A5062660706- Nitric Oxide and Endothelin Effects
- Eicosanoids and Hypertension Pharmacology
- Inflammatory mediators and NSAID effects
- Renin-Angiotensin System Studies
- Receptor Mechanisms and Signaling
- Mercury impact and mitigation studies
- Heavy Metal Exposure and Toxicity
- Hormonal Regulation and Hypertension
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Traumatic Brain Injury and Neurovascular Disturbances
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neuroscience of respiration and sleep
- Neuropeptides and Animal Physiology
- Atherosclerosis and Cardiovascular Diseases
- Biochemical effects in animals
- Cardiac electrophysiology and arrhythmias
- Intracranial Aneurysms: Treatment and Complications
- Cardiovascular Health and Disease Prevention
- Heart Rate Variability and Autonomic Control
- Protein Hydrolysis and Bioactive Peptides
- Sodium Intake and Health
- Peroxisome Proliferator-Activated Receptors
- Fatty Acid Research and Health
Universidad Autónoma de Madrid
2015-2025
Hospital Universitario La Paz
2015-2025
Centro de Investigación en Red en Enfermedades Cardiovasculares
2016-2025
Hospital La Paz Institute for Health Research
2012-2025
Centro de Investigación Biomédica en Red
2017-2025
Instituto de Salud Carlos III
2017-2021
Instituto de Investigación en Ciencias de la Alimentación
2016-2018
Hospital Universitario de Torrejón
2016-2018
Universidade Federal do Espírito Santo
2011-2016
Universidad Rey Juan Carlos
2007-2016
Aims: This study evaluates a possible relationship between reactive oxygen species (ROS) and cyclooxygenase (COX)-2-derived products in conductance resistance arteries from hypertensive animals. Angiotensin II (Ang II)-infused mice or spontaneously rats treated with the NAD(P)H Oxidase inhibitor apocynin, mitochondrion-targeted SOD2 mimetic Mito-TEMPO, superoxide dismutase analog tempol, COX-2 Celecoxib were used. Results: Apocynin, treatments prevented Ang II-induced hypertension, increased...
Increased cardiovascular risk after mercury exposure has been described, but the underlying mechanisms are not well explored. We analyzed effects of chronic to low concentrations on endothelium-dependent responses in aorta and mesenteric resistance arteries (MRA). Wistar rats were treated with chloride (1st dose 4.6 microg/kg, subsequent 0.07 microg.kg(-1).day(-1) im, 30 days) or vehicle. Blood levels at end treatment 7.97 +/- 0.59 ng/ml. Mercury treatment: 1) did affect systolic blood...
Regular physical activity is an effective non-pharmacological therapy for prevention and control of hypertension. We investigated the effects aerobic exercise training in vascular remodelling mechanical functional alterations coronary small mesenteric arteries from spontaneously hypertensive rats (SHR).Normotensive Wistar Kyoto (WKY), SHR trained on a treadmill 12 weeks were used to evaluate structural, properties.Exercise did not affect lumen diameter, wall thickness wall/lumen ratio but...
Background and Purpose Toll‐like receptor 4 ( TLR 4) signalling contributes to inflammatory cardiovascular diseases, but its role in hypertension the associated vascular damage is not known. We investigated whether activation contributed angiotensin II AngII )‐induced structural, mechanical functional alterations. Experimental Approach was infused (1.44 mg·kg −1 ·day , s.c.) for 2 weeks C57BL6 mice, treated with a neutralizing anti‐ antibody or IgG (1 μg·day ); systolic BP (SBP) aortic...
Branched-chain amino acids (BCAA: leucine, isoleucine and valine) are essential implicated in glucose metabolism maintenance of correct brain function. Elevated BCAA levels can promote an inflammatory response peripheral blood mononuclear cells. However, there no studies analysing the direct effects on endothelial cells (ECs) its possible modulation vascular In vitro ex vivo were performed human ECs aorta from male C57BL/6J mice, respectively. ECs, (6 mmol/L) increased eNOS expression,...
Hypertension is considered as a low-grade inflammatory disease, with adaptive immunity being an important mediator of this pathology. TLR4 may have role in the development several cardiovascular diseases; however, little known about its participation hypertension. We aimed to investigate whether activation due increased activity renin-angiotensin system (RAS) contributes hypertension and associated endothelial dysfunction. For this, we used aortic segments from Wistar rats treated...
Aims: Vascular stiffness, structural elastin abnormalities, and increased oxidative stress are hallmarks of hypertension. Lysyl oxidase (LOX) is an crosslinking enzyme that produces H2O2 as a by-product. We addressed the interplay between LOX, stress, vessel elastin. Results: Angiotensin II (Ang II)-infused hypertensive mice spontaneously rats (SHR) showed vascular LOX expression stiffness abnormal structure. Mice over-expressing in smooth muscle cells (TgLOX) exhibited similar mechanical...
microRNAs are master regulators of gene expression with essential roles in virtually all biological processes. miR-217 has been associated aging and cellular senescence, but its role vascular disease is not understood. Approach Results: We have used an inducible endothelium-specific knock-in mouse model to address the function atherosclerosis. reduced NO production promoted endothelial dysfunction, increased blood pressure, exacerbated atherosclerosis proatherogenic apoE-/- mice. Moreover,...
The present study analyses the influence of hypertension and endothelium on effect induced by hydrogen peroxide (H 2 O ) basal tone in aortic segments from normotensive Wistar‐Kyoto (WKY) spontaneously hypertensive rats (SHR) 6‐month‐old, as well possible mechanisms involved. Single (1 m or cumulative (100 n –10 concentrations H produced a transient contraction concentration‐dependent increase tone, respectively, WKY SHR. In both cases, contractions were higher intact than rats, increased...
This study analyzes the time course of changes induced by subarachnoid hemorrhage (SAH) in sensitivity cat cerebral arteries to noradrenaline and serotonin. Cerebral displayed a supersensitivity these amines, which was most marked 3 days after experiment then gradually disappeared. The serotonin greater longer than response noradrenaline. increase vascular contractile SAH similar that seen superior cervical ganglionectomy or intracisternal injections 6-hydroxydopamine. It is suggested may be...
The aim of the present study was to analyze possible involvement vasoconstrictors prostanoids on reduced endothelium-dependent relaxations produced by chronic administration aldosterone in Wistar Kyoto rats (WKY) and spontaneously hypertensive (SHR). For this purpose, acetylcholine (ACh) aortic segments from both strains were analyzed absence presence cyclooxygenase-1 (COX-1) COX-2 inhibitor indomethacin, specific NS-398, TP receptor antagonist (SQ 29 548), thromboxane A 2 (TXA ) synthase...
Angiotensin II (Ang II) modulates vasomotor tone, cell growth, and extracellular matrix deposition. This study analyzed the effect of atorvastatin in possible alterations induced by Ang on structure mechanics mesenteric resistance arteries signaling mechanisms involved. Wistar rats were infused with (100 ng/kg per day, SC minipumps, 2 weeks) or without (5 mg/kg day). increased blood pressure plasmatic malondialdehyde levels. Compared controls, from II-treated showed following: (1) decreased...
Organ regeneration is preceded by the recruitment of innate immune cells, which play an active role during repair and regrowth. Here, we studied macrophage subtypes organ in zebrafish, animal model with a high regenerative capacity. We identified subpopulation expressing Wilms tumor 1b (wt1b), accumulates within regenerating tissues. This wt1b+ population exhibited overall pro-regenerative gene expression profile different migratory behavior compared to remainder macrophages. Functional...
Abstract Vascular stiffness is a major cause of cardiovascular disease during normal aging and in Hutchinson–Gilford progeria syndrome (HGPS), rare genetic disorder caused by ubiquitous progerin expression. This mutant form lamin A causes premature associated with alterations that lead to death at an average age 14.6 years. We investigated the mechanisms underlying vessel Lmna G609G/G609G mice expression, tested effect treatment nitrites. also bred LCS/LCS Tie2Cre +/tg SM22αCre mice, which...
The present study was designed to analyse whether hypertension alters the involvement of cyclooxygenase-2-derived mediators in phenylephrine-induced vasoconstrictor responses.Vascular reactivity experiments were performed aortic segments from normotensive, Wistar-Kyoto, and spontaneously hypertensive rats (SHR); protein expression measured by western blot and/or immunohistochemistry, prostaglandin F2alpha (PGF2alpha), 8-isoprostane prostacyclin release determined enzyme immunoassay...