More than 400 serotypes of Shiga toxin-producing Escherichia coli (STEC) have been implicated in outbreaks and sporadic human diseases. In recent years STEC strains belonging to serogroup O178 commonly isolated from cattle food bovine origin South America Europe. order explore the significance these as potential pathogens, 74 German Argentinean E. animals, humans were characterized phenotypically investigated for their serotypes, stx-genotypes forty-three virulence-associated markers by a...

The hemolytic uremic syndrome (HUS) whose main causative agent is enterohemorrhagic Escherichia coli (EHEC) O157:H7 a disease that mainly affects children under 5 years of age. Argentina the country with highest incidence HUS in world. Cattle are major reservoir and source infection E. O157:H7. To date, epidemiological factors contribute to its prevalence poorly understood. Single nucleotide polymorphism (SNP) typing has helped define nine clades clade 8 strains were associated most cases...

Shiga toxin-producing Escherichia coli (STEC), responsible for the hemolytic uremic syndrome, is an endemic pathogen in Argentina. We studied prevalence of STEC fecal samples from cats and dogs Buenos Aires city suburbs. Cultures were used screening stx1/stx2 rfbO157 by multiplex PCR. All E. coli-positive colonies these genes further characterized eae gene serotypes. In dogs, 17 (3.7%), 19 (4.2%) 34 (7.5%) positive stx2, stx1 rfb, respectively. cats, six (4.0%) three (2.0%) four (2.7%)...

For years, the Bacillus genus has encompassed a heterogeneous group of individuals whose main common trait was their ability to sporulate in presence oxygen. This criterion been revised, resulting reclassification several species into new genera and redefinition characteristics members this taxon. Currently, are grouped Subtilis clade Cereus clade. The former, called sensu stricto, initially composed B. subtilis, licheniformis, pumilus, amyloliquefaciens, subsequently incorporated related...

Consumption of raw/undercooked ground beef is the most common route transmission Shiga toxin-producing E. coli (STEC). The aim study was to determine STEC contamination level samples collected in 36 markets different socioeconomic strata Buenos Aires, Argentina, and characterization isolated strains. Ninety-one out 252 (36.1%) were stx+ . Fifty-seven strains recovered. Eleven belonged O157 serogroup, 46 non-O157 serogroups. Virulence markers 57 stx 1, 5.3% (3/57); 2, 86.0% (49/57); 1/ 8.8%...

Southern right whales Eubalaena australis from Peninsula Valdés, Argentina, show wounds produced by kelp gulls Larus dominicanus that feed on the whales' dorsal skin and blubber. During 2013 whale season, several calves were reported showing gull injuries with a swollen area surrounded rhomboid-shaped raised edges. Samples 9 taken in order to establish etiology of these wounds; 2 (one living, one dead) showed gull-inflicted dead calf histologically characterized presence dermal congestion,...

Introduction: Bovine mastitis is a frequent cause of economic loss in dairy herds. Coagulase-negative staphylococci (CoNS) are increasing importance as causeof bovine intramammary infection (IMI) throughout the world recent years. CoNShave been isolated from milk samples collected cows with clinical andsubclinical several countries. Identification pathogensis important when selection appropriate antimicrobial therapy.Methodology: A total 93 strains Staphylococcusspp Argentina during...

Shigatoxigenic Escherichia coli (STEC) strains are worldwide zoonotic pathogen responsible for different cases of human disease including hemolytic uremic syndrome (HUS). Transmission STEC to humans occurs through the consumption food and water contaminated by faeces carriers person-to-person contact. The objective this study was two-fold: (1) investigate whether synanthropic rodents possible reservoirs in urban area (2) a particular genus out rodent is principal carrier STEC. One hundred...

Introduction: Staphylococcus aureus and coagulase-negative staphylococci (CNS) are frequently isolated from cows with mastitis. A main virulence factor of CNS is the ability to adhere form biofilms. The intercellular gene cluster adhesion (ica) operon one involved in biofilm production although ica-independent factors also involved. Previous reports based on results S. epidermidis suggested that ica highly conserved between species, but this detection decreases other producers. In study we...

Introduction: Hemolytic uremic syndrome (HUS) is distributed worldwide. In Argentina, more than 450 cases of HUS, mostly sporadic, are reported annually. The main serotype isolated O157:H7, and among non-O157 STEC, O145:NM the most frequent strain. We studied relationship companion animals living in contact with a child sporadic as carriers Shiga toxin-producing Escherichia coli (STEC) strains. Methodology: Duplicate rectal swab samples were taken weekly from household cat dog at home...

Introduction: Shiga toxin-producing Escherichia coli (STEC) infections are the leading cause of hemolytic uremic syndrome (HUS). STEC is most common acute kidney disease, responsible for 20% renal transplants in Argentina. Methodology: In 2007, an epidemiological survey was conducted among 883 students from fifth and sixth years elementary education public schools San Martin City, Buenos Aires, Degree exposure to known risk factors previously detected region as primary causes evaluated. Risk...

Enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. (STEC) are pathovars of that impact human health by causing childhood diseases. In this work, 118 synanthropic rodents the genus Rattus from Buenos Aires, Argentina were evaluated as EPEC STEC carriers. Rectal swab samples captured animals conventional PCR to detect presence eae, stx1, stx2, rfbO157 genes. Twenty-one isolates obtained (17 seven four same animal). All tested negative for bfpA gene. One isolate carried iha...

BACKGROUND: Enterotoxaemia produced by Clostridium perfringens A, C and D is an important cause of mortality in young llamas. There no data on antibody responses following vaccination with epsilon toxin. METHODOLOGY: Twenty-six L. glama crias were divided into four groups which vaccinated a commercial vaccine (Mancha Gangrena Enterotoxemia, Instituto Rosembusch Sociedad Anónima, Argentina) days 0, 21 42 or left as unvaccinated controls. An indirect ELISA was compared the mouse neutralization...

Atypical enteropathogenic Escherichia coli (aEPEC) is comprised of a large heterogeneous group strains and serotypes that carry the intimin gene (eae) but no other EPEC virulence factors. In previous study, we examined few aEPEC O157:H16 serotype from U.S. France found these to be nearly homologous, speculated same strain had been disseminated or perhaps they are part clonal exists worldwide. To test hypothesis, additional 45 isolated various sources 4 countries determined although there...

Shiga toxin-producing

Diarrheagenic Escherichia coli (DEC) pathovars impact childhood health. The southern region of Argentina shows the highest incidence hemolytic uremic syndrome (HUS) in children country. big island Tierra del Fuego (TDF) registered an five cases/100,000 inhabitants HUS 2019. This work aimed to establish prevalence STEC, EPEC, and EAEC lambs slaughtered abattoirs from TDF as well characterize phenotypes genotypes isolated pathogens. was 26.6% for stx+, 5.7% eae+, 0.27% aagR+/aaiC+. Twelve STEC...

Abstract Cathepsin L proteinases (CL1 and CL2), the major components of Fasciola hepatica excretion/secretion products (E/S) are considered potential antigens a vaccine against fascioliasis. The humoral response elicited by CL1 CL2 in rats either immunized with enzymes or infected F. has been analysed, examining specific IgE IgG subclass dynamics. experiment was continued for 10 weeks peripheral blood eosinophilia also determined. Infected presented peaks at 3 7 post-infection, while those...