A5082643440- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Clostridium difficile and Clostridium perfringens research
- Salmonella and Campylobacter epidemiology
- Gut microbiota and health
- SARS-CoV-2 and COVID-19 Research
- Inflammatory Bowel Disease
- Listeria monocytogenes in Food Safety
- Essential Oils and Antimicrobial Activity
- Immune Cell Function and Interaction
- Bacterial Genetics and Biotechnology
- Brucella: diagnosis, epidemiology, treatment
- Enterobacteriaceae and Cronobacter Research
- Aquaculture disease management and microbiota
- Vaccine Coverage and Hesitancy
- Food Safety and Hygiene
- Medical Device Sterilization and Disinfection
- Bacterial Infections and Vaccines
- Hepatitis Viruses Studies and Epidemiology
- Pneumonia and Respiratory Infections
- Transgenic Plants and Applications
- Allergic Rhinitis and Sensitization
- RNA modifications and cancer
University of Chile
2015-2025
Universidad Bernardo O'Higgins
2022-2024
University of Concepción
2023-2024
Millennium Institute on Immunology and Immunotherapy
2019-2024
Institute of Biomedical Science
2015
Faculty (United Kingdom)
2015
Listeria monocytogenes is a pathogen transmitted through food that can cause severe infections in high-risk groups such as pregnant women, elderly, young children and immunocompromised individuals. It ubiquitous bacterium survive harsh conditions, dry environments, at low temperatures, brine conditions pH values. also has the capacity to form biofilms, which makes it particularly successful even colonizing surfaces within processing plants. This study analyzed presence of L. ready-to-eat...
Healthcare-associated infections (HAIs) have a major impact on public health worldwide. Particularly, hospital surfaces contaminated with bacterial pathogens are often the origin of both sporadic cases and outbreaks HAIs. It has been demonstrated that copper reduce microbial burden high touch in environment. Here we report antimicrobial characterization novel composite coating embedded particles, named Copper Armour™.The Armour™ bactericidal activity was evaluated vitro assays against...
Shiga Toxin-producing Escherichia coli (STEC) are a group of foodborne pathogens associated with diarrhea, dysentery, hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). toxins the major virulence factor these pathogens, however adhesion colonization to human intestine is required for STEC pathogenesis. A subset strains carry Locus Enterocyte Effacement (LEE) pathogenicity island (PAI), which encodes genes that mediate intestine. While LEE-positive have traditionally been disease,...
Allergic asthma has emerged as a prevalent allergic disease worldwide, affecting most prominently both young individuals and lower-income populations in developing developed countries. To devise effective curative immunotherapy, it is crucial to comprehend the intricate nature of this condition, characterized by an immune response imbalance that favors proinflammatory profile orchestrated diverse subsets cells. Although involvement Natural Killer T (NKT) cells pathology frequently implied,...
Shiga toxin-producing Escherichia coli (STEC) are foodborne pathogens causing severe gastroenteritis, which may lead to hemolytic uremic syndrome. The Locus of Enterocyte Effacement (LEE), a Pathogenicity Island (PAI), is major determinant intestinal epithelium attachment group STEC strains; however, the virulence repertoire strains lacking LEE, has not been fully characterized. incidence LEE-negative increased in several countries, highlighting relevance their study. In order gain insights...
ABSTRACT Shiga-toxin producing Escherichia coli (STEC) is the etiologic agent of acute diarrhea, dysentery, and hemolytic-uremic syndrome (HUS). There no approved vaccine for STEC infection in humans, antibiotic use contraindicated, as it promotes Shiga toxin production. In order to identify STEC-associated antigens immunogenic proteins, outer membrane proteins (OMPs) were extracted from O26:H11, O103, O113:H21, O157:H7 strains, commensal E. strain HS was used a control. SDS-PAGE,...
Background: Healthcare-associated infections (HAIs) significantly increase morbidity, mortality, and healthcare costs. Among HAIs, catheter-associated are particularly prevalent due to the susceptibility of catheters microbial contamination biofilm formation, especially with prolonged use. Biofilms act as infection reservoirs, complicating treatment often requiring catheter removal, thus extending hospital stays increasing Recent technological advances in design have focused on integrating...
Listeria monocytogenes causes severe diseases in humans, including febrile gastroenteritis and systemic infections that has a high mortality despite antibiotic treatment. This pathogen may cause massive outbreaks associated to the consumption of contaminated food products, which highlight its importance public health. In last decade, L. emerged as foodborne major Chile. A previous work showed Chile during 2008 2009, serotypes 1/2a, 1/2b 4b were most frequently identified clinical strains....
is a human pathogen, which transmitted by the consumption of contaminated food or water.
Inflammatory bowel diseases (IBDs), which include ulcerative colitis (UC) and Crohn's disease (CD), cause chronic inflammation of the gut, affecting millions people worldwide. IBDs have been frequently associated with an alteration gut microbiota, termed dysbiosis, is generally characterized by increase in abundance Proteobacteria such as Escherichia coli, a decrease Firmicutes Faecalibacterium prausnitzii (an indicator healthy colonic microbiota). The mechanisms behind development dysbiosis...
Enterotoxigenic Escherichia coli (ETEC), a leading cause of acute diarrhea, colonizes the intestine by means adhesins. However, 15 to 50% clinical isolates are negative for known adhesins, making it difficult identify antigens broad-coverage vaccines. The ETEC strain 1766a, obtained from child with watery diarrhea in Chile, harbors colonization factor CS23 but is other One clone, derived an 1766a genomic library (clone G10), did not produce yet was capable adhering Caco-2 cells. goal this...
Abstract Shiga toxin-producing Escherichia coli (STEC) cause diarrhea and dysentery, which may progress to hemolytic uremic syndrome (HUS). Vaccination has been proposed as a preventive approach against STEC infection; however, there is no vaccine for humans those used in animals reduce but do not eliminate the intestinal colonization of STEC. The OmpT, Cah Hes proteins are widely distributed among clinical strains recognized by serum IgG IgA patients with HUS. Here, we develop formulation...
Shiga toxin-producing Escherichia coli (STEC) causes outbreaks and sporadic cases of gastroenteritis. STEC O157:H7 is the most clinically relevant serotype in world. The major virulence determinants are toxins locus enterocyte effacement. However, several accessory factors, mainly outer membrane proteins (OMPs) that interact with host cells may contribute to this pathogen. Previously, elongation factor thermo unstable (EF-Tu), l-asparaginase II OmpT were identified as antigens OMP extracts...
Shigellosis is an enteric infectious disease in which antibiotic treatment effective, shortening the duration of symptoms and reducing excretion pathogen into environment. Shigella spp., etiologic agent, are considered emerging pathogens with a high public health impact due to increase global spread multidrug-resistant (MDR) strains. Since resistance phenotype varies worldwide, we present overview phenotypes associated genetic determinants 349 Chilean S. sonnei strains isolated during...
Aerial and respiratory tract-associated bacterial diversity has been scarcely studied in broiler production systems. This study examined the relationship between environmental air birds’ microbiome, considering a longitudinal sampling. Total viable bacteria coliforms were quantified, 16S rRNA gene was sequenced from tracheal samples obtained through novelty protocol. Air results showed decrease over time. However, at week 3, we reported an increase (from 143 to 474 CFUc/m3) associated with...
Shiga toxin-producing Escherichia coli strains LAA-positive are important cause of human infection. The capability to adhere epithelial cells is a key virulence trait, and genes codified in LAA pathogenicity island could be involved the adhesion during pathogenesis STEC strains. Thus, our objectives were compare hes-negative hes-positive their adherence (HEp-2) evaluate expression levels hes, iha, tpsA bacteria adhered non-adhered HEp-2 cells. These encoded LAA, factors that participate...
Shiga toxin-producing
Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen that causes gastroenteritis and Hemolytic Uremic Syndrome. Cattle are the main animal reservoir, excreting bacteria in their feces contaminating environment. In addition, meat can be contaminated by releasing intestinal content during slaughtering. Here, we evaluated safety immunogenicity of vaccine candidate against STEC was formulated with two chimeric proteins (Chi1 Chi2), which contain epitopes OmpT, Cah Hes proteins....