A5074182580- Biochemical and Molecular Research
- HIV/AIDS drug development and treatment
- Synthesis and Characterization of Heterocyclic Compounds
- Carbohydrate Chemistry and Synthesis
- Cytomegalovirus and herpesvirus research
- DNA and Nucleic Acid Chemistry
- Polyamine Metabolism and Applications
- Adenosine and Purinergic Signaling
- Amino Acid Enzymes and Metabolism
- Virus-based gene therapy research
- Chronic Lymphocytic Leukemia Research
- Fluorine in Organic Chemistry
- Cancer therapeutics and mechanisms
- Herpesvirus Infections and Treatments
- Click Chemistry and Applications
- Synthesis and Reactions of Organic Compounds
- Porphyrin Metabolism and Disorders
- Synthesis and Biological Evaluation
- Synthesis and biological activity
- HIV Research and Treatment
- Hepatitis C virus research
- Peptidase Inhibition and Analysis
- Pneumocystis jirovecii pneumonia detection and treatment
- Quinazolinone synthesis and applications
- Synthesis of Organic Compounds
Southern Research Institute
2006-2020
Moffitt Cancer Center
2007-2009
Pennsylvania State University
2001-2009
Cornell University
1998-2009
University of South Florida
2007-2009
Penn State Milton S. Hershey Medical Center
2009
University of Alabama
2008
Florida College
2007
National Institutes of Health
1999
University of Alabama at Birmingham
1991
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFluorescent modification of adenosine-containing coenzymes. Biological activities and spectroscopic propertiesJohn A. Secrist, III, Jorge R. Barrio, Nelson J. Leonard, Gregorio WeberCite this: Biochemistry 1972, 11, 19, 3499–3506Publication Date (Print):September 12, 1972Publication History Published online1 May 2002Published inissue 12 September 1972https://pubs.acs.org/doi/10.1021/bi00769a001https://doi.org/10.1021/bi00769a001research-articleACS...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and biological activity of 2'-deoxy-4'-thio pyrimidine nucleosidesJohn A. Secrist III, Kamal N. Tiwari, James M. Riordan, John MontgomeryCite this: J. Med. Chem. 1991, 34, 8, 2361–2366Publication Date (Print):August 1, 1991Publication History Published online1 May 2002Published inissue 1 August 1991https://pubs.acs.org/doi/10.1021/jm00112a007https://doi.org/10.1021/jm00112a007research-articleACS PublicationsRequest reuse...
A new, highly fluorescent adenosine triphophate (ATP) analog, 1,N(6) ethenoadenosine triphosphate, has been synthesized. Its fluorescence properties, including the long lifetime and possibility of detection at very low concentrations, in conjunction with its activity representative enzyme systems here reported, make it a valuable probe enzymic mechanism structure.
Competitive inhibitors of the salvage pathway enzyme purine-nucleoside phosphorylase (purine-nucleoside:orthophosphate ribosyltransferase, EC 2.4.2.1) have been designed by using three-dimensional structure as determined x-ray crystallography. The process was an iterative one that utilized interactive computer graphics, Monte Carlo-based conformational searching, energy minimization, and proposed compounds were synthesized tested in vitro assay. Among are most potent competitive purine...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and biological activity of 2'-fluoro-2-halo derivatives 9-.beta.-D-arabinofuranosyladenineJohn A. Montgomery, Anita T. Shortnacy-Fowler, Sarah D. Clayton, James M. Riordan, John Secrist IIICite this: J. Med. Chem. 1992, 35, 2, 397–401Publication Date (Print):January 1, 1992Publication History Published online1 May 2002Published inissue 1 January...
Carbovir (the carbocyclic analog of 2'-3'-didehydro-2',3'-dideoxyguanosine) is a potent inhibitor human immunodeficiency virus type 1 (HIV-1) replication. Assays were developed to assess the mechanism inhibition by 5'-triphosphate carbovir HIV-1 reverse transcriptase using either RNA or DNA templates that contain all four natural nucleotides. Carbovir-TP was template with Ki values similar observed AZT-TP, ddGTP, and ddTTP. The kinetic constants for incorporation these nucleotide analogs...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure-based design of inhibitors purine nucleoside phosphorylase. 1. 9-(Arylmethyl) derivatives 9-deazaguanineJohn A. Montgomery, Shri Niwas, Jerry D. Rose, John Secrist III, Y. Sudhakar Babu, Charles E. Bugg, Mark Erion, Wayne C. Guida, and Steven EalickCite this: J. Med. Chem. 1993, 36, 1, 55–69Publication Date (Print):January 1993Publication History Published online1 May 2002Published inissue 1 January...
Nicotinamide 1,N(6)-ethenoadenine dinucleotide, a fluorescent analog of the coenzyme nicotinamide adenine has been synthesized by reaction chloroacetaldehyde with coenzyme. The technical fluorescence emission maximum is 410 nm, upon excitation at 300 nm. Its yield about 8% that 5'-phosphate, and its lifteime shorter. Upon hydrolysis modified Neurospora crassa NADase or phosphodiesterase I room temperature, intensity was increased 10-fold, corresponding to separation ethenoadenine rings....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTotal synthesis of erythromycins. 3. Stereoselective routes to intermediates corresponding C(1) C(9) and C(10) C(13) fragments erythronolide BE. J. Corey, Eugene Trybulski, Lawrence S. Melvin Jr., K. C. Nicolaou, John A. Secrist, Robert Lett, Peter W. Sheldrake, R. Falck, Daniel Brunelle, Cite this: Am. Chem. Soc. 1978, 100, 14, 4618–4620Publication Date (Print):July 1, 1978Publication History Published online1 May 2002Published inissue 1 July...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAmine hydrochlorides by reduction in the presence of chloroformJohn A. Secrist III and Marshall W. LogueCite this: J. Org. Chem. 1972, 37, 2, 335–336Publication Date (Print):January 1, 1972Publication History Published online1 May 2002Published inissue 1 January 1972https://pubs.acs.org/doi/10.1021/jo00967a042https://doi.org/10.1021/jo00967a042research-articleACS PublicationsRequest reuse permissionsArticle Views958Altmetric-Citations89LEARN ABOUT...
We have developed a new strategy for the gene therapy of cancer based on activation purine nucleoside analogs by transduced E. coli phosphorylase (PNP, E.C. 2.4.2.1). The approach is designed to generate antimetabolites intracellularly that would be too toxic systemic administration. To determine whether this could used kill tumor cells without host toxicity, nude mice bearing human malignant D54MG glioma tumors expressing PNP (D54–PNP) were treated with either...
Abstract In the past few years interest in synthesis and biological properties of acyclic nucleosides has been generated based largely upon development acyclovir [9-[(2-hydroxyethoxy)methyll guanine, 1a] as an antiviral agent for treatment certain herpesvirus infections. The literature area covers a variety different heterocycles, side chains, spans research that is strictly synthetic to biological. Two compounds, 9-[(2-hydroxy-l-(hydroxymethyl)ethoxy)methyl] guanine (1b)...
The synthesis of a highly fluorescent analog adenosine 3',5'-monophosphate, namely, 1,N(6)-ethenoadenosine has provided powerful probe for systems involving 3',5'-monophosphate. potential utility this is indicated by its long lifetime, detectability at low concentration, and relatively wavelength excitation (300 nanometers). In protein kinase it acceptable substitute
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSearch for New Purine- and Ribose-Modified Adenosine Analogs as Selective Agonists Antagonists at ReceptorsSuhaib M. Siddiqi, Kenneth A. Jacobson, John L. Esker, Mark E. Olah, Xiao-duo Ji, Neli Melman, Kamal N. Tiwari, Secrist III, Stewart W. Schneller, Cite this: J. Med. Chem. 1995, 38, 7, 1174–1188Publication Date (Print):March 1, 1995Publication History Published online1 May 2002Published inissue 1 March...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and anti-HIV activity of 4'-thio-2',3'-dideoxynucleosidesJohn A. Secrist III, Robert M. Riggs, Kamal N. Tiwari, John MontgomeryCite this: J. Med. Chem. 1992, 35, 3, 533–538Publication Date (Print):February 1, 1992Publication History Published online1 May 2002Published inissue 1 February 1992https://pubs.acs.org/doi/10.1021/jm00081a015https://doi.org/10.1021/jm00081a015research-articleACS PublicationsRequest reuse permissionsArticle...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConstruction of long-chain carbohydrates. Synthesis and chemistry a galactose 6-phosphoraneJohn A. Secrist III Shang-Ren WuCite this: J. Org. Chem. 1979, 44, 9, 1434–1438Publication Date (Print):April 1, 1979Publication History Published online1 May 2002Published inissue 1 April 1979https://pubs.acs.org/doi/10.1021/jo01323a016https://doi.org/10.1021/jo01323a016research-articleACS PublicationsRequest reuse permissionsArticle...