial preoptic preference. females, firing-pattern (phasic-like...
Long noncoding RNAs (lncRNAs) play important roles in the development of vascular diseases.However, effect lncRNA NORAD on atherosclerosis remains unknown.This study aimed to investigate endothelial cell injury and atherosclerosis.Ox-LDL-treated human umbilical vein cells (HUVECs) high-fat-diet (HFD)-fed ApoE -/-mice were used as vitro vivo models.Results showed that NORAD-knockdown induced cycle arrest G0/G1 phase, aggravated ox-LDL-induced viability reduction, apoptosis, senescence along...
Activation of Notch signaling by Jagged-1 (Jag-1) in vascular smooth muscle cells (VSMC) promotes a differentiated phenotype characterized increased expression contractile proteins. Recent studies show that microRNAs (miR)-143/145 regulates VSMC phenotype. The serum response factor (SRF)/myocardin complex binds to CArG sequences activate miR-143/145 transcription, but no other regulators are known VSMC. Using miR arrays, we found induced following constitutively active Notch1 intracellular...
Abstract Increased aerobic glycolysis in endothelial cells of atheroprone areas blood vessels has been hypothesized to drive increased inflammation and lesion burden but direct links remain be established. Here we show that exposed disturbed flow vivo vitro exhibit levels protein kinase AMP-activated (PRKA)/AMP-activated kinases (AMPKs). Selective deletion Prkaa1 , coding for catalytic subunit alpha1, reduces glycolysis, compromises cell proliferation, accelerates the formation...
Abstract Background Recent studies have shown that circular RNA (circRNA) is rich in microRNA (miRNA) binding sites. We previously demonstrated the antidepressant effect of ketamine related to abnormal expression various miRNAs brain. This study determined profile circRNAs hippocampus rats treated with ketamine. Methods The aberrantly expressed rat after injection were analyzed by microarray chip, and we further validated these quantitative reverse-transcription PCR (qRT-PCR). target genes...
Background This study aimed at investigating whether NLRP 3 (the Nod like receptor family, pyrin domain-containing protein) inflammasome activation induced HMGB 1 (high mobility group box-1 secretion and foam cell formation in human vascular smooth muscle cells ( VSMC s) atherosclerosis ApoE-/- mice. Methods Results s or mice were treated with lipopolysaccharides LPS ) and/or ATP high-fat diet to induce activation. distribution characterized. Liver X α -binding cassette transporter...
Previous studies reported that miR‐146a was involved in small intestine ischemia‐reperfusion (I/R) injury, but the mechanism is largely vague. Here, we aimed to identify change of patients with mesenteric ischemia and explore potential regulatory epithelial cells survival under I/R injury. The plasma 20 25 controls collected examine expression by qPCR. Rat intestinal (IEC‐6) 24 male Sprague–Dawley rats were included build model vitro vivo. qPCR results showed decreased both IEC‐6 rat tissues...