A5075922510- Vibrio bacteria research studies
- Yersinia bacterium, plague, ectoparasites research
- Plant Pathogens and Fungal Diseases
- Bacillus and Francisella bacterial research
- Entomopathogenic Microorganisms in Pest Control
- Vector-borne infectious diseases
- Bacteriophages and microbial interactions
- Leptospirosis research and findings
- Bacterial biofilms and quorum sensing
- Viral Infections and Outbreaks Research
- Gut microbiota and health
- Insect symbiosis and bacterial influences
- Plant pathogens and resistance mechanisms
- Viral Infections and Vectors
- Research on Leishmaniasis Studies
- Plant and animal studies
- Zoonotic diseases and public health
- Insect Pest Control Strategies
- Primate Behavior and Ecology
- Polyamine Metabolism and Applications
- Toxoplasma gondii Research Studies
- Insects and Parasite Interactions
- Mycotoxins in Agriculture and Food
- Research on scale insects
- Inhalation and Respiratory Drug Delivery
National Institute of Allergy and Infectious Diseases
2015-2024
National Institutes of Health
2015-2024
University of Montana
2018-2023
Genesis Laboratories
2016
Stony Brook University
2014
Centre National de la Recherche Appliquée au Developpement Rural
2014
National Center for Infectious Diseases
2009-2010
Laboratory of Molecular Genetics
2010
Albany Medical Center Hospital
2010
Bacteria in natural environments and infections are often found cell aggregates suspended polymer-rich solutions, aggregation can promote bacterial survival stress resistance. One mechanism, called depletion aggregation, is driven by physical forces between bacteria high concentrations of polymers the environment rather than activity per se. As such, aggregated mechanism will disperse when polymer fall unless other adhesion mechanisms supervene. Here we investigated whether actuate...
Rickettsia rickettsii is an obligate intracellular pathogen that the causative agent of Rocky Mountain spotted fever. Strains R. differ dramatically in virulence. In a guinea pig model infection, severity disease as assessed by fever response varies from most virulent, Sheila Smith, to Iowa, which causes no To identify potential determinants virulence rickettsii, genomes two additional strains were sequenced for comparison known sequences (comparative genome sequencing [CGS]). Morgan and R...
As human population density continues to increase exponentially, speeding the reduction and fragmentation of primate habitat, greater human‐primate contact is inevitable, making higher rates pathogen transmission likely. Anthropogenic effects are particularly evident in Madagascar, where a diversity endemic lemur species threatened by rapid habitat loss. Despite these risks, knowledge how anthropogenic activities affect exposure pathogens limited. To improve our understanding this interplay,...
Monitoring the extracellular environment for danger signals is a critical aspect of cellular survival. However, released by dying bacteria and mechanisms use threat assessment remain largely unexplored. Here, we show that lysis Pseudomonas aeruginosa cells releases polyamines are subsequently taken up surviving via mechanism relies on Gac/Rsm signaling. While intracellular spike in cells, duration this varies according to infection status cell. In bacteriophage-infected maintained at high...
Background Among the families of enteric bacteria are globally important diarrheal agents. Despite their potential for zoonotic and environmental transmission, few studies have examined epidemiology these pathogens in rural systems characterized by extensive overlap among humans, domesticated peridomestic animals. We investigated patterns infection with Enterotoxigenic Escherichia coli, Shigella spp., Salmonella enterica, Vibrio cholerae, Yersinia spp. (enterocolitica, pseudotuberculosis)...
Abstract Mitochondrial genomes can provide valuable information on the biology and evolutionary histories of their host organisms. Here, we present characterize complete coding regions 107 mitochondrial (mitogenomes) cicadas (Insecta: Hemiptera: Auchenorrhyncha: Cicadoidea), representing 31 genera, 61 species, 83 populations. We show that all cicada mitogenomes retain organization gene contents thought to be ancestral in insects, with some variability among clades length a region between...
The highly infectious bacterium Francisella tularensis is a facultative intracellular pathogen and the causative agent of tularemia. TolC, which an outer membrane protein involved in drug efflux type I secretion, required for virulence F. live vaccine strain (LVS) mice. Here, we show that LVS DeltatolC mutant colonizes livers, spleens, lungs mice infected intradermally or intranasally, but it present at lower numbers these organs than those with parental LVS. For both routes infection,...
The fungal genus Massospora (Zoopagomycota: Entomophthorales) includes more than a dozen obligate, sexually transmissible pathogenic species that infect cicadas (Hemiptera) worldwide. At least two are known to produce psychoactive compounds during infection, which has garnered considerable interest for this enigmatic genus. As with many Entomophthorales, the evolutionary relationships and host associations of spp. not well understood. acquisition M. diceroproctae from Arizona, tettigatis...
ABSTRACT We compared the growth characteristics of a virulent Rickettsia rickettsii strain (Sheila Smith) to an attenuated R. stain (Iowa) and non-pathogenic species ( montanensis ) in primary human dermal microvascular endothelial cells (HDMEC). All replicated Vero cells, however, only Sheila Smith productively HDMECs. The Iowa showed minimal replication over 24-h period, while lost viability induced lysis HDMECs via rapid programmed cell death response. Both strains, but not , interferon-1...
Francisella tularensis, the causative agent of tularemia, interacts with host cells innate immunity in an atypical manner. For most Gram-negative bacteria, release lipopolysaccharide (LPS) from their outer membranes stimulates inflammatory response. When LPS attenuated live vaccine strain (LVS) or highly virulent Schu S4 F. tularensis was incubated human umbilical vein endothelial cells, neither species induced expression adhesion molecule E-selectin secretion chemokine CCL2. Moreover, a...
Various bacterial pathogens activate the endothelium to secrete proinflammatory cytokines and recruit circulating leukocytes. In contrast, there is a distinct lack of activation these cells by Francisella tularensis, causative agent tularemia. Given importance endothelial in facilitating innate immunity, we investigated ability attenuated live vaccine strain virulent Schu S4 F. tularensis inhibit response HUVECs. Living did not stimulate secretion chemokine CCL2 HUVECs, whereas material...
Abstract When eukaryotic cells are killed by pathogenic microorganisms, damage-associated and pathogen-associated signals generated that alert other of nearby danger. Bacteria can detect the death their kin; however, how bacteria make threat assessments cellular injury is largely unexplored. Here we show polyamines released lysed serve as molecules in Pseudomonas aeruginosa . In response to exogenous polyamines, Gac/Rsm cyclic-di-GMP signaling activated intracellular polyamine levels...
Abstract Bacteria causing chronic infections are often found in cell aggregates suspended polymer secretions, and aggregation may be a factor infection persistence. One mechanism, called depletion aggregation, is driven by physical forces between bacteria polymers. Here we investigated whether the mechanism can actuate aggregating effects of P. aeruginosa exopolysaccharides for (i.e. not surface attached) bacteria, how affects bacterial inter-species interactions. We cells overexpressing Pel...
Abstract The fungal genus Massospora (Zoopagomycota: Entomophthorales) includes more than a dozen obligate, sexually transmissible pathogenic species that infect cicadas (Hemiptera) worldwide. At least two are known to produce psychoactive compounds during infection, which has garnered considerable interest for this enigmatic genus. As with many Entomophthorales, the evolutionary relationships and host associations of spp. not well understood. acquisition M. diceroproctae from Arizona,...