A5066115254- Telomeres, Telomerase, and Senescence
- Cellular Mechanics and Interactions
- DNA Repair Mechanisms
- Protist diversity and phylogeny
- Advanced biosensing and bioanalysis techniques
- Genetics, Aging, and Longevity in Model Organisms
- Microtubule and mitosis dynamics
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Invertebrate Immune Response Mechanisms
- Aquaculture Nutrition and Growth
- Physiological and biochemical adaptations
- RNA modifications and cancer
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Marine Biology and Environmental Chemistry
- Cancer Cells and Metastasis
- ATP Synthase and ATPases Research
- Genomics and Rare Diseases
- 3D Printing in Biomedical Research
- Genetic and rare skin diseases.
- Mitochondrial Function and Pathology
- RNA Research and Splicing
Centre for Biomedical Network Research on Rare Diseases
2012-2025
Universidad Autónoma de Madrid
2014-2025
Instituto de Investigaciones Biomédicas Sols-Morreale
2012-2025
Centro de Investigación Biomédica en Red
2012-2025
Plum Island Animal Disease Center
2025
United States Department of Agriculture
2025
Agricultural Research Service
2025
Instituto de Investigación de Enfermedades Raras
2012-2024
Instituto de Salud Carlos III
2021-2024
Hospital Universitario La Paz
2011-2022
The social amoeba Dictyostelium discoideum executes a multicellular development program upon starvation. This morphogenetic process requires the differential regulation of large number genes and is coordinated by extracellular signals. MADS-box transcription factor SrfA required for several stages development, including slug migration spore terminal differentiation.
Abstract Lung cancer is the top cause of cancer‐related deceases. One reasons development resistance to chemotherapy treatment. In particular, stem cells ( CSC s), can escape treatment and regenerate bulk tumor. this article, we describe a comparison between resistant cisplatin s, both derived from non‐small‐cell lung cell lines H460 A549. Cisplatin‐resistant were obtained after single with drug. s isolated by culture in defined media, under nonadherent conditions. The clonogenic, could be...
: Bifunctional DNA glycosylases employ an active site lysine or the N-terminus to form a Schiff base with abasic (AP site) excision repair (BER) intermediate. Cleaving this reversible structure is rate-determining step in initiation of 8-oxoguanine (8-oxoG) for glycosylase 1 (OGG1). The OGG1 AP lyase activity can be increased using small molecule binders, called organocatalytic switches, cleave backbone similar manner as bifunctional glycosylase. In search novel switches we here identify...
We have detected a significant increase in the levels of pp60c-src kinase activity associated with differentiation myeloid cell lines HL-60 and U-937. The induction becomes apparent approximately 14 hr after addition phorbol 12-myristate 13-acetate increases 20-fold by 72 hr. enhanced can be accounted for elevated c-src protein differentiated cells. When nonleukemic bone marrow cells were examined, progenitor exhibited low level activity. As these are allowed to differentiate culture,...
Age and comorbidity are the main determinants of COVID-19 outcome. Shorter leukocyte telomere length (TL), a hallmark biological aging, has been associated with worse outcomes. We sought to determine TL in patients severe requiring hospitalization analyze whether clinical outcomes post-COVID-19 manifestations shorter TL.We analyzed 251 PCR-confirmed COVID-19, hospitalized first months pandemics. determined PBL at admission by quantitative-PCR (qPCR) analysis patients. A healthy cohort from...
Clinical trials have shown that lentiviral-mediated gene therapy can ameliorate bone marrow failure (BMF) in nonconditioned Fanconi anemia (FA) patients resulting from the proliferative advantage of corrected FA hematopoietic stem and progenitor cells (HSPC). However, it is not yet known if revert affected molecular pathways diseased HSPC. Single-cell RNA sequencing was performed chimeric populations uncorrected HSPC co-existing BM therapy-treated patients. Our study demonstrates reverts...
Telomeres are nucleoprotein structures present at the terminal region of chromosomes. Mutations in genes coding for proteins involved telomere maintenance causative a number disorders known as telomeropathies. The genetic origin these diseases is heterogeneous and has not been determined significant proportion patients. This article describes characterization cohort Telomere length was by Southern blot quantitative PCR. Nucleotide variants were analyzed either high-resolution melting...
Abstract Idiopathic pulmonary fibrosis (IPF) is a progressive, late-onset disease marked by lung scarring and irreversible loss of function. Genetic factors significantly contribute to both familial sporadic cases, yet there are scarce evidence-based studies highlighting the benefits integrating genetics into management IPF patients. In this study, we performed whole-exome sequencing telomere length (TL) measurements on patients their relatives. We then identified rare deleterious variants...
African swine fever (ASF) is a lethal disease of domestic pigs that currently challenging production in large areas Eurasia and the Caribbean. The causative agent, ASF virus (ASFV), large, double-stranded, structurally complex virus. ASFV genome encodes for more than 160 proteins; however, functions most them are still process being characterized. Recently, gene I196L has been reported as critically involved pigs. We report here recombinant derived from Georgia 2010 isolate (ASFV-G) lacking...
Background/Objectives: Control of classical swine fever virus (CSFV) in endemic countries relies on vaccination using live attenuated vaccines (LAVs). Most these LAVs do not allow for the differentiation vaccinated animals from infected (DIVA) based their serological response. FlagT4G vaccine is a novel candidate that confers robust protective immunity early after and shows DIVA capabilities. Methods: This report presents characterization terms stability its genomic phenotypes assessed by...
The predominant X-linked form of Dyskeratosis congenita results from mutations in DKC1, which encodes dyskerin, a protein required for ribosomal RNA modification that is also component the telomerase complex. We have previously found expression an internal fragment dyskerin (GSE24.2) rescues activity dyskeratosis (X-DC) patient cells. Here we increased basal and induced DNA damage response occurred X-DC cells comparison with normal localized telomeres heterochromatin formation senescence....
DUSP6/MKP3 is a dual-specific phosphatase that regulates extracellular regulated kinase ERK1/2 and ERK5 activity, with an increasingly recognized role as tumor suppressor. In silico studies from Gene expression Omnibus (GEO) Cancer Genome atlas (TCGA) databases reveal poor prognosis in those Non-small cell lung cancer (NSCLC) patients low levels of DUSP6. agreement these data, here we show DUSP6 plays major the regulation migration, motility growth. We have found upregulation several genes...
Inherited bone marrow failure syndromes (IBMFSs) are a group of congenital rare diseases characterized by failure, anomalies, high genetic heterogeneity, and predisposition to cancer. Appropriate treatment cancer surveillance ideally depend on the identification mutated gene. A next-generation sequencing (NGS) panel genes could be 1 initial screening test carried out in comprehensive study IBMFSs, allowing molecular detection affected patients. We designed 2 NGS panels IBMFS genes: version...
A second isoform of the Artemia franciscana sarcoplasmic or endoplasmic reticulum Ca-ATPase has been identified through isolation cDNA clones.This differs from previously one only at C-terminal end protein.The last 6 amino acids former change to 30 hydrophobic in newly that have potentiality being an additional transmembrane domain.The two A. isoforms are highly homologous coded by mammalian and bird SERCAB gene, except extensions share their character but no significant acid homology.The...
Abstract Translation in vitro of mRNA and immunoprecipitation with specific rabbit antisera showed that the unglycosylated precursor polypeptides mouse Mac-1 lymphocyte function associated antigen (LFA-1) alpha subunits are 130,000 Mr 140,000 Mr, respectively. Furthermore, polysomes purified by using anti-Mac-1 IgG yielded a similar major product translation = 130,000. The LFA-1 subunit products immunologically noncross-reactive, showing differences between these related proteins not due to...
Dyskeratosis congenita is an inherited disease caused by mutations in genes coding for telomeric components. It was previously reported that expression of a dyskerin-derived peptide, GSE24.2, increases telomerase activity, regulates gene and decreases DNA damage oxidative stress dyskeratosis patient cells. The biological activity short peptides derived from GSE24.2 tested one them, GSE4, probed to be active, further characterized this article. Expression eleven amino acids long peptide...
Four cDNA clones coding for different Artemia actin isoforms have been isolated. Three of the contain complete sequences while fourth one lacks 145 bases, 49 amino terminal acids protein. The acid predicted four identified are highly homologous to insect actins as well vertebrate cytoplasmic actins. code mRNAs 5.2, 1.9, 1.6 and 1.8 kb, respectively, whose expression is regulated during development. present in cryptobiotic embryos other not. steady-state levels all increase development reach...
A homolog of the Serum Response Factor (SRF) has been isolated from Dictyostelium discoideum and its function studied by analyzing consequences gene disruption. The MADS-box region SRF (DdSRF) is highly conserved with those human, Drosophila yeast homologs. srfA a developmentally regulated expressed in prespore spore cells. This plays an essential role sporulation as disruption leads to abnormal morphology loss viability. mutant spores were round cellulose deposition seemed be partially...