A5063813996- Viral Infections and Immunology Research
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Virology and Viral Diseases
- Influenza Virus Research Studies
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Plant Virus Research Studies
- COVID-19 epidemiological studies
- Parvovirus B19 Infection Studies
- Animal Disease Management and Epidemiology
- Viral Infections and Outbreaks Research
- Mosquito-borne diseases and control
- Syphilis Diagnosis and Treatment
- SARS-CoV-2 detection and testing
- COVID-19 Digital Contact Tracing
- RNA regulation and disease
- Whipple's Disease and Interleukins
- COVID-19 Clinical Research Studies
- Infectious Encephalopathies and Encephalitis
Yamagata Prefectural Institute of Public Health
2018-2024
The incidence of modified measles (M-Me), characterized by milder symptoms than those typical (T-Me), has been increasing in Japan. However, the outbreak dominated M-Me cases not thoroughly investigated worldwide. largest importation-related with genotype D8 occurred Yamagata Prefecture, Japan, from March to April 2017. This phenomenon was observed after Japan had achieved elimination 2015. We confirmed 60 detecting genome virus (MeV). Among cases, 38 were and 22 T-Me. Thirty-nine (65.0%)...
Human-to-human transmission of PA I38 mutant influenza A(H3N2) viruses with reduced baloxavir susceptibility has been reported in Japan. In December 2019, we detected a E23K A(H1N1)pdm09 virus from child without treatment. The exhibited but remained susceptible to neuraminidase inhibitors. Epidemiological data suggest possible this among humans, although its growth capability relative that the wild-type was reduced. Therefore, monitoring is essential.
Influenza A(H1N1)pdm09 virus carrying an I38N substitution was detected in untreated teenager Japan. The mutant exhibited reduced susceptibility to baloxavir but remained susceptible neuraminidase inhibitors and showed growth capability. Monitoring antiviral drug of influenza viruses is necessary aid public health planning clinical recommendations.
Abstract We aimed to verify the effectiveness of real-time reverse transcription (rRT) polymerase chain reaction (PCR) for detecting cases modified measles (M-Me) and predicting super-spreader candidates through experience a outbreak dominated by M-Me in Yamagata, Japan, during March–April 2017. applied rRT-PCR specimens from 35 M-Me, nine typical (T-Me) prodromal stage T-Me (P-Me). From among cases, peripheral blood mononuclear cells (PBMC) showed highest positive rate (80.0%), followed...
Abstract Human coronavirus OC43 (HCoV‐OC43) is divided into genotypes A to H based on genetic recombination including the spike ( S ) gene. To investigate longitudinal transition of phylogenetic feature HCoV‐OC43 gene in a community, analysis S1 region was conducted using 208 strains detected Yamagata during 2010 2017 with reference genotype. The sequences were divisible four groups: D. All belonged either group B or In B, 46 (90.2%) out 51 clustered those genotype E (cluster E). D, 28...
Isolation of seasonal coronaviruses, which include human coronavirus (HCoV) OC43, HCoV-HKU1, and HCoV-NL63, from primary cultures is difficult because it requires experienced handling, an exception being HCoV-229E, can be isolated using cell lines such as RD-18S HeLa-ACE2-TMPRSS2. We aimed to isolate CoVs in Yamagata, Japan obtain infective virions useful for further research accelerate fundamental studies on HCoVs SARS-CoV-2. Using modified air-liquid interface (ALI) culture the normal...
At present, 4 human coronaviruses (HCoVs), HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1, that cause upper lower respiratory tract infections are known to be circulating worldwide.In temperate climates, HCoV predominantly occur in the winter early spring (1).Our longitudinal observation of HCoVs Yamagata, Japan supports seasonality these outbreaks (2,3).However, this region surprisingly experienced an outbreak HCoV-OC43 summer 2016.The main purpose paper is describe June 2016 Japan.
Introduction. Although new parechovirus A (PeVA) types, including A3 (PeVA3) and PeVA4, have been reported in this century, there not yet any seroepidemiological studies on PeVA over a period of several decades. Hypothesis/Gap Statement. The authors hypothesize that PeVA3 PeVA4 emerged recently. Aims. aim was to clarify changes the seroprevalence PeVA1, PeVA4. Methodology. Neutralizing antibodies (NT Abs) were measured among residents Yamagata, Japan 1976, 1983, 1985, 1990, 1999 2017....
Public health interventions have played an important role in controlling coronavirus disease 2019 (COVID-19), which is a rapidly spreading infectious disease. To contribute to future COVID-19 countermeasures, we aimed verify the results of countermeasures employed by public centers (PHCs) against first wave Yamagata Prefecture, Japan (Yamagata). Between January and May 2020, 1,253 patients suspected SARS-CoV-2 infection were invited for testing. Simultaneously, based on retrospective contact...
No longitudinal molecular epidemiology of parechovirus A3 (PeV-A3) over a decade is available and PeV-A3-associated myalgia/myositis has been reported only in Japan. Thus, we aimed to clarify the PeV-A3 with major focus on strains detected from cases. We performed sequence phylogenetic analysis for VP1 region Yamagata, Japan, between 2003 2016. The indicated that caused as well variety infectious diseases, ranging mild severe, subjects neonates adults, irrespective genetic cluster or...
Parechovirus A3 was first reported in 2004 and has been recognized as a causative agent of mild severe infections children. Since we an outbreak adult parechovirus A3-associated myalgia Yamagata, Japan 2008, this disease since across Japan, but not yet from other countries.We analysed 19 cases identified Yamagata 2019 to further clarify the epidemiology disease.We performed phylogenetic analyses isolates clinical manifestations genomic clusters.There were two clusters, with cluster 2019B...
Although coxsackievirus A21 (CV-A21) has been associated with an acute respiratory infection (ARI) as well poliomyelitis-like paralysis, reports of CV-A21 detection have quite limited both globally and in Japan. strains were isolated from five sporadic pediatric cases ARI 2019 Yamagata, Neutralizing antibodies (NT Abs) then measured against using sera collected 1976, 1985, 1999, 2009, to clarify the longitudinal epidemiology CV-A21. The total Ab-positive rate each year was 15.2% (35/233),...
Abstract To investigate the antigenic changes in parechovirus 1 (PeVA1), seroepidemiological analyses were performed against Harris strain (Harris), isolated 1956, and PeVA1/Yamagata.JPN/2021‐4785, 2021, using immune sera 207 237 human serum specimens collected 2021 1976, respectively. Although rabbit showed highest neutralization antibody (NT‐Ab) titers immunized viruses at 1:12 800–1:102 400, they cross‐reactive 1:400–1:800. All 62 Yamagata isolates obtained between 2001 (Yamagata...
This method uses a metagenomic approach to analyze the genome sequence of DNA and RNA viruses. Nucleic acids outside viral particles are reduced using nucleases extracted obtain template RNA. Templates converted double-stranded by random amplification, library preparation is performed for analysis on Illumina sequencers. Analysis data with sequences host bacterial origin abundant obtained, allowing multiple samples be analyzed even throughput iSeq100. The protocol was originally folked from...
We identified a child coinfected with influenza B viruses of B/Yamagata and B/Victoria lineages, in whom we analyzed the occurrence genetic reassortment. Plaque purification was performed using throat swab specimen from 9-year-old child, resulting 34 well-isolated plaques. The genomic composition eight gene segments (HA, NA, PB1, PB2, PA, NP, M, NS genes) for each plaque determined at lineage level. Of plaques, 21 (61.8%) had B/Phuket/3073/2013 (B/Yamagata)-like sequences all segments, while...
This protocol aims to determine an nearly complete viral genomic sequence from Illumina sequencing reads which include those originating virus, host cell, and sometimes bacteria. In the primary analysis, raw undergo a filtration process remove non-viral sequences, followed by de novo assembly construct contigs. Subsequently, BLAST program is employed identify full-length genome publicly available shows high homology contig. secondary used as reference align generate consensus sequence,...
This method uses a metagenomic approach to analyze the genome sequence of RNA viruses. Nucleic acids outside viral particles are reduced using nucleases and extracted obtain template RNA. Templates converted double-stranded DNA, library preparation is performed for analysis on Illumina sequencers. Analysis data with sequences host bacterial origin abundant obtained, allowing multiple samples be analyzed even throughput iSeq100. protocol was folked from "Preparation sequencing WTA2 QIAseq FX".
Abstract To clarify the epidemiology of enterovirus D68 (EV‐D68), an rarely identified in 20th century, we performed seroepidemiological analysis against EV‐D68 using sera collected 1976, 1985, 1990, 1999, 2009, and 2019, as well Yamagata isolate (EVD68/Yamagata.JPN/2023‐89), Yamagata, Japan. The neutralizing antibody (Ab)‐positive rates for those under 20 years old were 61.0%, 82.5%, 84.3%, 46.7%, 50.5%, 67.9%, each year, whereas above between 93.4% 99.1%. Generally, geometric mean titers...