A5049962869- Malaria Research and Control
- Invertebrate Immune Response Mechanisms
- Mosquito-borne diseases and control
- Blood Coagulation and Thrombosis Mechanisms
- Toxoplasma gondii Research Studies
- Sulfur Compounds in Biology
- Insect Resistance and Genetics
- Synthesis and Characterization of Heterocyclic Compounds
- Atrial Fibrillation Management and Outcomes
- HIV Research and Treatment
- Venomous Animal Envenomation and Studies
- Pancreatic function and diabetes
- Selenium in Biological Systems
- Glycosylation and Glycoproteins Research
- Trace Elements in Health
- Vitamin K Research Studies
- Natural Language Processing Techniques
- Redox biology and oxidative stress
- Studies on Chitinases and Chitosanases
- Moringa oleifera research and applications
- vaccines and immunoinformatics approaches
- Heat shock proteins research
- Calpain Protease Function and Regulation
- COVID-19 diagnosis using AI
- Click Chemistry and Applications
Western Connecticut State University
2013-2022
Scripps Research Institute
2008-2021
Clinica Universidad de Navarra
2013
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2013
RWTH Aachen University
2013
Justus-Liebig-Universität Gießen
2013
University of California, San Diego
2003-2010
University of Montana
2010
Marymount Manhattan College
2005
McMaster University
2005
Although the genomes of many most important human and animal pathogens have now been sequenced, our understanding actual proteins expressed by these how well they predict protein sequence expression is still deficient. We used three complementary approaches (two-dimensional electrophoresis, gel-liquid chromatography linked tandem mass spectrometry MudPIT) to analyze proteome Toxoplasma gondii, a parasite medical veterinary significance, developed public repository for data within ToxoDB,...
Abstract We report the proteomes of four life‐cycle stages Apicomplexan parasite Eimeria tenella. A total 1868 proteins were identified, with 630, 699, 845 and 1532 found in early oocysts (unsporulated), late (sporulated), sporozoites second‐generation merozoites, respectively. multidimensional protein identification technology shotgun approach identified 812 sporozoites, 1528 merozoites all oocyst proteins, whereas 2‐D gel proteomics 230 98 merozoite proteins. Comparing invasive stages, we...
Apicomplexan parasites depend on the invasion of host cells for survival and proliferation. Calcium-dependent signaling pathways appear to be essential micronemal release gliding motility, yet target activated kinases remains largely unknown. We have characterized calcium-dependent phosphorylation events during Toxoplasma cell invasion. Stimulation live tachyzoites with Ca2+-mobilizing drugs leads numerous parasite proteins, as shown by differential 2-DE display 32[P]-labeled protein...
Coevolution of the malarial parasite and its human host has resulted in a complex network interactions contributing to homeodynamics host-parasite unit. As rapidly growing multiplying organism, Plasmodium falciparum depends on an adequate antioxidant defense system that is efficient despite absence genuine catalase glutathione peroxidase. Using different experimental approaches, we demonstrate P. imports redox-active protein peroxiredoxin 2 (hPrx-2, hTPx1) into cytosol. shown by confocal...
Secretion of microneme proteins is essential to Plasmodium invasion but the molecular composition these secretory organelles remains poorly defined. Here, we describe first proteome. Purification micronemes by subcellular fractionation from cultured ookinetes was confirmed enrichment known micronemal and electron microscopy. Quantitation micrographs showed >14-fold compared intact ookinete, such that comprised 85% identifiable in fraction. Gel LC-MS/MS most abundant protein constituents...
Gametogenesis and fertilization play crucial roles in malaria transmission. While male gametes are thought to be amongst the simplest eukaryotic cells proven targets of transmission blocking immunity, little is known about their molecular organization. For example, pathway energy metabolism that power motility, a feature facilitates gamete encounter fertilization, unknown.Plasmodium berghei microgametes were purified analysed by whole-cell proteomic analysis for first time. Data available...
Proteome studies contribute markedly to our understanding of parasite biology, host-parasite interactions, and mechanisms drug action. For most antimalarial drugs neither mode action nor resistance development are fully elucidated although this would be important prerequisites for successfully developing urgently required novel antimalarials. Here, we establish a large-scale quantitative proteomic approach examine protein expression changes in trophozoite stages the malarial Plasmodium...
Abstract In eukaryotic cells, the ubiquitin-proteasome system as a key regulator of protein quality control is an excellent drug target. We therefore aimed to analyze 26S proteasome complex in malaria parasite Plasmodium falciparum , which still threatens almost half world’s population. First, we established affinity purification protocol allowing for isolation functional complexes from parasite. Subunit composition and component stoichiometry were studied physiologic interacting partners...
Clusters of complement-type ligand binding repeats in the LDL receptor family are thought to mediate interactions between these receptors and their various ligands. Apolipoprotein E, a key for cholesterol homeostasis, has been shown interact with LDLR, LRP, VLDLR, through clusters. LDLR VLDLR each contain single repeat cluster, whereas LRP contains three large clusters repeats, functions. We show that within sLRP3 three-repeat subcluster CR16-18 recapitulated isolated portion ApoE (residues...
SUMMARY Here we discuss proteomic analyses of whole cell preparations the mosquito stages malaria parasite development (i.e. gametocytes, microgamete, ookinete, oocyst and sporozoite) Plasmodium berghei. We also include critiques proteomes two fractions from purified namely micronemes surface. Whereas summarise key biological interpretations data, try to identify methodological constraints have met, only some which were able resolve. Recognising need translate potential current genome...
Due to its life in different hosts and environments, the human malaria parasite Plasmodium falciparum is exposed oxidative nitrosative challenges. Nitric oxide (NO) NO-derived reactive nitrogen species can constitute stress play a major role NO-related signaling. However, mode of action NO targets P. have hardly been characterized. Protein S-nitrosylation (SNO), posttranslational modification protein cysteine thiols, has emerged as principal mechanism by which exerts diverse biological...
Oxidation of Met 388, one the three linker residues connecting fourth and fifth EGF-like domains thrombomodulin (TM), is deleterious for TM activity. An NMR structure smallest active fragment (TMEGF45) a crystal larger (TMEGF456) bound to thrombin both show that 388 packed into domain. Using multidimensional NMR, we have solved TMEGF45 in which oxidized (TMEGF45ox) mutated Leu (TMEGF45ML). Comparison structures shows domain has somewhat different depending on residue at position several...
Aims: The malaria parasite Plasmodium falciparum possesses four unique selenoproteins (PfSel1–PfSel4) which are likely to represent important components of the redox-regulatory network this infectious agent. So far these proteins have only been characterized in silico. aim present study was gain further insight into structural, biochemical, and functional properties P. selenoproteins. Results: Using 75Se labeling cell culture, presence could be verified for first time. Bioinformatic analyses...
Thrombomodulin (TM) forms a 1:1 complex with thrombin. Whereas thrombin alone cleaves fibrinogen to make the fibrin clot, thrombin−TM protein C initiate anticoagulant pathway. The fourth and fifth EGF-like domains of TM together form minimal fragment cofactor activity. A short linker connects TM, Met 388 in middle interacts both domains. Several different structures TMEGF45 variants are now available, these show that mutation alters structure domain, as well connectivity two To probe this...
Malaria parasites are transmitted by Anopheles mosquitoes. During its life cycle in the mosquito vector Plasmodium ookinete escapes proteolytic milieu of post-blood meal midgut traversing wall. This process requires penetration chitin-containing peritrophic matrix lining epithelium, which depends part on ookinete-secreted chitinases. falciparum ookinetes have one chitinase (PfCHT1), whereas avian-infecting parasite, P. gallinaceum , two, a long and short form, PgCHT1 PgCHT2, respectively....
The ongoing coronavirus disease 2019 (COVID-19) pandemic requires population-wide surveillance testing to test for the presence of severe acute respiratory syndrome 2 (SARS-CoV-2) virus.1 Quantitative reverse-transcription polymerase chain reaction (RT-qPCR) using nasopharyngeal samples has been used as gold-standard detection, but it trained personnel, sterilized swabs, RNA extraction, and causes discomfort in patient being tested.2 As alternative upper tract such saliva have proven be...