A5017040893- Celiac Disease Research and Management
- Wheat and Barley Genetics and Pathology
- Microscopic Colitis
- Transgenic Plants and Applications
- Plant Genetic and Mutation Studies
- Crop Yield and Soil Fertility
- Photosynthetic Processes and Mechanisms
- Microbial Metabolites in Food Biotechnology
- Agriculture, Plant Science, Crop Management
- Antioxidant Activity and Oxidative Stress
- Clostridium difficile and Clostridium perfringens research
- Plant Micronutrient Interactions and Effects
- Genetically Modified Organisms Research
- Plant tissue culture and regeneration
- Food composition and properties
Instituto de Agricultura Sostenible
2015-2019
Università degli Studi della Tuscia
2019
Consejo Superior de Investigaciones Científicas
2015-2016
Summary Gluten proteins are responsible for the viscoelastic properties of wheat flour but also triggering pathologies in susceptible individuals, which coeliac disease ( CD ) and noncoeliac gluten sensitivity may affect up to 8% population. The only effective treatment affected persons is a strict gluten‐free diet. Here, we report effectiveness seven plasmid combinations, encompassing RNA i fragments from α‐, γ‐, ω‐gliadins, LMW glutenin subunits, silencing expression different prolamin...
Gluten proteins are major determinants of the bread making quality wheat but also important gluten-related disorders. The gluten protein accumulation during grain filling is strongly influenced by nitrogen fertilization. We have characterized in low-gliadin lines as treatments two experiments. These transgenic lines, D783, D793, C655, D577 and E82 were obtained using different RNAi silencing fragments endosperm-specific promoters to drive (D-hordein γ-gliadin). In Experiment 1 we used three...
The study evaluated the symptoms, acceptance, and digestibility of bread made from transgenic low-gliadin wheat, in comparison with gluten free bread, Non-coeliac sensitivity (NCGS) patients, considering clinical/sensory parameters gut microbiota composition. This was performed two phases seven days each, comprising a basal phase an E82 bread. Gastrointestinal clinical symptoms were using Symptom Rating Scale (GSRS) questionnaire, stool samples collected for immunogenic peptides (GIP)...
Macro- and micronutrients, essential for the maintenance of human metabolism, are assimilated daily through diet. Wheat other major cereals a good source nutrients, such as carbohydrates proteins, but cannot supply sufficient amount including provitamin A. As vitamin A deficiency (VAD) leads to several serious diseases throughout world, biofortification staple crop, wheat, represents an effective way preserve health in developing countries. In present work, key enzyme involved branch...
Abstract Some studies have suggested that the immunogenicity of oats depends on cultivar. RP-HPLC has been proposed as a useful technique to select varieties with reduced immunogenicity. The aim this study was identify both avenin protein patterns associated low gluten content and available variability for development new non-toxic oat cultivars. peaks alcohol-soluble avenins collection landraces cultivars characterized based elution times. immunotoxicity patients celiac disease (CD) tested...
Scope The aim of this work was to assess the ability Near Infrared Spectroscopy (NIRS) distinguish wheat lines with low gliadin content, obtained by RNA interference (RNAi), from non-transgenic lines. discriminant analysis performed using both whole grain and flour. transgenic sample set included 409 samples for sorting 414 flour experiments, while consisted 126 156 flour, respectively. Methods Results Samples were scanned a Foss-NIR Systems 6500 System II instrument. Discrimination models...
Macro and micronutrients, essential for the maintenance of human metabolism, are daily assimilated through diet. Wheat other major cereals a good source nutrients, such as carbohydrates proteins, but cannot supply enough amounts micronutrients which includes provitamin A. As vitamin A deficiency (VAD) lead to several serious diseases spread worldwide, biofortification staple crop, wheat, represents an effective way preserve health in developing countries. In present work, key enzyme involved...