A5018053115- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- Nanoparticle-Based Drug Delivery
- Plasmonic and Surface Plasmon Research
- Graphene and Nanomaterials Applications
- Nanoparticles: synthesis and applications
- Quantum-Dot Cellular Automata
- Cell Adhesion Molecules Research
- Anaerobic Digestion and Biogas Production
- Acne and Rosacea Treatments and Effects
- Microbial Fuel Cells and Bioremediation
- Urticaria and Related Conditions
- Cellular Mechanics and Interactions
- Dermatology and Skin Diseases
- Protein Structure and Dynamics
- Nanofabrication and Lithography Techniques
- Nanopore and Nanochannel Transport Studies
- Wastewater Treatment and Nitrogen Removal
- RNA and protein synthesis mechanisms
- Force Microscopy Techniques and Applications
Kent State University
2020-2025
Asian Institute of Technology
2018
Kathmandu University
2016-2017
Novel magnetic composite nanoparticles (MCPs) were successfully synthesized by ex situ conjugation of ZnO (ZnO NPs) and Fe3O4 NPs using trisodium citrate as linker with an aim to retain key properties both viz. inherent selectivity towards cancerous cell superparamagnetic nature, respectively, on a single system. Successful characterization was done XRD, TEM, FTIR, VSM analyses. analysis showed similar profile thus obtained MCPs that naked reduction in saturation magnetization 16.63 emu/g....
Nanoparticles (NPs) are receiving increasing interest in biomedical research owing to their comparable size with biomolecules, novel properties and easy surface engineering for targeted therapy, drug delivery selective treatment making them a better substituent against traditional therapeutic agents. ZnO NPs, despite other applications, also show anticancer property which makes it good option over metal oxide NPs. NPs were synthesized by chemical precipitation technique, then modified using...
For proteins and DNA secondary structures such as G-quadruplexes i-motifs, nanoconfinement can facilitate their folding increase structural stabilities. However, the properties of physiologically prevalent B-DNA duplex have not been elucidated inside nanocavity. Using a 17-bp in form hairpin stem, here, we probed unfolding transitions origami Compared to free solution, nanocage with 15 × nm cross section showed drastic decrease mechanical (20 → 9 pN) thermodynamic (25 6 kcal/mol) Free energy...
Cell motions such as migration and change in cellular morphology are essential activities for multicellular organism response to environmental stimuli. These a result of coordinated clustering/declustering integrin molecules at the cell membrane. Here, we prepared DNA origami nanosprings modulate by targeting clustering molecules. Each nanospring was modified with arginyl-glycyl-aspartic acid (RGD) domains spacing that when is coiled, RGD ligands trigger molecules, which changes motions. The...
Mesoscopic objects ranging from molecular machinery to cells are prevalent in nature. Unlike atomic and nanoscopic that do not have pronounced mechanical properties due their small sizes, mesoscale substances demonstrate unique features can interfere with cell functions, particularly those a nature such as migrations. Here, we caging/uncaging effects DNA origami nanospring system precisely controls cancer By leveraging programming language, our work demonstrates the creation of logic gates...
Abstract The intrinsic complexity of many mesoscale (10–100 nm) cellular machineries makes it challenging to elucidate their topological arrangement and transition dynamics. Here, we exploit DNA origami nanospring as a model system demonstrate that tens piconewton linear force can modulate higher-order conformation dynamics molecular assemblies. By switching between two chemical structures (i.e., duplex tetraplex DNA) in the junctions adjacent modules, corresponding stretching or compressing...
While single-molecule sensing has offered ultimate mass sensitivity at the precision of individual molecules, it requires a longer time to detect analytes lower concentrations when analyte binding probes becomes diffusion-limited. Here, we solved this accuracy problem in concentration determination by using DNA homopolymers, which up 473 identical elements (DNA hairpins) were introduced rolling circle amplification. Surprisingly, homopolymers containing as few 10 tandem hairpins displayed...
Using a force-jump approach in optical tweezers, the spring constants and dynamic recoiling responses of DNA nanosprings can be recorded.
Shear force in a homogenizer mechanically unfolds an ensemble set of biomolecular structures.
Background Corticosteroids are highly effective drugs with anti-inflammatory and immunosuppressive properties. Due to this, they have become a mainstay of pharmacotherapy in dermatology. However, improper long term uses associated number serious adverse effects. Objective To investigate the corticosteroids use pattern, effects various factors dermatological practice. Method A cross-sectional study was conducted dermatology department tertiary care hospital. All patients using at least one...
Common in biomacromolecules, kinetically trapped misfolded intermediates are often detrimental to the structures, properties, or functions of proteins nucleic acids. Nature employs chaperone but not acids escort correct conformations. Herein, we constructed a Jablonski-like diagram mechanochemical cycle which individual DNA hairpins were mechanically unfolded high-energy states, into and catalytically relaxed back ground-state by chaperone. The capacity catalytic relaxation was demonstrated...
Abstract Small molecules can inhibit cellular processes such as replication and transcription by binding to the promoter regions that are prone form G-quadruplexes. However, since G-quadruplexes exist throughout human genome, G-quadruplex binders suffer from specificity issues. To tackle this problem, a binder (Pyridostatin, or PDS) is conjugated with ligand (Polyamide, PA) specifically recognize DNA sequences flanking forming region. The mechanism of hybrid hTERT region (hTERT 5–12) then...
Abstract 8‐oxoguanines (8‐oxoG) in cells form compromised G‐quadruplexes (GQs), which may vary GQ mediated gene regulations. By mimicking molecularly crowded cellular environment using 40% DMSO or sucrose, here it is found that oxidized human telomeric GQs have stabilities close to the wild‐type (WT) GQs. Surprisingly, while WT show negative formation cooperativity between a Pt(II) binder and environment, positive observed for formation. Single‐molecule mechanical unfolding reveals 8‐oxoG...