A5068867473- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Advanced DC-DC Converters
- Multilevel Inverters and Converters
- Nanopore and Nanochannel Transport Studies
- Muscle activation and electromyography studies
- Vibration Control and Rheological Fluids
- Modular Robots and Swarm Intelligence
- Structural Engineering and Vibration Analysis
- Prosthetics and Rehabilitation Robotics
- Advanced Battery Technologies Research
- Lipid Membrane Structure and Behavior
- Radiomics and Machine Learning in Medical Imaging
- Bacteriophages and microbial interactions
- Seismic Performance and Analysis
- Molecular Junctions and Nanostructures
- Plasma Applications and Diagnostics
- Cerebral Palsy and Movement Disorders
- Engineering Applied Research
- Plasma Diagnostics and Applications
- DNA and Biological Computing
- Micro and Nano Robotics
- Advanced MRI Techniques and Applications
- Biosensors and Analytical Detection
Kent State University
2025
Kyushu Institute of Technology
2022-2025
Tohoku University
2012-2025
University of Tokyo Hospital
2024
Gunma Prefectural College of Health Sciences
2018-2023
Tokyo Institute of Technology
2005-2023
Gunma University Hospital
2019-2023
Ibaraki University
2017-2022
Hitachi (Japan)
2009-2022
Panasonic (Japan)
2019-2020
An amoeba-like molecular robot changes its shape in response to sequence-designed DNA signal molecules.
DNA has the potential to achieve a controllable macromolecular structure, such as hydrogels or droplets formed through liquid-liquid phase separation (LLPS), design of its base sequence can result in programmable interactions. Here, we constructed "DNA droplets" via LLPS sequence-designed nanostructures and controlled their dynamic functions by designing sequences. Specifically, were able adjust temperature required for formation In addition, fusion, fission, Janus-shaped enzymatic...
Abstract Phase‐separated biomolecular droplets are formed in cells to regulate various biological processes. This phenomenon can be applied constructing self‐assembled dynamic molecular systems such as artificial and robots. Recently, programmable phase‐separated called DNA have been reported a possible method construct systems. study reports computational droplet that recognize specific combination of tumor biomarker microRNAs (miRNAs) inputs output logic computing result by physical phase...
Physical properties of DNA droplets formed by liquid–liquid phase separation nanostructures were investigated at a transition temperature. The surface tension was strongly influenced the design sticky ends.
The formation of biomolecular condensates via phase separation relates to various cellular functions. Reconstituting these with designed molecules facilitates the exploration their mechanisms and potential applications. Sequence-designed DNA nanostructures enable investigation how structural design influences condensate construction functional artificial condensates. Despite high designability DNA-based condensates, free that do not assemble into remain a challenge. Combining other...
Photovoltaic (PV) systems having rechargeable batteries are prone to be complex and costly because multiple converters necessary individually regulate a load, PV panel, battery. This paper proposes novel nonisolated multiport (MPCs) integrating bidirectional pulsewidth modulation (PWM) converter phase-shift-switched capacitor (PS-SCC) for standalone systems. A PWM PS-SCC integrated by reducing the total switch count, realizing simplified system circuit. In proposed MPCs, two control freedoms...
Phase separation is a key phenomenon in artificial cell construction. Recent studies have shown that the liquid-liquid phase of designed-DNA nanostructures induces formation liquid-like condensates eventually become hydrogels by lowering solution temperature. As compartmental capsule an essential structure, many focused on lateral lipid vesicles. However, controlling using molecular design approach remains challenging. Here, we present DNA leads to phase-separated capsule-like hydrogels. We...
In recent years, there has been a growing interest in engineering dynamic and autonomous systems with robotic functionalities using biomolecules. Specifically, the ability of molecular motors to convert chemical energy mechanical forces programmability DNA are regarded as promising components for these systems. However, current rely on manual addition external stimuli, limiting potential Here, we show that DNA-based cascade reactions can act controller drives assembly disassembly...
Abstract Small extracellular vesicles (sEVs) play crucial roles in intercellular communication. However, the internalization of individual sEVs by recipient cells has not been directly observed. Here, we examined these mechanisms using state-of-the-art imaging techniques. Single-molecule shows that tumor-derived can be classified into several subtypes. Simultaneous single-sEV particle tracking and observation super-resolution movies membrane invaginations living reveal all sEV subtypes are...
Abstract Self‐assembly of designed nanostructures on a lipid membrane offers powerful approach for enhancing the functionality its surface. Here, environment‐dependent assembly blunt‐ended DNA origami structures phase‐separated lipid‐bilayer consisting liquid‐disordered (Ld) and solid‐ordered (So) phases is reported. Fluorescence microscopy atomic force (AFM) imaging reveal that preferentially bind to So phase. At higher concentrations, origamis form 2D lattices Ld phase through...
Low-quality medical images may influence the accuracy of machine learning process.This study was undertaken to compare image classification among methods, as is a basic aspect clinical inspection.Three types methods were used, which include Support Vector Machine (SVM), Artificial Neural Network (ANN), and Convolution (CNN). To investigate changes in related quality, we constructed single dataset using two different file formats DICOM (Digital Imaging Communications Medicine) JPEG (Joint...
Human-to-human transmission of PA I38 mutant influenza A(H3N2) viruses with reduced baloxavir susceptibility has been reported in Japan. In December 2019, we detected a E23K A(H1N1)pdm09 virus from child without treatment. The exhibited but remained susceptible to neuraminidase inhibitors. Epidemiological data suggest possible this among humans, although its growth capability relative that the wild-type was reduced. Therefore, monitoring is essential.
Recent studies have revealed that liquid-liquid phase separation (LLPS) plays crucial roles in various cellular functions. Droplets formed via LLPS within cells, often referred to as membraneless organelles, serve concentrate specific molecules, thus enhancing biochemical reactions. Artificial systems been utilized construct synthetic cell models, employing a range of molecules. based on DNA nanotechnology are particularly notable for their designable characteristics droplet formation,...
We report a photolithographic method for the shape control of DNA hydrogels based on photo-activated self-assembly Y-shaped nanostructures (Y-motifs). To date, various methods to have been developed enhance functions hydrogel system. However, production shape-controlled formed through without use radical polymerizations has never demonstrated, although such is expected be applied shape-control encapsulating sensitive biomolecules, as proteins. In this study, we used linker initiate Y-motifs,...
Regulating dynamic behavior of the designed molecular structures provides a foundation for construction functional devices. DNA nanotechnology allows conformational changes in two‐dimensional and three‐dimensional origami nanostructures by introducing flexibility between faces structures. However, transformations wireframe origami, composed solely vertices edges, remain challenging due to vertex‐specific flexibility. We report capable vertex‐protruding transformation open‐ closed‐form with...
In histology, immunostaining of biological samples is a gold standard for studying cellular processes, such as the expression cell surface markers or uptake proteins and drug molecules. Immuno-gold labeling commonly used technique to achieve nanometer spatial resolution, but simultaneous visualization multiple antigens in parallel an unresolved challenge. Herein, we demonstrate DNA nanotechnology-based approach label transmission electron microscopy images tissue sections with high contrast...
Interleaved converters are a typical solution to enhance current capacity and reduce ripples of an input or output port. Conventional interleaved LLC resonant need additional switches passive components with feedback control loop for active balancing, increasing the circuit complexity cost. This paper proposes novel three-phase converter automatic capacitive balancing capability. Two flying capacitors added conventional in order realize not only but also reduced switch voltage stresses...
An isothermal amplification circuit for specific DNA molecules was implemented in giant unilamellar vesicles. Using this circuit, over 5000-fold of output DNAs achieved, and the behaviour depended on concentration input signal a cell-sized compartment. Moreover, initiation by photo-stimulation demonstrated.
Abstract DNA is an excellent material for constructing self‐assembled nano/microstructures. Owing to the widespread use of as a building block in laboratories and industry, it desirable increase efficiency all steps involved producing structures. One bottlenecks purification required separate excess components from target This paper describes method based on fractionation by water‐in‐water (W/W) droplets composed phase‐separated dextran‐rich polyethylene glycol (PEG)‐rich continuous phase....
We have developed a prototype for walkingassistance apparatus that serves as next-generation vehicle or movable neuro-rehabilitation training appliance the elderly motor palsy patients. Our uses novel spatial parallel link mechanism with weight-bearing lift. The flat steps of move in ground; supports complete leg alignment, including soles feet, and assists walking behavior at ankle, knee, hip joints simultaneously. To estimate phase each user, pressure sensors were attached under thenar...