A5074433217- Natural Antidiabetic Agents Studies
- Bioactive natural compounds
- Biofuel production and bioconversion
- Ethnobotanical and Medicinal Plants Studies
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Phytochemical Studies and Bioactivities
- Metabolism, Diabetes, and Cancer
- Chemotherapy-induced cardiotoxicity and mitigation
- Computational Drug Discovery Methods
- Cell death mechanisms and regulation
- Plasma Applications and Diagnostics
- Phytochemicals and Medicinal Plants
- PARP inhibition in cancer therapy
- Enzyme Production and Characterization
- Diagnosis and treatment of tuberculosis
- Graphene and Nanomaterials Applications
- Nanoparticle-Based Drug Delivery
- Heat shock proteins research
- Nanoparticles: synthesis and applications
- Medicinal Plants and Neuroprotection
- Biological and pharmacological studies of plants
- Anesthesia and Pain Management
- Botanical Research and Applications
- Cancer Risks and Factors
Kathmandu University
2015-2025
Dhulikhel Hospital
2021
Tokyo University of Agriculture
2009
National Institute of Advanced Industrial Science and Technology
2006-2008
Institute of Pathology Celle
2007
Abstract Purpose: Ashwagandha is regarded as a wonder shrub of India and commonly used in Ayurvedic medicine health tonics that claim its variety health-promoting effects. Surprisingly, these claims are not well supported by adequate studies, the molecular mechanisms action remain largely unexplored to date. We undertook study identify characterize antitumor activity leaf extract ashwagandha. Experimental Design: Selective tumor-inhibitory (i-Extract) was identified vivo tumor formation...
The development of digital technologies and the evolution open innovation approaches have enabled creation diverse virtual organizations enterprises coordinating their activities primarily online. platform titled "International Natural Product Sciences Taskforce" (INPST) was established in 2018, to bring together collaborative environment individuals interested natural product scientific research, empower interactions by using communication tools.In this work, we present a general overview...
There is growing interest in the use of plants for treatment and prevention cancer. Medicinal are currently being evaluated as source promising anticancer agents. In this paper, we have investigated potential plant Allium wallichii, a native to Nepal at elevations 2300–4800 m. This first study its kind mentioned. The dried was extracted aqueous ethanol. Phytochemical screening, anti-microbial assay, anti-oxidant cytotoxicity assay flow-cytometric analysis were done analyzing different...
Novel magnetic composite nanoparticles (MCPs) were successfully synthesized by ex situ conjugation of ZnO (ZnO NPs) and Fe3O4 NPs using trisodium citrate as linker with an aim to retain key properties both viz. inherent selectivity towards cancerous cell superparamagnetic nature, respectively, on a single system. Successful characterization was done XRD, TEM, FTIR, VSM analyses. analysis showed similar profile thus obtained MCPs that naked reduction in saturation magnetization 16.63 emu/g....
Nanoparticles (NPs) are receiving increasing interest in biomedical research owing to their comparable size with biomolecules, novel properties and easy surface engineering for targeted therapy, drug delivery selective treatment making them a better substituent against traditional therapeutic agents. ZnO NPs, despite other applications, also show anticancer property which makes it good option over metal oxide NPs. NPs were synthesized by chemical precipitation technique, then modified using...
The present study was undertaken to find the phytochemical presence, antioxidant potential, antimicrobial activity, and anticancer activity of methanol extract Berberis Aristata. Plant showed presence antioxidants like alkaloid, saponin, terpenoids, coumarin, flavonoids, tannin, glycoside steroid. Further, assay DPPH (Diphenyl-2-picrylhydrazyl) assay, hydrogen peroxide reducing power done. free radical Scavenging Activity expressed in % inhibition with L Ascorbic acid as standard showing...
Cancer is one of the major health problems worldwide and medicinal plants constitute a common alternative for cancer treatment having no or fewer side effects. This study aimed to assess total phenolic (TPC) flavonoid (TFC) contents, antioxidant, biological activities (especially antibacterial, antifungal, anticancer), chemical composition methanol extract M. napaulensis DC. bark (MNBM). first evaluating its anticancer activity by LC–MS/MS analysis. TPC, TFC, antimicrobial, were determined...
In today’s world, search for natural medicines is increasing as a result of drug resistance pathogens and also due to negative consequences antibiotic. Presence phytochemicals, antioxidant potential antimicrobial activity Artocarpus heterophyllus was carried out in this study. Leaf plant subjected warm extraction with three different solvents namely methanol, aqueous methanol ethyl acetate. extract showed the presence coumarin, alkaloid, terpenoid solvent; tannin, saponin terpenoids acetate...
Breast cancer is the most common among women around world. Human Epidermal growth factor Receptor-2 (HER2) a membrane tyrosine kinase overexpressed in 30% of human breast cancers; thus, it serves as an important drug target. Currently available HER2 inhibitor lapatinib targets ATP binding site cytoplasmic domain, blocking autophosphorylation and activation HER-2. However, causes side effects like diarrhea, nausea, rash possible liver toxicity. As phytochemicals have fewer are relatively...
Ethnomedicinal plants are being used as a source of medicine from ancient time but they lack the proof modern scientific evidence for their effectiveness. This study focuses on evaluation phytochemical, antimicrobial, antioxidant properties one ethnomedicinal plant Agave americana Dhulikhel region Nepal. The extract was prepared using solvent-based warm soxhlet extraction leaves and antimicrobial activity against six different non-resistant clinical isolates bacteria (Staphylococcus aureus,...
Cellulolytic/Xylanolytic microorganisms such as bacteria and fungi are accountable for conversion of lignocellulosic biomass in soil. Despite this vast number cellulose/xylanase producers, there is a deficiency that can produce significant amount cellulase/xylanase enzyme to proficiently degrade cellulose/xylan fermentable sugars. Although have extremely high natural diversity, which bestowsthem with the aptitude stable enzymes, little emphasis has been given production from bacteria. Seven...
Research on medicinal plants are important to Nepal because most of its rural population relies it as mode medicine. Medicinal namely Swertia chirayita and Dendrobium amoenum were collected from mid hills Nepal. The present study was undertaken find the antimicrobial activity, phytochemical presence their cytotoxicity in different extraction medium. percentage yield highest warm methanol with 12.6%, followed by ethyl acetate lowest for cold methanol. Plant extract showed antioxidants like...
There is growing interest in the use plants for treatment and prevention of cancer are currently being evaluated as promising anticancer agents. In this paper, we investigated anti-microbial, anti-oxidant, phytotoxic immunomodulatory plant Bauhinia variegata also established presence important phytoconstituents which might signify its property. Phytochemical screening, anti-microbial assay, phytotoxicity anti-oxidant assay analysis was done according to protocol at Department Biotechnology,...
Breast cancer (BC) is the most common type of worldwide, being a major cause morbidity in female. In Nepal it second among females perimenopausal age group. More than one quarter BC diagnosed young Nepalese female, with familial history breast cancer, early pregnancy, longer lactation and estrogen exposure often tumors showing aggressive biological behaviors. Anthracyclines (Doxorubicin) based treatment regime were reported to cardiotoxicity by increasing intramyocardial free radical...
<title>Abstract</title> Background Cancer is one of the major health problems worldwide and medicinal plants constitute a common alternative for cancer treatment having no or less side effects. This study was aimed to assess total phenolic (TPC) flavonoid (TFC) contents, antioxidant, biological activities (especially antibacterial, antifungal anticancer) chemical composition methanol extract <italic>M. napaulensis</italic> DC. bark (MNBM). first evaluating its anticancer activity by LC-MS/MS...
Ser315Thr mutations in genes encoding the mycobacteria catalase-peroxidase (KatG) has been associated with major resistance to isoniazid (INH) Mycobacterium tuberculosis (MTB). Also G/C polymorphisms INT4 region of solute carrier family 11 member 1 gene (SLC11A1) and susceptibility towards (TB) demonstrated worldwide. 24 drug resistant MTB culture positive samples whole?blood were collected from different TB patients Central Development Region Nepal 2009. A Polymerase Chain Reaction (PCR) -...
The anticancer properties of plasma were studied by treating Dulbecco's modified Eagle's medium (DMEM) with cold atmospheric pressure (CAPP). CAPP was generated using high voltage power supply (11.75 kV) at an operating frequency 50 Hz. DMEM treated argon as the process gas for different exposure time ranging from 0.5 to 3 minutes. media transferred Henrietta Lacks (HeLa) and Human Embryonic Kidneys 293 (HEK 293) cells. viability cancer cells observed 3-(4, 5-dimethylthiazol-2-yl)-2,...
Background: Chemotherapy agents alter serum lipids and C-peptide in cancer patients increased incidence of cardiovascular disorders diabetes, although mechanisms for these are not known. Diabetes riskis the highly concerned chemotherapy induced complications among patients. This study is aimed to evaluate breast receiving chemotherapy.Methods: cross-sectional includes160 individuals comprising 80 controls with age ranges from 24 76 years. Patients received at least 3 cycles chemotherapy....
In this paper, we demonstrated the application of atmospheric pressure argon plasma jet (APAPJ) in biomedical science such as cancer therapy and engineering surface modification polystyrene Petri dishes for animal cell culture. We observed that APAPJ, when exposed to breast line (MDA-MB-231) 60 seconds at a distance ~ 2.5 cm, reduced cells by 57%. This reduction concentration cancer-killing property APAPJ. Further optimization APAPJ's various operational parameters in-depth biochemical study...