A5103041884- Diabetes, Cardiovascular Risks, and Lipoproteins
- Intraocular Surgery and Lenses
- Retinal Development and Disorders
- Peroxisome Proliferator-Activated Receptors
- Retinal Diseases and Treatments
- Retinal and Optic Conditions
- Lipid metabolism and disorders
- RNA and protein synthesis mechanisms
- Ocular Infections and Treatments
- Diabetes and associated disorders
- Lipoproteins and Cardiovascular Health
- Photoreceptor and optogenetics research
- Retinal and Macular Surgery
- Pancreatic function and diabetes
- Cholesterol and Lipid Metabolism
- Retinopathy of Prematurity Studies
- Artificial Intelligence in Healthcare and Education
- Glioma Diagnosis and Treatment
- Brain Metastases and Treatment
- Surgical Simulation and Training
- Opioid Use Disorder Treatment
- Retinal Imaging and Analysis
- Child Abuse and Related Trauma
- Explainable Artificial Intelligence (XAI)
- CCD and CMOS Imaging Sensors
West Coast Retina
2024-2025
California Pacific Medical Center
2024-2025
University of California, San Francisco
2020-2023
Columbia University Irving Medical Center
2020
San Francisco Foundation
2020
Columbia University
2014-2015
Baylor College of Medicine
1976-2008
Methodist Hospital
1976
Abstract Human hepatic lipase is an important enzyme in high density lipoprotein (HDL) metabolism, being implicated the conversion of HDL2 to HDL3. Three human cDNA clones were identified two lambda gt11 libraries from liver. The cDNA-derived amino acid sequence predicts a protein 476 residues, preceded by 23-residue signal peptide. Four potential N-glycosylation sites are identified, which conserved rat lipase. On alignment with human, mouse, and bovine lipase, same also all three species....
We examined the structure-function relationship of human lipoprotein lipase (hLPL) in its ability to enhance binding and catabolism very low density lipoproteins (VLDL) COS cells. Untransfected cells did not bind or catabolize normal VLDL. Expression wild-type hLPL by transient transfection enhanced binding, uptake, degradation VLDL (a property LPL that we call bridge function). Heparin pretreatment a monoclonal antibody ID7 blocks LDL receptor-binding domain apoE both inhibited apoE2/E2...
While the past decade has seen great progress in mapping loci for common diseases, studying how these risk alleles lead to pathology remains a challenge. Age-related macular degeneration (AMD) affects 9 million older Americans, and is characterized by loss of retinal pigment epithelium (RPE). Although closely linked genome-wide association studies ARMS2/HTRA1 genes, located at chromosome 10q26 locus, are strongly associated with AMD, their downstream targets unknown. Low population...
Apolipoprotein (apo) B-48 mRNA is produced by in vivo RNA editing which involves a C----U conversion of the first base codon CAA for Gln-2153, changing it to UAA, an in-frame stop codon. We have reproduced reaction vitro using nuclear extracts. Efficient was demonstrated apoB segments as substrate or coupled transcription-editing minigenes template. ApoB were constructed ligating adenovirus major late promoter fragment apoB-100 DNA containing site and used reaction. defined sequence...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEffects of an atherogenic diet on apolipoprotein E biosynthesis in the ratYen-Chiu Lin-Lee, Yoshio Tanaka, Chin-Tarng Lin, and Lawrence ChanCite this: Biochemistry 1981, 20, 22, 6474–6480Publication Date (Print):October 1, 1981Publication History Published online1 May 2002Published inissue 1 October 1981https://pubs.acs.org/doi/10.1021/bi00525a028https://doi.org/10.1021/bi00525a028research-articleACS PublicationsRequest reuse permissionsArticle...
Abstract The biological features of adipose stromal (stem) cells (ASC), which serve as progenitors for differentiated white tissue (WAT), are still largely undefined. In an initiative to identify functional ASC surface receptors, we screened a combinatorial library peptide ligands binding patient-derived ASC. We demonstrate that both primary and cultured human mouse express conserved receptor targeted by peptides found mimic SPARC, matricellular protein is required normal WAT development. A...
Abstract The structure of human lipoprotein lipase was recently deduced from its cDNA sequence. It contains 8 serine residues (residues 45, 132, 143, 172, 193, 244, 251, and 363) that are absolutely conserved in both hepatic across all species studied. high homology between lipase, pancreatic suggests the catalytic functions these enzymes share a common mechanism one serines must play role as does 152 case (Winkler, F. K., D'Arcy, A., Hunziker, W. Nature 343, 771-774). We expressed wild-type...
Purpose: To study the feasibility and efficacy of a new remote wet lab for microsurgical education using corneal suturing task. Setting: Department Ophthalmology, University California San Francisco, California, USA. Design: Prospective randomized controlled study. Methods: Ten ophthalmology residents were stratified by postgraduate year to perform task consisting placing 4 cardinal sutures penetrating keratoplasty in porcine eyes with or without attending feedback. Subsequently, both groups...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCharacterization, cell-free synthesis and processing of apolipoprotein A-I rat high-density lipoproteinsMing-Huey Lin-Su, Yen-Chiu Lin-Lee, William A. Bradley, Lawrence ChanCite this: Biochemistry 1981, 20, 9, 2470–2475Publication Date (Print):April 1, 1981Publication History Published online1 May 2002Published inissue 1 April 1981https://pubs.acs.org/doi/10.1021/bi00512a017https://doi.org/10.1021/bi00512a017research-articleACS PublicationsRequest...
Journal Article Two polymorphisms for amino acid substitutions in the APOA4 gene Get access Eric Boerwinkle, Boerwinkle * *To whom correspondence should be addressed Search other works by this author on: Oxford Academic PubMed Google Scholar Sophia Visvikis, Visvikis 1Center Preventive MedicineNancy, France Lawrence Chan 2Departments of Cell Biology and Medicine, Baylor College MedicineHouston, TX 77030, USA Nucleic Acids Research, Volume 18, Issue 16, 25 August 1990, Page 4966,...
Purpose: Myopic macular degeneration (MMD) can cause irreversible vision loss. Thinner choroid is associated with increased MMD severity. This cross-sectional study analyzed choriocapillaris (CC) alterations in MMD. Methods: Axial length (AL), best-corrected visual acuity (BCVA), fundus photography, and swept-source optical coherence tomography angiography (SS-OCTA) were assessed controls high myopes (spherical equivalent ≤ −6 diopters). patients grade 2 (macular diffuse chorioretinal...
We have produced transgenic mice expressing human lipoprotein lipase (LPL) driven by the mouse metallothionein I promoter.We found that integration of LPL gene construct was associated with a high perinatal mortality.Animals survived first 2 weeks life grew normally afterwards.Compared controls, animals had higher post-heparin plasma and tissue activities.Immunoreactive detected in their but not controls.Transgenic significantly lower very low density (VLDL) while on regular laboratory...
Abstract Retinitis pigmentosa (RP) is described as a bilateral disease with inter-eye symmetry that presents on short-wavelength fundus autofluorescence (SW-AF) imaging hyperautofluorescent (hyperAF) rings an ellipsoid shape and regular borders. Nevertheless, both asymmetry irregular ring morphologies are also observed. In this retrospective study of 168 RP patients, we characterize the degree frequency hyperAF according to mode inheritance disease-causing gene by using SW-AF spectral-domain...
To determine treatment patterns and outcomes of pneumatic retinopexy (PnR) for rhegmatogenous retinal detachments (RRD).
Purpose: Infectious keratitis is a vision-threatening condition requiring close follow-up and disciplined eye drop administration to achieve resolution. Although patients presenting county hospitals often have more severe presentations, there paucity of risk outcomes data in this setting. This study investigates factors predicting loss (LTFU), medication noncompliance, poor for infectious the hospital Methods: was retrospective case-control at Zuckerberg San Francisco General Hospital Trauma...
Purpose: Infectious keratitis is a serious cause of visual impairment, particularly in low-income communities. This study examines the associations between social risk factors and polymicrobial keratitis, multidrug resistance, pathogen spectrum, outcomes at county hospital. Methods: We performed retrospective Zuckerberg San Francisco General Hospital patients treated for infectious from 2010 to 2021. Multivariable regression was analyze relationships social, medical, psychiatric with growth,...
Apolipoprotein (apo) B-100 mRNA is edited in the small intestine (in all mammals examined) and liver mice rats only) to produce apoB-48 mRNA. ApoB editing involves a C-->U conversion of first base codon CAA for Gln-2153 apoB-100, changing it an in-frame stop (UAA). The encodes apoB-48, which colinear with N-terminal 48% apoB-100. can be reproduced vitro using cellular extracts from one species edit synthetic apoB sequences different species. Editing transcripts transfected genes also appears...
Journal Article A unique length polymorphism in the signal peptide region of apolipoprotein B gene Mexican-Americans Get access Eric Boerwinkle, Boerwinkle Search for other works by this author on: Oxford Academic PubMed Google Scholar Craig L Hanis, Hanis Lawrence Chan 1Departments Cell Biology and Medicine, Baylor College MedicineHouston, TX 77030, USA Nucleic Acids Research, Volume 18, Issue 23, 1 December 1990, Page 7193, https://doi.org/10.1093/nar/18.23.7193 Published: 01 January 1990
Frontier AI safety policies highlight automation of research and development (R&D) by agents as an important capability to anticipate. However, there exist few evaluations for R&D capabilities, none that are highly realistic have a direct comparison human performance. We introduce RE-Bench (Research Engineering Benchmark, v1), which consists 7 challenging, open-ended ML engineering environments data from 71 8-hour attempts 61 distinct experts. confirm our experts make progress in the given 8...
To report a challenging case of spontaneous hyphema in the setting prone positioning for COVID-19 pneumonia.A previously healthy patient was concomitantly diagnosed with acute myelogenous leukemia (AML) and infection. During his hospitalization he required intubation positioning. Following change from to supine positioning, noted have developed large unilateral hyphema.We present due hematologic malignancy pneumonia.