A5101500523- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Biochemical Analysis and Sensing Techniques
- Pancreatic function and diabetes
- Protein Tyrosine Phosphatases
- Adipokines, Inflammation, and Metabolic Diseases
- Sleep and Wakefulness Research
- Diet and metabolism studies
- Metabolism, Diabetes, and Cancer
- Receptor Mechanisms and Signaling
- Pharmacological Effects of Natural Compounds
- Antibiotic Resistance in Bacteria
- Retinal Diseases and Treatments
- Peroxisome Proliferator-Activated Receptors
- Cancer, Hypoxia, and Metabolism
- Diabetes Treatment and Management
- Parkinson's Disease Mechanisms and Treatments
- Pain Mechanisms and Treatments
- Cholesterol and Lipid Metabolism
- Advanced Glycation End Products research
- Fatty Acid Research and Health
- Antibiotics Pharmacokinetics and Efficacy
- Galectins and Cancer Biology
- Retinal Development and Disorders
- Biochemical effects in animals
Japan Tobacco (Japan)
2005-2021
National Institute for Physiological Sciences
2012
Kariya Toyota General Hospital
2012
Food Research Institute
2003-2009
Chubu University
2007
Japan Tobacco (United States)
2004
United States Military Academy
2001
Melanin-concentrating hormone (MCH) is a 19-aa cyclic neuropeptide originally isolated from chum salmon pituitaries. Besides its effects on the aggregation of melanophores in fish several lines evidence suggest that mammals MCH functions as regulator energy homeostasis. Recently, groups reported identification an orphan G protein-coupled receptor for (MCH-1R). We hereby report second human termed MCH-2R, which shares about 38% amino acid identity with MCH-1R. MCH-2R displayed high-affinity...
Melanin-concentrating hormone (MCH) is a cyclic amino acid neuropeptide localized in the lateral hypothalamus. Although MCH thought to be an important regulator of feeding behavior, involvement this peptide body weight control has been unclear. To examine role development obesity, we assessed effect chronic intracerebroventricular infusion C57BL/6J mice that were fed with regular or moderately high-fat (MHF) diets. Intracerebroventricular (10 microg/day for 14 days) caused slight but...
Melanin-concentrating hormone (MCH) is a cyclic orexigenic peptide expressed in the lateral hypothalamus. Recently, we demonstrated that chronic intracerebroventricular infusion of MCH induced obesity accompanied by sustained hyperphagia mice. Here, analyzed mechanism MCH-induced comparing animals fed ad libitum with pair-fed and control animals. Chronic significantly increased food intake, body weight, white adipose tissue (WAT) mass, liver mass libitum-fed mice on moderately high-fat diet....
Melanin-concentrating hormone (MCH) is a cyclic orexigenic peptide expressed in the lateral hypothalamus, which plays an important role regulating energy balance. To elucidate physiological of MCH obesity development, present study examined effect selective MCH1 receptor (MCH1R) antagonist diet-induced mouse model. The MCH1R has high affinity and selectivity for MCH-1R potently inhibits intracerebroventricularly injected MCH-induced food intake Sprague Dawley rats. Chronic...
Aim: Protein tyrosine phosphatase 1B (PTP1B), a negative regulator of insulin signalling, is novel therapeutic target for type 2 diabetes mellitus. We evaluated in vitro and vivo the pharmacological profiles new PTP1B inhibitor, JTT‐551: monosodium ({[5‐(1,1‐dimethylethyl)thiazol‐2‐yl]methyl} {[(4‐{4‐[4‐(1‐propylbutyl)phenoxy]methyl}phenyl)thiazol‐2‐yl]methyl}amino)acetate. Methods: inhibitory activity inhibition mode were assayed with p ‐nitrophenyl phosphate as substrate, selectivity...
Aim: JTP‐426467 was identified as a result of screening in search selective antagonist for peroxisome proliferator‐activated receptor γ (PPARγ). We examined whether functioned PPARγ vitro and vivo investigated physiological effects JTP‐426467. Methods: The effect studied cell‐based reporter assay an adipocyte differentiation assay. Target mRNA expression levels were determined by branched DNA (bDNA) To examine the , competitive study between BRL49653 (rosiglitazone), agonist, performed using...
Protein tyrosine phosphatase 1B (PTP1B) is a negative regulator of leptin signaling as well insulin signaling. JTT-551 new PTP1B inhibitor, which reported to improve glucose metabolism by enhancement We have evaluated an antiobesity effect using diet-induced obesity (DIO) mice. A single administration was provided DIO mice with or without leptin, and were given food containing for six weeks. treatment enhanced the inhibition signal transducer activator transcription 3 (STAT3) phosphorylation...
Spontaneously Diabetic Torii (SDT) rat is a new model of non-obese type 2 diabetes. SDT rats show severe ocular complications such as cataracts, tractional retinal detachment with fibrous proliferation and massive haemorrhaging in the anterior chamber. In present study, blood glucose levels were controlled order to examine whether these are caused by hyperglycaemia.SDT treated an insulin implant control glucose. To evaluate function, we used electroretinograms (ERG) measured vascular...
Melanin-concentrating hormone (MCH) is an orexigenic neuropeptide expressed in the lateral hypothalamus that involved feeding and body weight regulation. Intracerebroventricular infusion of a peptidic MCH1 receptor antagonist ameliorated obesity murine models. Recently, small molecule antagonists have been developed characterized for treatment obesity. However, little known mechanism anti-obesity effects antagonists.To examine mechanisms action effect more precisely, we conducted...
Activation of protein kinase C (PKC) is thought to play an important role in the pathogenesis diabetic microvascular complications. PKC-beta elevated hyperglycaemic conditions, both vivo and vitro. In this study, pharmacological effects a novel isoform selective inhibitor, JTT-010 ((2R)-3-(2-aminomethyl-2,3-dihydro-1H-3a-azacyclopenta(a)inden-8-yl)-4-phenylaminopyrrole-2,5-dione monomethanesulphonate), on neuropathy were examined.PKC inhibitory activity was evaluated with enzyme assay. For...
Melanin-concentrating hormone (MCH) is a cyclic orexigenic neuropeptide predominantly expressed in the lateral hypothalamus. We investigated roles of MCH1 receptor signalling ovariectomy (OVX)-induced obesity female C57BL/6J mice, an animal model postmenopausal obesity.The effects blocking via on OVX-induced was by using Mch1r deficient (KO) mice and chronic treatment with selective antagonist.OVX induced body weight gain increases visceral fat liver; these were attenuated following OVX KO...
Spontaneously Diabetic Torii-fa/fa (SDT fatty) rat is a new model of obese type 2 diabetes. SDT fatty exhibits obesity associated with hyperphagia. In this study, rats were subjected to pair-feeding SDT-+/+ (SDT) from 6 22 weeks age. The ratio visceral fat weight subcutaneous (V/S) decreased at 12 age in the rats. intraperitoneal such as epididymal and retroperitoneal decreased, whereas mesenteric had no change. Cell size tended decrease. Glucose oxidation level was recovered similar that...
The Spontaneously Diabetic Torii (SDT) rat has recently been established as a new model of non-obese type 2 diabetes. In this study, we examined changes in hepatic glucose metabolism prediabetic and diabetic SDT rats compared with age-matched control rats. state was confirmed at 16 weeks age, the 24 32 age. Decreases glucokinase mRNA levels activity were observed state. state, glycogen synthase content also decreased rat. addition to above changes, phosphorylase gluconeogenetic enzyme...
ABSTRACT Spontaneously Diabetic Torii (SDT) fatty rats, made by introducing the fa allele of Zucker rat into SDT genome, represent a new model obese type 2 diabetes. fa/fa (SDT fatty) rats exhibit overt obesity, hyperglycemia, and hyperlipidemia from about six weeks age, this is associated with hyperphagia an induced disorder leptin action. The present study was conducted to elucidate whether suppression can improve reduce abnormalities in rats. were subjected pair‐feeding +/ + 6 26 effects...
Some nutrients, such as carbohydrate, fat and protein, are known to stimulate satiety. However, the effect of sn-2-monoacylglycerol (2-MG), one digestive products triglycerides, on food intake is still unclear. In present study, effects 2-MG diarrhea were evaluated compared with long-chain fatty acid (LCFA) in rats by intrajejunal infusion. Intrajejunal infusion reduced intake. addition, did not induce at condition that it comparably LCFA. These results suggest stimulates satiety without...
We investigated the effects of JTT-130 on glucose and lipid metabolism independent suppression feeding by comparing with pair-fed animals. Male Zucker diabetic fatty (ZDF) rats were divided into control, treatment, groups. The fed a regular powdered diet or without as food admixture for 6 weeks. compared in treatment group those group. Results . Hyperglycemia ZDF was prevented both groups, but prevention became poor time. Moreover, reduction plasma cholesterol levels observed only showed...
愛知県西三河地区の市中病院で2008年から2010年までの3年間に分離同定され，抗菌薬感受性検査を実施した緑膿菌1343株を対象に，抗菌薬感受性結果の推移を分析した．その結果，カルバペネム系抗菌薬に対する耐性率はIPM/CSで6.4% vs 3.3% (2008年対2010年のデータを対比，以下同様)，MEPMで2.7% 1.4%と低下傾向を示した．同時にCPFXの耐性率も13.4% 5.5%と低下した．アミノグリコシド系抗菌薬であるAMKの耐性率は0.2% 0.6%と低率を維持した．対照的にβ–ラクタム系抗菌薬に対する耐性率は上昇傾向を示した．この推移は同期間におけるカルバペネム系抗菌薬の投与量の減少や相対的な第3，第4世代のセフェム系抗菌薬の使用比の増加と連動していた．緑膿菌の耐性率の改善の背景には，カルバペネム系抗菌薬の適正使用に向けた取り組みのみならず，感染制御活動の成果やDPC導入に伴う入院期間の短縮など複合的な要因が挙げられる．多剤耐性緑膿菌の検出は他院からの持ち込みの1例のみであった．今後も抗菌薬感受性サーベイランスに基づく抗菌薬の適正使用に向けた質の高い活動の継続が必要である．
Spontaneously Diabetic Torii (SDT) rat is a model of severe type 2 diabetes and its complications. These characteristics SDT are very useful to research diseases; however, the slow onset may limit usefulness this animal model. To solve problem, we performed bilateral midbrain transection on rats evaluated whether hyperphagia accelerates diabetes. By severing ascending fibers from nucleus tractus solitarius limbic regions through ventral dorsal tegmental regions, food consumption was...