A5038963228- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- SARS-CoV-2 and COVID-19 Research
- Cancer, Hypoxia, and Metabolism
- 3D Printing in Biomedical Research
- T-cell and B-cell Immunology
- Cancer, Lipids, and Metabolism
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Chromatin Remodeling and Cancer
- Cancer Immunotherapy and Biomarkers
- Renal cell carcinoma treatment
- Extracellular vesicles in disease
- Cancer Risks and Factors
- Immunotherapy and Immune Responses
- COVID-19 Impact on Reproduction
- CAR-T cell therapy research
- Pregnancy and preeclampsia studies
- Urinary and Genital Oncology Studies
- Radiomics and Machine Learning in Medical Imaging
- Bladder and Urothelial Cancer Treatments
Pratt Institute
2020-2023
Duke University
2020-2023
SARS-CoV-2 infection triggers profound and variable immune responses in human hosts. Chromatin remodeling has been observed individuals severely ill or convalescing with COVID-19, but chromatin early disease prior to anti-spike protein IgG seroconversion not defined. We performed the Assay for Transposase-Accessible using sequencing (ATAC-seq) RNA-seq on peripheral blood mononuclear cells (PBMCs) from outpatients mild moderate symptom severity at different stages of clinical illness. Early...
Ovarian cancer (OC) is the most lethal gynecological malignancy, with aggressive metastatic disease responsible for majority of OC-related deaths. In particular, OC tumors preferentially metastasize to and proliferate rapidly in omentum. Here, we show that cells experience increased oxidative stress omental microenvironment. Metabolic reprogramming, including upregulation pentose phosphate pathway (PPP), a key cellular redox homeostasis mechanism, allows compensate this challenge. Inhibition...
SARS-CoV-2 infection triggers highly variable host responses and causes varying degrees of illness in humans. We sought to harness the peripheral blood mononuclear cell (PBMC) response over course provide insight into COVID-19 physiology. analyzed PBMCs from subjects with symptom severity at different stages clinical before after IgG seroconversion SARS-CoV-2. Prior seroconversion, PBMC transcriptomes did not distinguish severity. In contrast, changes chromatin accessibility were associated...
ABSTRACT Sexual dimorphisms in immune responses contribute to coronavirus disease 2019 (COVID-19) outcomes, yet the mechanisms governing this disparity remain incompletely understood. We carried out sex-balanced sampling of peripheral blood mononuclear cells from confirmed COVID-19 inpatients and outpatients, uninfected close contacts, healthy controls for 36-color flow cytometry single cell RNA-sequencing. Our results revealed a pronounced reduction circulating mucosal associated invariant...
3076 Background: Preclinical models that can recapitulate patients’ intra-tumoral heterogeneity and microenvironment are crucial for tumor biology research drug discovery. In particular, the ability to retain immune other stromal cells in is vital development of immuno-oncology assays. However, current patient-derived organoid (PDO) largely devoid components. Methods: We first developed an automated microfluidic membrane platform generate tens thousands micro-organospheres from resected or...
Abstract SARS-CoV-2 infection triggers profound and variable immune responses in human hosts. Chromatin remodeling has been observed individuals severely ill or convalescing with COVID-19, but chromatin early disease prior to anti-spike protein IgG seroconversion not defined. We performed the Assay for Transposase-Accessible using sequencing (ATAC-seq) RNA-seq on peripheral blood mononuclear cells (PBMCs) from outpatients mild moderate symptom severity at different stages of clinical...
Summary Ovarian cancer (OC) is the most lethal gynecological malignancy, with aggressive metastatic disease responsible for majority of ovarian related deaths. In particular, OC tumors preferentially metastasize to and proliferate rapidly in omentum. Here, we show cells experience increased oxidative stress omental microenvironment. Metabolic reprogramming, including upregulation pentose phosphate pathway (PPP), a key cellular redox homeostasis mechanism, allows compensate this challenge....
Ovarian cancer (OC) is the most lethal gynecological malignancy, with aggressive metastatic disease responsible for majority of ovarian related deaths. In particular, OC tumors preferentially metastasize to and proliferate rapidly in omentum. Here, we show cells experience increased oxidative stress omental microenvironment. Metabolic reprogramming, including upregulation pentose phosphate pathway (PPP), a key cellular redox homeostasis mechanism, allows compensate this challenge. Inhibition...