A5046180274- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Plant Reproductive Biology
- Epigenetics and DNA Methylation
- Plant nutrient uptake and metabolism
- Nitrogen and Sulfur Effects on Brassica
- Plant Disease Resistance and Genetics
- Plant Genetic and Mutation Studies
- Ubiquitin and proteasome pathways
Peking University
2022-2025
Center for Life Sciences
2022-2025
In eukaryotes, three-dimensional (3D) chromatin architecture maintains genome stability and is important in regulating gene transcription. However, little known about the mechanisms by which diverse ATP-dependent remodeling complexes regulate 3D structure plants. We examined within ATPase subunit of SWI/SNF, ISWI, INO80, CHD wild-type (WT) mutant Arabidopsis thaliana plants combining high-throughput sequencing with situ Hi-C, enrichment histone marks, nucleosome density, expression. found...
Abstract Three-dimensional (3D) chromatin organization is highly dynamic during development and seems to play a crucial role in regulating gene expression. Self-interacting domains, commonly called topologically associating domains (TADs) or compartment (CDs), have been proposed as the basic structural units of organization. Surprisingly, although these found several plant species, they escaped detection Arabidopsis (Arabidopsis thaliana). Here, we show that genome partitioned into...
Abstract PWWP-DOMAIN INTERACTOR OF POLYCOMBS (PWO) family proteins play a vital role in regulating plant development. However, the molecular mechanisms of how PWOs regulate chromatin structure is elusive. Our data show that PWO1 binding sites are enriched with positive modifications but exclusive H3K27me3. Moreover, binds to H3K27me3-enriched compartment domain (H3K27me3-CD) boundary regions, and functions maintain strength. Meanwhile, we found Polycomb repressive complex 2 (PRC2) function...
The histone H3K4 trimethylation (H3K4me3) is widely distributed at numerous actively transcribed protein-coding genes throughout the genome. However, interplay between H3K4me3 and other chromatin modifications remains poorly understood in plants. In this study, we find that Arabidopsis thaliana H3K4me3-binding ALFIN-LIKE (AL) proteins are associated with H3K4me3-enriched whole-genome level. AL contain a C-terminal PHD finger, which has conserved role recognizing H3K4me3, PHD-associated (PAL)...
Promoters serve as key elements in the regulation of gene transcription. In mammals, loop interactions between promoters and enhancers increase complexity promoter-based regulatory networks. However, identification enhancer-promoter or promoter-related loops Arabidopsis remains incomplete.