A5108830088- Influenza Virus Research Studies
- Respiratory viral infections research
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Data-Driven Disease Surveillance
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Viral Infections and Immunology Research
- COVID-19 epidemiological studies
- SARS-CoV-2 detection and testing
- vaccines and immunoinformatics approaches
- Virology and Viral Diseases
- COVID-19 Clinical Research Studies
- Protein Structure and Dynamics
- Hepatitis Viruses Studies and Epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Evolution and Genetic Dynamics
- Veterinary medicine and infectious diseases
- Survey Methodology and Nonresponse
- Hepatitis B Virus Studies
- Infectious Encephalopathies and Encephalitis
- Virus-based gene therapy research
- Polysaccharides Composition and Applications
National Institute of Infectious Diseases
2016-2025
The Francis Crick Institute
2024
National Center for Immunization and Respiratory Diseases
2024
Centers for Disease Control and Prevention
2024
University of Washington
2024
National Institutes of Health
2010-2024
Fred Hutch Cancer Center
2024
Icahn School of Medicine at Mount Sinai
2024
Howard Hughes Medical Institute
2024
Brotman Baty Institute
2024
The hemagglutinin (HA) of influenza A viruses has been classified into sixteen distinct subtypes (H1–H16) to date. HA are principally defined as serotypes determined by neutralization or hemagglutination inhibition tests using polyclonal antisera the respective subtypes, which have little cross-reactivity other subtypes. Thus, it is generally believed that neutralizing antibodies not broadly cross-reactive among In this study, we generated a novel monoclonal antibody (MAb) specific HA,...
ABSTRACT Highly pathogenic avian influenza viruses with H5 and H7 hemagglutinin (HA) subtypes evolve from low-pathogenic precursors through the acquisition of multiple basic amino acid residues at HA cleavage site. Although this mechanism has been observed to occur naturally only in these subtypes, little is known about genetic basis for polybasic Here we show that consecutive adenine a stem-loop structure, which are frequently found viral RNA region encoding acids around site isolated...
In January 2019, two influenza A(H3N2) viruses carrying an I38T substitution in the polymerase acidic subunit (PA), which confers reduced susceptibility to baloxavir, were detected from epidemiologically unrelated hospitalised children Japan. The exhibited baloxavir but susceptible neuraminidase inhibitors. Only one of had been treated with baloxavir. An epidemiological analysis suggests possible transmission PA mutant virus among humans.
The novel cap-dependent endonuclease inhibitor baloxavir marboxil was approved for the treatment of influenza virus infection in Japan February 2018. Two A(H3N2) viruses carrying an I38T substitution polymerase acidic subunit (PA) were detected baloxavir-treated children December This mutation is known to confer reduced susceptibility baloxavir, and two mutant exhibited 76- 120-fold baloxavir.
In 2019, influenza A(H3N2) viruses carrying an I38T substitution in the polymerase acidic gene, which confers reduced susceptibility to baloxavir, were detected Japan infant without baloxavir exposure and a baloxavir-treated sibling. These viruses' whole-genome sequences identical, indicating human-to-human transmission. Influenza virus isolates should be monitored for susceptibility.
The novel cap-dependent endonuclease inhibitor baloxavir marboxil was approved for the treatment of influenza A and B virus infections in February 2018 Japan. Because need to monitor viruses reduced susceptibility this drug, we used two cell-based screening systems - a conventional plaque reduction assay focus evaluate baloxavir. First, generated reference possessing an I38T substitution polymerase acidic subunit (PA), which is known be associated with baloxavir, demonstrated validity our...
Several enzyme-linked immunosorbent assays (ELISAs) for the detection of filovirus-specific antibodies have been developed. However, diagnostic methods to distinguish specific respective species filoviruses, which provide basis serological classification, are not readily available. We established an ELISA using His-tagged secreted forms transmembrane glycoproteins (GPs) five different Ebola virus (EBOV) and one Marburg (MARV) strain as antigens filovirus species-specific antibodies. The...
Seasonal influenza virus A/H3N2 is a major cause of death globally. Vaccination remains the most effective preventative. Rapid mutation hemagglutinin allows viruses to escape adaptive immunity. This antigenic drift necessitates regular vaccine updates. Effective strains need represent H3N2 populations circulating one year after strain selection. Experts select based on experimental measurements and predictions made by models from sequences. We developed novel forecasting framework that...
Influenza viruses continually evolve new antigenic variants, through mutations in epitopes of their major surface proteins, hemagglutinin (HA) and neuraminidase (NA). Antigenic drift potentiates the reinfection previously infected individuals, but contribution this process to variability annual epidemics is not well understood. Here, we link influenza A(H3N2) virus evolution regional epidemic dynamics United States during 1997—2019. We integrate phenotypic measures HA sequence-based NA...
Influenza viruses continually evolve new antigenic variants, through mutations in epitopes of their major surface proteins, hemagglutinin (HA) and neuraminidase (NA). Antigenic drift potentiates the reinfection previously infected individuals, but contribution this process to variability annual epidemics is not well understood. Here, we link influenza A(H3N2) virus evolution regional epidemic dynamics United States during 1997—2019. We integrate phenotypic measures HA sequence-based NA...
Marburg virus (MARV) and Ebola (EBOV) cause severe hemorrhagic fever in primates. Earlier studies demonstrated that antibodies to particular epitopes on the glycoprotein (GP) of EBOV enhanced infectivity vitro.To investigate this antibody-dependent enhancement (ADE) MARV infection, we produced mouse antisera monoclonal (mAbs) GPs strains Angola Musoke.The vesicular stomatitis pseudotyped with GP K562 cells was significantly presence antisera, whereas only minimal ADE activity seen Musoke...
SUMMARY Background The immune profile against SARS-CoV-2 has dramatically diversified due to a complex combination of exposure vaccines and infection by various lineages/variants, likely generating heterogeneity in protective immunity given population. To further complicate this, the Omicron variant, with numerous spike mutations, emerged. These circumstances have created need assess potential evasion individuals histories. Methods neutralization susceptibility variants including their...
Abstract To monitor oseltamivir-resistant influenza viruses A (H1N1) (ORVs) with H275Y in neuraminidase (NA) Japan during 2 seasons, we analyzed 3,216 clinical samples by NA sequencing and/or inhibition assay. The total frequency of ORVs was 2.6% (45/1,734) the 2007–08 season and 99.7% (1,477/1,482) 2008–09 season, indicating a marked increase 1 season. gene fell into distinct lineages D354G substitution, whereas that lineage. assay M2 showed almost all were sensitive to zanamivir...
The glycoproteins (GPs) of filoviruses are responsible for virus entry into cells. It is known that GP interacts with cellular C-type lectins attachment to Since primary target cells express lectins, lectin-mediated thought be a possible determinant tropism and pathogenesis. We compared the efficiency between Marburg strains Angola Musoke by using vesicular stomatitis (VSV) pseudotype system. VSV pseudotyped (VSV-Angola) infected K562 expressing lectin, human macrophage galactose-type lectin...
An influenza A(H1N1)pdm09 virus carrying a G147R substitution in combination with an H275Y the neuraminidase protein, which confers cross-resistance to oseltamivir and peramivir, was detected from immunocompromised inpatient Japan, March 2016. This dual H275Y/G147R mutant exhibited enhanced both drugs compared single reduced susceptibility zanamivir, although it showed normal inhibition by laninamivir.
Human-to-human transmission of PA I38 mutant influenza A(H3N2) viruses with reduced baloxavir susceptibility has been reported in Japan. In December 2019, we detected a E23K A(H1N1)pdm09 virus from child without treatment. The exhibited but remained susceptible to neuraminidase inhibitors. Epidemiological data suggest possible this among humans, although its growth capability relative that the wild-type was reduced. Therefore, monitoring is essential.
The vaccine S-268019-b is a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S)-protein consisting of full-length recombinant SARS-CoV-2 S-protein (S-910823) as antigen, mixed with the squalene-based adjuvant A-910823. current study evaluated immunogenicity using various doses S-910823 and its efficacy against challenge in cynomolgus monkeys. different combined A-910823 were intramuscularly administered twice at 3-week interval. Two weeks after second dosing,...