A5087219775- Cholesterol and Lipid Metabolism
- Fatty Acid Research and Health
- Atherosclerosis and Cardiovascular Diseases
- MicroRNA in disease regulation
- Cancer, Lipids, and Metabolism
- Antioxidant Activity and Oxidative Stress
- Liver Disease Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Phytochemicals and Antioxidant Activities
- RNA Research and Splicing
- Axon Guidance and Neuronal Signaling
- Cardiovascular Disease and Adiposity
- Aquaculture Nutrition and Growth
- Steroid Chemistry and Biochemistry
- Circular RNAs in diseases
- Immune cells in cancer
- Apelin-related biomedical research
- Diet, Metabolism, and Disease
- Galectins and Cancer Biology
- Lipoproteins and Cardiovascular Health
- Lipid metabolism and biosynthesis
- RNA modifications and cancer
- Adipokines, Inflammation, and Metabolic Diseases
- Phagocytosis and Immune Regulation
- Natural Antidiabetic Agents Studies
New York University
2018-2024
Gilead Sciences (United States)
2022-2024
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2022
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2019-2022
NYU Langone Health
2018-2019
Columbia University Irving Medical Center
2019
Universidade de São Paulo
2012-2017
University of Ottawa
2017
National Research Council Canada
2017
Columbia University
2017
Rationale: Regression of atherosclerosis is an important clinical goal; however, the pathways that mediate resolution atherosclerotic inflammation and reversal plaques are poorly understood. Regulatory T cells (Tregs) have been shown to be atheroprotective, yet numbers these immunosuppressive decrease with disease progression, whether they contribute regression not known. Objective: We investigated roles Tregs in inflammation, tissue remodeling, plaque contraction during regression. Methods...
Defective autophagy in macrophages leads to pathological processes that contribute atherosclerosis, including impaired cholesterol metabolism and defective efferocytosis. Autophagy promotes the degradation of cytoplasmic components lysosomes plays a key role catabolism stored lipids maintain cellular homeostasis. microRNA-33 (miR-33) is post-transcriptional regulator genes involved homeostasis, yet complete mechanisms by which miR-33 controls lipid are unknown. We investigated whether...
The progression of chronic liver diseases towards cirrhosis is accompanied by drastic tissue changes. This study combines elaborate transcriptomic and histological methods aiming at spatially resolving the hepatic immune microenvironment in NAFLD (including NASH, primary sclerosing cholangitis, biliary severe alcoholic hepatitis).Human samples were subjected to RNA-sequencing (n=225) imaging cytometry (n=99) across 3 independent patient cohorts. Liver from hepatitis cholangitis patients used...
Phenolic compounds combine antioxidant and hypocholesterolemic activities and, consequently, are expected to prevent or minimize cardiometabolic risk.To evaluate the effect of an aqueous extract (AQ) non-esterified phenolic fraction (NEPF) from rosemary on oxidative stress in diet-induced hypercholesterolemia, 48 male 4-week old Wistar rats were divided into 6 groups: 1 chow diet group (C) 5 hypercholesterolemic groups, with receiving water (HC), 2 AQ at concentrations 7 140 mg/kg body...
Stress granules (SGs) are dynamic cytoplasmic aggregates containing mRNA, RNA-binding proteins, and translation factors that form in response to cellular stress. SGs have been shown contribute the pathogenesis of several human diseases, but their role vascular diseases is unknown. This study shows accumulate smooth muscle cells (VSMCs) macrophages during atherosclerosis. Approach Results: Immunohistochemical analysis atherosclerotic plaques from LDLR-/- mice revealed an increase stress...
Macrophages accumulate prominently in the visceral adipose tissue (VAT) of obese humans and high fat diet (HFD) fed mice, this is linked to insulin resistance type II diabetes. While mechanisms regulating macrophage recruitment obesity have been delineated, signals directing persistence VAT are poorly understood. We previously showed that neuroimmune guidance cue netrin-1 expressed mice humans, where it promotes accumulation. To better understand source its effects on (ATM) fate function...
Rationale: MicroRNA-33 (miR-33 [post-transcriptionally in the rationale]) post-transcriptionally represses genes involved lipid metabolism and energy homeostasis. Targeted inhibition of miR-33 increases plasma HDL (high-density lipoprotein) cholesterol promotes atherosclerosis regression, part, by enhancing reverse transport dampening plaque inflammation. However, how reshapes immune microenvironment plaques remains poorly understood. Objective: To define alters dynamic balance...
Rationale: Therapeutic efforts to decrease atherosclerotic cardiovascular disease risk have focused largely on reducing atherogenic lipoproteins, yet lipid-lowering therapies alone are insufficient fully regress plaque burden. We postulate that arterial repair requires resolution of a maladaptive immune response and targeting factors hinder inflammation will facilitate regression. Objective: The guidance molecule Ntn1 (netrin-1) is secreted by macrophages in plaques, where it sustains...
MicroRNAs (miRNA) have emerged as important post-transcriptional regulators of metabolic pathways that contribute to cellular and systemic lipoprotein homeostasis. Here, we identify two conserved miRNAs, miR-224, miR-520d, which target gene networks regulating hepatic expression the low-density (LDL) receptor (LDLR) LDL clearance. In silico prediction miR-224 miR-520d showed they each repress multiple genes impacting LDLR, including chaperone molecules PCSK9 IDOL limit LDLR at cell surface...
Interesterified fats are being widely used by the food industry in an attempt to replace trans fatty acids. The effect of interesterified containing palmitic or stearic acids on lipid metabolism and inflammatory signaling pathways adipose hepatic tissues was evaluated. Male LDLr-KO mice were fed a high-fat diet polyunsaturated (PUFA), (PALM), (PALM INTER), (STEAR), (STEAR INTER) for 16 weeks. expression genes protein levels involved processes liver white tissue determined quantitative RT-PCR...
Bioactive compounds may be an alternative approach to prevent atherosclerosis. To evaluate this hypothesis, LDLr‐knockout mice were supplemented with omega‐3 fatty acids from Echium oil (10.24 mg/d of 1,14 SDA and 9.06 ALA) equivalent 0.7 EPA after conversion, combined or not phytosterols (0.76 mg/d), during the first 2 months life. Subsequently, dyslipidaemia was induced by a high‐fat diet for following months. oil, isolated phytosterols, improved lipid profile in plasma reducing...
Objective: Recent studies have identified key transcriptional regulators of brown adipose tissue (BAT) differentiation and function, but posttranscriptional control this network by microRNAs remains incompletely understood. MiR-33 critically regulates genes involved in metabolic pathways, including cholesterol efflux, reverse transport, fatty acid oxidation, autophagy. Given its role homeostasis, we investigated whether miR-33 participates the regulation BAT activity, white beiging, adaptive...
Atherosclerosis results from lipid-driven inflammation of the arterial wall that fails to resolve. Imbalances in macrophage accumulation and function, including diminished migratory capacity defective efferocytosis, fuel maladaptive plaque progression. The neuroimmune guidance cue netrin-1 has dichotomous roles partly due its multiple receptors; atherosclerosis, promotes survival retention via receptor Unc5b. To minimize pleiotropic effects targeting netrin-1, we tested therapeutic potential...
Experimental and clinical studies have demonstrated the effect of phytosterols (PS) on reducing plasma levels cholesterol LDL-c, but effects plant sterols beyond cholesterol-lowering are still questionable. Since inflammation endothelial dysfunction involved in pathogenesis atherosclerosis, this study aims to evaluate PS biomarkers atherosclerosis progression whether these independent alterations LDL-c levels. Thirty-eight moderately hypercholesterolemic volunteers (58 ± 12 years; ≥ 130...
ABSTRACT To delay lipid oxidation during meat processing, synthetic antioxidants have been used in the food industry, but consumers' concern over their toxicity increased interest research with natural antioxidants. The aim of this work was to analyze water activity ( A w ), thiobarbituric acid reactive substances (TBARS), moisture and trichloroacetic acid‐soluble nitrogen (TCASN) brined tilapia fillets treated or pretreated rosemary extract Rosmarinus officinalis ) stored for 240 days at −...
Because the plasma campesterol/cholesterol ratio does not differ between groups that absorb different amounts of cholesterol, authors investigated whether Phytosterols (PS) relate to body's cholesterol synthesis rate measured as non-cholesterol sterol precursors (lathosterol). The studied 38 non-obese volunteers (58±12 years; Low-Density Lipoprotein Cholesterol ‒ LDL-C ≥ 130 mg/dL) randomly assigned consume 400 mL/day soy milk (Control phase) or + PS (1.6 g/day) for four weeks in a...