A5088699892- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Long-Term Effects of COVID-19
- Microtubule and mitosis dynamics
- Cellular transport and secretion
- Animal Virus Infections Studies
- Immune Cell Function and Interaction
- NF-κB Signaling Pathways
- Cardiomyopathy and Myosin Studies
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Mosquito-borne diseases and control
- Cardiac electrophysiology and arrhythmias
- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Immunotherapy and Immune Responses
- CRISPR and Genetic Engineering
- Chemical synthesis and alkaloids
- Phosphodiesterase function and regulation
- Invertebrate Immune Response Mechanisms
- Viral Infections and Vectors
- Bacteriophages and microbial interactions
- Toxin Mechanisms and Immunotoxins
- Cytokine Signaling Pathways and Interactions
- Antifungal resistance and susceptibility
Albert Einstein College of Medicine
2019-2022
Montefiore Medical Center
2020-2022
Massachusetts College of Pharmacy and Health Sciences
2020
University of Arizona
2013-2014
San Diego State University
1999
The U.S. FDA has issued emergency use authorizations (EUAs) for multiple investigational monoclonal antibody (MAb) therapies the treatment of mild to moderate COVID-19.
Convalescent plasma with severe acute respiratory disease coronavirus 2 (SARS-CoV-2) antibodies (CCP) may hold promise as a treatment for 2019 (COVID-19). We compared the mortality and clinical outcome of patients COVID-19 who received 200 mL CCP spike protein IgG titer ≥ 1:2430 (median 1:47,385) within 72 hours admission propensity score-matched controls cared at medical center in Bronx, between April 13 May 4, 2020. Matching criteria were age, sex, body mass index, race, ethnicity,...
Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus that causes persistent arthritis in subset of human patients. We report the isolation and functional characterization monoclonal antibodies (mAbs) from two patients infected with CHIKV Dominican Republic. Single B cell sorting yielded panel 46 mAbs diverse germline lineages targeted epitopes within E1 or E2 glycoproteins. MAbs recognized either proteins exhibited neutralizing activity. Viral escape mutations localized binding for...
We previously reported that Rab1a is associated with asialoorosomucoid (ASOR)-containing early endocytic vesicles, where it required for their microtubule-based motility. In knockdown (KD) cell lines, ASOR failed to segregate from its receptor and, consequently, did not reach lysosomes degradation, indicating a defect in endosome sorting. Although Rab1 Golgi/endoplasmic reticulum trafficking, this process was unaffected, likely due retained expression of Rab1b these cells. The present study...
Hydroxychloroquine, chloroquine, and azithromycin have been used for treatment of COVID-19, but may cause QT prolongation. Minority populations are disproportionately impacted by COVID-19. This study evaluates the risk prolongation subsequent outcomes after administration these medications in largely underrepresented minority COVID-19 patients.
The clinical outcome of SARS-CoV-2 infection varies widely between individuals. Machine learning models can support decision making in healthcare by assessing fatality risk patients that do not yet show severe signs COVID-19. Most predictive rely on static demographic features and values obtained upon hospitalization. However, time-dependent biomarkers associated with COVID-19 severity, such as antibody titers, substantially contribute to the development more accurate models. Here we trained...
Summary There is an urgent need for vaccines and therapeutics to prevent treat COVID-19. Rapid SARS-CoV-2 countermeasure development contingent on the availability of robust, scalable, readily deployable surrogate viral assays screen antiviral humoral responses, define correlates immune protection, down-select candidate antivirals. Here, we describe a highly infectious recombinant vesicular stomatitis virus bearing spike glycoprotein S as its sole entry that closely resembles authentic agent...
The coronavirus disease 2019 (COVID-19) global pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) continues to place an immense burden on societies and health care systems. A key component of COVID-19 control efforts is serological testing determine the community prevalence SARS-CoV-2 exposure quantify individual immune responses prior infection or vaccination. Here, we describe a laboratory-developed antibody test that uses readily available research-grade reagents detect...
Abstract Convalescent plasma with severe acute respiratory disease coronavirus 2 (SARS-CoV-2) antibodies (CCP) may hold promise as treatment for Coronavirus Disease 2019 (COVID-19). We compared the mortality and clinical outcome of patients COVID-19 who received 200mL CCP a Spike protein IgG titer ≥1:2,430 (median 1:47,385) within 72 hours admission to propensity score-matched controls cared at medical center in Bronx, between April 13 May 4, 2020. Matching criteria were age, sex, body mass...
The COVID-19 global pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) continues to place an immense burden on societies and healthcare systems. A key component of control efforts is serologic testing determine the community prevalence SARS-CoV-2 exposure quantify individual immune responses prior infection or vaccination. Here, we describe a laboratory-developed antibody test that uses readily available research-grade reagents detect in patient blood samples...
Abstract The IL-2 growth hormone is the major factor of activated T lymphocytes during a developing immune response. required not only for cell cycle progression but also to protect Ag-activated cells from programmed death. In several types, activation NF-κB and/or activating protein-1 (AP-1) has been demonstrated be extremely important in blocking apoptosis. To determine whether either or both these transcription factors are involved survival response IL-2, primary human responsive were...
Alzheimer’s Diseases (AD) is a neurodegenerative disorder characterized by progressive neuronal loss leading to cognitive decline. Although there yet be cure nor way reverse the damage, are current treatments amend some of symptoms associated with AD. Acetylcholinesterase inhibitors (AChEi) primary agents choice and have had profound implications throughout past decades. AChEi such as donepezil, rivastigmine, galantamine mediates increases cholinergic activities in central nervous system...
Abstract Most known SARS-CoV-2 neutralizing antibodies (nAbs), including those approved by the FDA for emergency use, inhibit viral infection targeting receptor-binding domain (RBD) of spike (S) protein. Variants concern (VOC) carrying mutations in RBD or other regions S reduce effectiveness many nAbs and vaccines evading neutralization. Therefore, therapies that are less susceptible to resistance urgently needed. Here, we characterized memory B-cell repertoire COVID-19 convalescent donors...
Abstract The coexistence of bicuspid aortic valve disease and coronary artery is well‐established, but the identification cardiac amyloidosis in this population has surged with advancing imaging techniques, introducing complexities patient management. This case report emphasizes pivotal role multimodality accurately diagnosing three concurrent pathologies.
Rab1a regulates endocytic vesicle processing by recruiting the microtubule‐based motor Kifc1. The mechanism which this occurs is unknown and therefore we examined whether Kifc1 interact with each other either directly or through a protein complex. Human embryonic kidney‐derived HEK293 cell lines expressing super folder green fluorescent (SFGFP) Rab1a‐SFGFP fusion were prepared. To test interacts Rab1a, plasmid encoding Kifc1‐flag was transfected into cells. Cell lysates subjected to...
Heat shock proteins (Hsps) are highly conserved molecules that constitutively expressed and upregulated in response to physiological stress conditions. These immunogenic chaperones can have essential functions fungi, particularly dimorphic pathogens. Histoplasma capsulatum Paracoccidioides species fungi the causative agents of histoplasmosis paracoccidioidomycosis, respectively, which systemic mycoses with significant rates morbidity mortality. Current treatment consists long-term antifungal...