A5030681754- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- HIV Research and Treatment
- Cellular transport and secretion
- RNA regulation and disease
- Plant Stress Responses and Tolerance
- Endoplasmic Reticulum Stress and Disease
- Plant Molecular Biology Research
- Engineering and Material Science Research
- Multiple and Secondary Primary Cancers
- Vibrio bacteria research studies
- Inflammatory Bowel Disease
- Hepatitis B Virus Studies
- Virus-based gene therapy research
- Liver physiology and pathology
- High voltage insulation and dielectric phenomena
- Genetic Neurodegenerative Diseases
- Plant Pathogenic Bacteria Studies
- Receptor Mechanisms and Signaling
- RNA and protein synthesis mechanisms
- Endometrial and Cervical Cancer Treatments
- Pharmacological Receptor Mechanisms and Effects
- Construction Project Management and Performance
- Microscopic Colitis
Albert Einstein College of Medicine
2018-2021
People's History Museum
2020
Cornell University
2019
The University of Texas Southwestern Medical Center
2018
Southwestern Medical Center
2018
Yale University
2013-2016
Whitney Museum of American Art
2015
Biotechnology Institute
2013
University of Delaware
2013
Cedars-Sinai Medical Center
2006
Significance Torsins belong to the AAA+ (ATPases associated with a variety of cellular activities) ATPase superfamily, members which disassemble protein complexes or unfold proteins. Here, we provide evidence that activity is tightly regulated by two proteins reside in endoplasmic reticulum and perinuclear space. This regulatory mechanism provides framework for better understanding phenotypes animal models, allows us define molecular defect underlying TorsinA dystonia.
Epidemiologic studies suggest that obese women are more likely to die of ovarian cancer than those ideal body weight, but it is not known whether increased incidence, comorbidities common women, or altered tumor biology responsible for this difference. The current study attempted determine the influence excess weight on survival, disease progression, and clinicopathologic factors.The records patients undergoing surgery epithelial at Cedars Sinai Medical Center between January 1, 1996 June...
Significance Torsin activity critically depends on accessory cofactors that activate their ATPase by a poorly understood mechanism. This study establishes the mechanistic framework for activation, which relies complementation of fragmentary active site. Given these unusual properties and fact activation is dysregulated in congenital movement disorder primary dystonia, our results suggest pharmacological manipulation may present novel therapeutic opportunity.
MicroRNAs (miRNAs) are a class of small RNAs that typically function by guiding the cleavage target messenger RNAs. They have been shown to play major roles in variety plant processes, including development, and responses pathogens environmental stresses. To identify new miRNAs regulation Arabidopsis (Arabidopsis thaliana), 27 RNA libraries were constructed sequenced from various tissues, stresses, biogenesis mutants, resulting 95 million genome-matched sequences. The use rdr2 enrich miRNA...
Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that causes persistent arthritis in subset of human patients. We report the isolation and functional characterization monoclonal antibodies (mAbs) from two patients infected with CHIKV Dominican Republic. Single B cell sorting yielded panel 46 mAbs diverse germline lineages targeted epitopes within E1 or E2 glycoproteins. MAbs recognized either proteins exhibited neutralizing activity. Viral escape mutations localized binding for...
ABSTRACT TorsinA is a membrane-tethered AAA+ ATPase implicated in nuclear envelope dynamics as well the egress of herpes simplex virus 1 (HSV-1). The activity and related TorsinB strictly depends on LAP1 LULL1, type II transmembrane proteins that are integral parts Torsin/cofactor AAA ring, forming composite, membrane-spanning assembly. Here, we use CRISPR/Cas9-mediated genome engineering to create single- double knockout (KO) cell lines TorA TorB their activators, investigate effect HSV-1...
Abstract The alphavirus capsid protein (Cp) selectively packages genomic RNA (gRNA) into the viral nucleocapsid to produce infectious virus. Using photoactivatable ribonucleoside crosslinking and an innovative biotinylated Cp retrieval method, here we comprehensively define binding sites for Semliki Forest virus (SFV) on gRNA. While data in infected cells demonstrate proposed genome packaging signal (PS), mutagenesis experiments show that PS is not required production of SFV or Chikungunya...
Alphaviruses are enveloped positive-sense RNA viruses that can cause serious human illnesses such as polyarthritis and encephalitis. Despite their widespread distribution medical importance, there no licensed vaccines or antivirals to combat alphavirus infections. Berberine chloride (BBC) is a pan-alphavirus inhibitor was previously identified in replicon-based small-molecule screen. This work showed BBC inhibits replication but also suggested might have additional effects later the viral...
Epidemic RNA viruses seem to arise year after leading countless infections and devastating disease. SARS-CoV-2 is the most recent of these viruses, but there will undoubtedly be more come. While effective vaccines are being deployed, one approach that still missing antivirals can used at onset therefore prevent pandemics. Here, we screened FDA-approved compounds against SARS-CoV-2. We found atovaquone, a pyrimidine biosynthesis inhibitor, able reduce infection in human lung cells. In...
Alphaviruses are small enveloped viruses with positive-sense RNA genomes. During infection, the alphavirus capsid protein (Cp) selectively packages and assembles viral genomic to form nucleocapsid core, a process critical production of infectious virus. Prior studies Semliki Forest virus (SFV) showed that packaging assembly promoted by Cp binding multiple high affinity sites on RNA. Here, we developed an in vitro assay based fluorescently labeled oligos. We used this explore sequence...
One way plants respond to environmental stress is by modifying their gene expression through the use of microRNAs (miRNAs). miRNAs are noncoding small RNAs that regulate at posttranscriptional level base pairing with complementary messenger RNA (mRNA) molecules, causing either mRNA cleavage or translational inhibition. To elucidate roles in responses, wild type and miRNA enriched mutant were subjected various stresses. Small libraries from stress‐treated seedlings flowers constructed...
Background: Lusutrombopag is an oral, small molecule thrombopoietin receptor agonist approved in Japan and US for improvement of thrombocytopenia, EU severe thrombocytonia, associated with chronic liver disease patients undergoing planned invasive procedures. The degree thrombocytopenia may impact the treatment response to lusutrombopag need platelet transfusion. Aims: This analysis evaluates increase count maximum achieved according baseline count. Methods: L‐PLUS 1 2 were two similar phase...
ABSTRACT The alphavirus capsid protein (Cp) selectively packages genomic RNA (gRNA) into the viral nucleocapsid to produce infectious virus. Using photoactivatable ribonucleoside crosslinking and an innovative biotinylated Cp retrieval method, we comprehensively defined binding sites for Semliki Forest virus (SFV) on gRNA. While data in infected cells demonstrated proposed genome packaging signal (PS), mutagenesis experiments showed that PS was not required production of SFV or Chikungunya...
INTRODUCTION: Vedolizumab (VDZ) is a gut-selective, humanized, monoclonal, α4β7 integrin antibody approved for the treatment of moderately to severely active ulcerative colitis (UC) in adults. Mucosal healing associated with improved long-term outcomes and considered an important therapeutic goal UC (1). GEMINI 1 VARSITY controlled trials have shown high rates mucosal VDZ-treated patients (2,3). METHODS: We conducted systematic literature review on real-world effectiveness VDZ achieving...