A5018176810- Insect symbiosis and bacterial influences
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Viral Infections and Vectors
- Insects and Parasite Interactions
- Entomopathogenic Microorganisms in Pest Control
- Viral Infections and Outbreaks Research
- Phytoplasmas and Hemiptera pathogens
- Hepatitis C virus research
- Insect behavior and control techniques
- Insect-Plant Interactions and Control
- Nematode management and characterization studies
- Viral gastroenteritis research and epidemiology
- Hepatitis B Virus Studies
- Animal Virus Infections Studies
- Monoclonal and Polyclonal Antibodies Research
- Vector-Borne Animal Diseases
- Influenza Virus Research Studies
- Hepatitis Viruses Studies and Epidemiology
- Neurobiology and Insect Physiology Research
- Plant Parasitism and Resistance
- Insect and Pesticide Research
- Legume Nitrogen Fixing Symbiosis
- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
University of the Ryukyus University Hospital
2020-2025
University of the Ryukyus
2016-2024
National Institute of Infectious Diseases
1984-2020
National Institute of Advanced Industrial Science and Technology
2010-2018
Hokkaido University
1979-2015
University of Tsukuba
2009-2015
Osaka City University
2012
Tokyo University of Science
1991
Significance Cicadas are dependent on the essential bacterial symbionts Sulcia and Hodgkinia . The symbiont genomes extremely streamlined for provisioning of amino acids other nutrients. In some cicada lineages, fragmented into numerous minicircles, which may represent a critical stage genomic erosion close to collapse. What would happen subsequently? Our survey Japanese diversity revealed that while is conserved among all species, majority them have lost instead harbor yeast-like fungal...
Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted the midgut and dense bacterial community hindgut compartment. The genomes gut microbiota encode diverse cellulolytic hemicellulolytic enzymes, but contributions symbionts to degradation remain ambiguous. Our previous studies Nasutitermes spp. documented wood fibers paunch are consistently colonized not only by uncultured members...
SUMMARY The core protein of hepatitis C virus (HCV) is considered to be cleaved from the N terminus large precursor polyprotein by cellular signalase. HCV cDNA encoding was expressed (i) in monkey COS cells a plasmid expression vector driven SRα promoter, and (ii) insect recombinant baculovirus. product had an M r 22000 located cytoplasm. When C-terminal hydrophobic domains were deleted, however, truncated proteins translocated into nucleus. nucleus even when they as fusion with E. coli...
An enzyme-linked immunosorbent assay (ELISA) was developed for serological diagnosis of hepatitis C virus (HCV) infection, using HCV core protein (p22) synthesized by a recombinant baculovirus. Among 58 clinically well-defined chronic non-A, non-B (NANBH) patients, 49 (84.5%) were positive p22 antibody (anti-p22), whereas 42 (72.4%) C100-3 (anti-C100-3), as measured the present nonstructural antigen. Thirty-nine patients (67.2%) had both antibodies. No significant level anti-p22 detected in...
ABSTRACT Here we report a novel clade of secondary endosymbionts associated with insects and other arthropods. Seed bugs the genus Nysius (Hemiptera: Lygaeidae) harbor primary gammaproteobacterial symbiont Schneideria nysicola within pair bacteriomes in abdomen. Our survey species for their facultative bacterial associates consistently yielded type alphaproteobacterial 16S rRNA gene sequence addition to those Wolbachia . Diagnostic PCR 343 individuals representing 24 populations four...
Here, we investigate the endosymbiotic microbiota of Macrosteles leafhoppers M. striifrons and sexnotatus, known as vectors phytopathogenic phytoplasmas. PCR, cloning, sequencing, phylogenetic analyses bacterial 16S rRNA genes identified two obligate endosymbionts, "Candidatus Sulcia muelleri" Nasuia deltocephalinicola," five facultative Wolbachia, Rickettsia, Burkholderia, Diplorickettsia, a novel bacterium belonging to Rickettsiaceae, from leafhoppers. "Ca. deltocephalinicola" exhibited...
Symbiotic bacteria are commonly associated with cells and tissues of diverse animals other organisms, which affect hosts' biology in a variety ways. Most these symbionts present the cytoplasm host maternally transmitted through generations. The paucity paternal symbiont transmission is likely relevant to extremely streamlined sperm structure: head consisting condensed nucleus tail made microtubule bundles, without symbiont-harboring that discarded process spermatogenesis. Here, we report...
The clock mechanism for circatidal rhythm has long been controversial, and its molecular basis is completely unknown. mangrove cricket, Apteronemobius asahinai , shows two rhythms simultaneously in locomotor activity: a producing active inactive phases as well circadian modifying the activity intensity of phases. role gene period ( per ), one key components insects, was investigated A. using RNAi. After injection double-stranded RNA most crickets did not show modulation but persisted without...
Significance Among the most fundamental questions in developmental biology is how novel cell types have emerged metazoan evolution. challenging evolutionary sophisticated symbiotic associations evolved through less intimate interorganismal interactions. These biological issues are crystalized evolution and development of insect’s bacteriocytes specialized for harboring bacteria. Here, we report that a conserved transcription factor Ultrabithorax essential bacteriocyte an insect, thereby...
Timing the acquisition of a beneficial microbe relative to evolutionary history its host can shed light on adaptive impact partnership. Here, we investigated onset and molecular evolution an obligate symbiosis between Cassidinae leaf beetles Candidatus Stammera capleta, γ-proteobacterium. Residing extracellularly within foregut symbiotic organs, upgrades digestive physiology by supplementing plant cell wall-degrading enzymes. We observe that is shared symbiont across tortoise hispine...
Diverse insects and other organisms are associated with microbial symbionts, which often significantly contribute to growth survival of their hosts and/or drastically affect phenotypes in a variety ways. Sodalis glossinidius was first identified as facultative bacterial symbiont tsetse flies, recent studies revealed that Sodalis-allied bacteria encompass diverse ecological niches ranging from free-living through symbionts obligate array insects. Despite potential evolutionary relevance the...
A number of phytophagous stinkbugs (order Heteroptera: infraorder Pentatomomorpha) harbor symbiotic bacteria in a specific midgut region composed numerous crypts. Among the five superfamilies Pentatomomorpha, most members Coreoidea and Lygaeoidea are associated with group genus Burkholderia, called "stinkbug-associated beneficial environmental (SBE)" group, which is not vertically transmitted, but acquired from environment every host generation. recent study reported that, addition to these...
Cassidine leaf beetles are associated with genome-reduced symbiotic bacteria
Stinkbugs of the genus Antestiopsis, so-called antestia bugs or variegated coffee bugs, are notorious pests plants in Africa. We investigated symbiotic bacteria associated with Antestiopsis thunbergii, a major plant pest Rwanda. PCR, cloning, sequencing, and phylogenetic analysis bacterial genes identified four distinct lineages A. thunbergii: gammaproteobacterial gut symbiont symbionts representing genera Sodalis, Spiroplasma, Rickettsia. In situ hybridization showed that densely occupied...
Diverse animals are intimately associated with microbial symbionts. How such host–symbiont associations have evolved is a fundamental biological issue. Recent studies revealed variety of evolutionary relationships, as obligatory, facultative, and free-living, gut bacterial symbiosis within the stinkbug family Pentatomidae, although whole picture remains elusive. Here we investigated comprehensive assembly Japanese pentatomid stinkbugs representing 28 genera, 35 species, 143 populations....
Abstract Mitochondrial genomes can provide valuable information on the biology and evolutionary histories of their host organisms. Here, we present characterize complete coding regions 107 mitochondrial (mitogenomes) cicadas (Insecta: Hemiptera: Auchenorrhyncha: Cicadoidea), representing 31 genera, 61 species, 83 populations. We show that all cicada mitogenomes retain organization gene contents thought to be ancestral in insects, with some variability among clades length a region between...
Human-to-human transmission of PA I38 mutant influenza A(H3N2) viruses with reduced baloxavir susceptibility has been reported in Japan. In December 2019, we detected a E23K A(H1N1)pdm09 virus from child without treatment. The exhibited but remained susceptible to neuraminidase inhibitors. Epidemiological data suggest possible this among humans, although its growth capability relative that the wild-type was reduced. Therefore, monitoring is essential.
Mealybugs (Homoptera: Coccoidea: Pseudococcidae) possess a large bacteriome consisting of number bacteriocytes whose cytoplasm is populated by endosymbiotic bacteria. In many mealybugs the subfamily Pseudococcinae, peculiar configuration has been identified: within bacteriocytes, primary betaproteobacterial endosymbiont Tremblaya princeps endocellularly harbor secondary gammaproteobacterial endosymbionts in nested manner. Meanwhile, some Phenacoccinae are associated only with endosymbiont,...
The maternally-inherited, intracellular bacterium Lariskella (Alphaproteobacteria: Midichloreaceae) has been widely detected in arthropods including true bugs, beetles, a wasp, moth, and pathogen-vectoring fleas ticks. Despite its prevalence, role the biology of hosts unknown. We set out to determine this symbiont leaffooted bug, Leptoglossus zonatus (Hempitera: Coreidae). To examine effects on bug performance reproduction as well possible interactions with bug's obligate nutritional...
SUMMARY Antigenic relationships among 20 strains of haemorrhagic fever with renal syndrome (HFRS) viruses isolated in Korea, China, U.S.S.R., Finland, Japan and U.S.A. were examined rat immune sera, patient eight monoclonal antibodies against the SR-11 strain 10 76–118 strain. Antigen analyses by indirect immunofluorescent antibody adherence haemagglutination tests using polyclonal demonstrated that HRFS may be divided into four serotypes, i.e. Apodemus (Type 1), Rattus 2), Clethrionomys 3)...