A5052315484- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Inflammatory mediators and NSAID effects
- Influenza Virus Research Studies
- Asthma and respiratory diseases
- Microbial infections and disease research
- Ginseng Biological Effects and Applications
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Infectious Diseases and Tuberculosis
- Infectious Diseases and Mycology
- Biochemical and Molecular Research
- Cancer therapeutics and mechanisms
- Respiratory viral infections research
- Rheumatoid Arthritis Research and Therapies
- Vagus Nerve Stimulation Research
- Toxin Mechanisms and Immunotoxins
- Autoimmune and Inflammatory Disorders Research
- Pregnancy-related medical research
- Workplace Health and Well-being
- Occupational Health and Performance
- Medicinal Plant Pharmacodynamics Research
- Healthcare professionals’ stress and burnout
- Synthesis of β-Lactam Compounds
- Medieval and Early Modern Iberia
Centers for Disease Control and Prevention
2001-2025
Orlando Health
2025
Eli Lilly (United States)
2017
National Center for HIV/AIDS Viral Hepatitis STD and TB Prevention
2006-2010
University of South Florida
2002
National Center for Infectious Diseases
2000-2001
Purdue University West Lafayette
1956
ABSTRACT Ethionamide (ETH) is a structural analog of the antituberculosis drug isoniazid (INH). Both these drugs target InhA, an enzyme involved in mycolic acid biosynthesis. INH requires catalase-peroxidase (KatG) activation, and mutations katG are major resistance mechanism. Recently (EthA) capable activating ETH has been identified. We sequenced entire ethA gene 41 ETH-resistant Mycobacterium tuberculosis isolates. also two regions inhA all or part katG. The MICs were determined order to...
Fluoroquinolone antibiotics are among the most potent second-line drugs used for treatment of multidrug-resistant tuberculosis (MDR TB), and resistance to this class is one criterion defining extensively drug resistant (XDR TB). in Mycobacterium has been associated with modification quinolone determining region (QRDR) gyrA. Recent studies suggest that amino acid substitutions gyrB may also play a crucial role resistance, but functional genetic these mutations M. lacking. In study, we...
ABSTRACT We examined the correlation of mutations in pyrazinamidase (PZase) gene ( pncA ) with pyrazinamide (PZA) resistance phenotype 60 Mycobacterium tuberculosis isolates. PZase activity was determined by method Wayne (L. G. Wayne, Am. Rev. Respir. Dis. 109: 147–151, 1974), and entire nucleotide sequence, including 74 bp upstream start codon, determined. PZA susceptibility testing performed proportions on modified Middlebrook Cohn 7H10 medium. The MICs were ≥100 μg/ml for 37 isolates, 34...
Objective To determine whether valdecoxib, at chronic arthritis doses, has the characteristics of a cyclo-oxygenase 2 (COX-2) specific inhibitor, as measured by reduced incidence upper-gastrointestinal ulceration compared with conventional nonsteroidal anti-inflammatory drugs (NSAIDs). Methods This double-blind, multicentre, placebo-controlled, parallel-group study gastroduodenal ulcers associated valdecoxib 10 mg daily (q.d.) and 20 q.d. that ibuprofen 800 three times (t.i.d.) or diclofenac...
The current study describes the development of a unique real-time PCR assay for detection mutations conferring drug resistance in Mycobacterium tuberculosis. rifampicin determinant region (RRDR) rpoB and specific regions katG inhA promoter were targeted rifampin (RIF) isoniazid (INH) resistance, respectively. Additionally, this was multiplexed to discriminate tuberculosis complex (MTC) strains from nontuberculous Mycobacteria (NTM) by targeting IS6110 insertion element. High-resolution...
ABSTRACT Mycobacterium abscessus and chelonae are two closely related species that often not distinguished by clinical laboratories despite the fact they cause diseases requiring different treatment regimens. Multilocus enzyme electrophoresis, PCR-restriction fragment length polymorphism analysis of 65-kDa heat shock protein gene, biochemical tests, high-performance liquid chromatography mycolic acids were used to identify 75 isolates as either M. or originally submitted for drug...
ABSTRACT The TB-Biochip oligonucleotide microarray system is a rapid to detect mutations associated with rifampin (RIF) resistance in mycobacteria. After optimizing the 29 laboratory-generated rifampin-resistant mutants of Mycobacterium tuberculosis , we evaluated performance this test using 75 clinical isolates . With small sample set, displayed sensitivity 80% and specificity 100% relative conventional drug susceptibility testing results for RIF resistance. For these samples (∼50% tested...
Background: Evaluation of optimal dosing has generally been inadequate during TB drug development. Fluoroquinolones are central to treatment. We aimed determine the dose levofloxacin needed achieve maximal efficacy and acceptable safety tolerability as part a multidrug regimen. Methods: Opti-Q was an international, multi-center, randomized, placebo-controlled, phase II trial. Eligible participants with resistant isoniazid rifampicin but susceptible fluoroquinolones (MDR-TB) were randomized...
Respiratory viral infections pose a significant health problem that disproportionately affects the elderly. With aging worldwide population being less responsive to protective vaccines, there is an urgent need for strategies can protect elderly from community acquired infections. Background/Objectives: Allo-Priming novel immunomodulatory vaccine concept using allogeneic, living, activated Th1 cells are rejected by host, creating anti-alloantigen immunity, increasing titers. orchestrate...
Respiratory viral infections pose a significant health problem that disproportionately affects the elderly. With aging worldwide population being less responsive to protective vaccines, there is an urgent need for strategies can protect elderly from community-acquired infections. Background/Objectives: Allo-priming novel immunomodulatory vaccine concept using allogeneic, living, activated Th1 cells are rejected by host, creating anti-alloantigen immunity, increasing titers. orchestrate...
ABSTRACT Multidrug-resistant (MDR) isolates of Mycobacterium tuberculosis complex (MTBC) are defined by resistance to at least rifampin (RMP) and isoniazid (INH). Rapid accurate detection multidrug is essential for effective treatment interruption disease transmission (TB). Overdiagnosis MDR TB may result in with second-line drugs that more costly, less effective, poorly tolerated than first-line drugs. CDC offers rapid confirmation the molecular drug (MDDR) mutations associated RMP INH...
Ethambutol (EMB) is used as a part of drug regimens for treatment tuberculosis (TB). Susceptibility Mycobacterium complex (MTBC) isolates to EMB can be discerned by DNA sequencing detect mutations in the embB gene associated with resistance. US Public Health Laboratories (PHL) primarily use growth-based susceptibility test (DST) methods determine The Centers Disease Control and Prevention (CDC) provides service molecular detection resistance (MDDR) concurrent DST using agar proportion. PHL...
Mycobacterium tuberculosis complex isolates from cerebrospinal fluid of 67 meningitis patients were obtained six fever hospitals in Egypt. One M. bovis and 66 identified by PCR-restriction fragment length polymorphism (RFLP) analysis oxyR. Among the isolates, 53 unique strain types (with 3 to 16 copies IS6110) found RFLP analyses. Nine clusters (eight with two each one isolates) also found. Thirty-six spoligotypes, including at least 10 that have been previously reported other countries,...