A5028055258- Chromosomal and Genetic Variations
- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Genetic Mapping and Diversity in Plants and Animals
- Plant Taxonomy and Phylogenetics
- Plant Pathogens and Resistance
- Genetics and Plant Breeding
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Lipid metabolism and biosynthesis
- Plant Genetic and Mutation Studies
- Genetic diversity and population structure
- Plant Stress Responses and Tolerance
- Bioenergy crop production and management
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Genetic and Environmental Crop Studies
- Plant Pathogens and Fungal Diseases
- Plant Reproductive Biology
- Plant and Fungal Species Descriptions
- Turfgrass Adaptation and Management
- Botanical Research and Chemistry
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
Agricultural Research Service
2015-2025
Chinese Academy of Agricultural Sciences
1999-2025
Citrus Research Institute
2025
Southwest University
2025
Utah State University
2010-2022
United States Department of Agriculture
2011-2022
University of Chinese Academy of Sciences
2009-2020
Shandong Agricultural University
2020
Institute of Genetics and Developmental Biology
2009-2011
Chinese Academy of Sciences
2007-2011
Cloned human DNA fragments containing globin genes are transcribed in vitro to form discrete RNA species. One transcription unit is located approximately 1500 base pairs upstream from the G-gamma-globin gene. This transcript partially homologous a polymerase III template 1000 delta-globin gene and short distance downstream beta-globin
The lipid content of microalgae is regarded as an important indicator for biodiesel. Many attempts have been made to increase the through biochemical and genetic engineering. Significant accumulation in has achieved using engineering, such nitrogen starvation, but cell growth was severely limited. However, enrichment by engineering anticipated. In this study, GmDof4 from soybean (Glycine max), a transcription factor affecting Arabidopsis, transferred into Chlorella ellipsoidea. We then...
The genome composition of intermediate wheatgrass (IWG; Thinopyrum intermedium (Host) Barkworth and D.R. Dewey; 2n = 6x 42) is complex remains to be a subject ongoing investigation. This study employed fluorescence in situ hybridization (FISH) analyze the karyotype Th. its related species. With St2-80 probe derived from Pseudoroegneria strigosa pDb12H Dasypyrum breviaristatum, FISH analysis classified chromosomes as JvsJvsJrJrStSt. was established using pSc119.2-1, (GAA)10, AFA-3, AFA-4,...
Karyotypes were analyzed on 24 diploid taxa (mostly perennials) belonging to eight Triticeae genera, which are defined by genome content (basic set of seven chromosomes): (i) Agropyron (P genome), (ii) Thinopyrum (J (iii) Secale (R (iv) Hordeum (i (v) Pseudoroegneria (S (vi) Psathyrostachys (N (vii) Australopyrum (W and (viii) Critesion (H genome). In addition traditional karyotype preparations, the metaphase root-tip chromosomes an interactive microcomputer program that printed idiogram in...
Genomic in situ hybridization (GISH) and Southern of genome-specific RAPD markers were used to demonstrate that the E genome (including Ee Eb from Thinopyrum elongatum bessarabicum, respectively) St (from Pseudoroegneria species) two basic genomes ponticum. GISH also revealed centromeric region may be critical area discriminates Th. Of seven partial amphiploids isolated backcrossed progenies Triticum aestivum x ponticum hybrids, (lines 693 7631) have eight pairs chromosomes (or) genomes....
To elucidate the genome constitutions of tetraploid (2n = 4x 28) species Elytrigia caespitosa, Lophopyrum nodosum, and Pseudoroegneria geniculata ssp. scythica hexaploid 6x 42) Thinopyrum intermedium, meiotic pairing was studied in these as well 10 hybrids. Karyotype analysis with aceto-orcein stained root-tip cells performed for four hybrids T. bessarabicum E. P. scythica, intermedium. by Giemsa C-banding carried out three two triploid involving bessarabicum. The behaved strict...
Abstract Understanding the classification and biosystematics of species in Triticeae Dumort., an economically important tribe grass family (Poaceae), is not easy task, particularly for some perennial species. Does genomic analysis facilitate understanding evolutionary relationships these species? We reviewed literature published after 1984 to address questions concerning: (1) genome among monogenomic diploid species; (2) progenitors unknown Y Elymus polyploids, X Thinopyrum intermedium , Xm...
To test the presence of a J genome in type species Leymus, L. arenarius, its total genomic DNA and that tetraploids mollis, salinus ssp. salmonis, ambiguus, chinensis, secalinus, alaicus karataviensis, innovatus were probed with 277-bp insert pLeUCD2, which can hybridize J, S, P but not N, R, V, Q, I, T, ABD genomes. The probe hybridized PalI- or TaqI-digested DNAs from Thinopyrum elongatum (JeJe diploid) T. x Psathyrostachys juncea (JeN hybrid), those arenarius (NNNNXXXX octoploid) all...
Thinopyrum bessarabicum (2n = 14; JJ) was successfully crossed with T. elongatum EE) but the reciprocal cross failed. Five of 19 F 1 plants headed in a greenhouse without being vernalized. Spikes hybrids were intermediate to those parents for number florets per spike, glume length, and first rachis internode similar spike length spikelets respectively. Karyotypes mitotic chromosomes parental species revealed that three seven J E genomes arm ratio. Meiosis substantiated conclusion from...
ABSTRACT Stem rust (caused by Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. & E. Henn.), a devastating disease of wheat ( Triticum aestivum L.), was effectively controlled worldwide for the past 50 yr deployment stem resistance Sr ) genes in cultivars. However, new race, TTKSK (known as Ug99 or TTKS) that emerged eastern Africa, is cause concern because it has broad virulence to currently deployed genes. To identify potentially sources effective against Ug99, we evaluated and...
Defensins are small cationic peptides that could be used as the potential substitute for antibiotics. However, there is no efficient method producing defensins. In this study, we developed a new strategy to produce defensin in nitrate reductase (NR)-deficient C. ellipsoidea (nrm-4). We constructed plant expression vector carrying mutated NP-1 gene (mNP-1), mature α-defensin from rabbit with an additional initiator codon 5′-terminus, which selection markers were NptII and NR genes....
Intermediate wheatgrass (Thinopyrum intermedium (Host) Barkworth & D.R. Dewey), a segmental autoallohexaploid (2n = 6x 42), is not only an important forage crop but also valuable gene reservoir for wheat (Triticum aestivum L.) improvement. Throughout the scientific literature, there continues to be disagreement as origin of different genomes in intermediate wheatgrass. Genotypic data obtained from newly developed EST-SSR primers derived putative progenitor diploid species Pseudoroegneria...
Oil in the form of triacylglycerols (TAGs) is quantitatively most important storage energy for eukaryotic cells. Diacylglycerol acyltransferase (DGAT) considered rate-limiting enzyme TAG accumulation. Chlorella, a unicellular green alga, has attracted much attention as potential feedstock renewable production. However, function DGAT1 Chlorella not been reported.A full-length cDNA encoding putative diacylglycerol 1 (DGAT1, EC 2.3.1.20) was obtained from ellipsoidea. The 2,142 bp open reading...
Salt tolerance at germination and seedling growth stages was investigated. GWAS revealed nine genomic regions with pleiotropic effects on salt tolerance. tolerant genotypes were identified for future breeding program. With 20% of the irrigated land worldwide affected by it, salinity is a serious threat to plant development crop production. While wheat most stable food source worldwide, it has been classified as moderately salinity. In several plants; such barley, maize rice, shown that seed...
Genome analysis based on chromosome pairing in diploid hybrids of perennial Triticeae species was examined along with those interpretations data triploid and tetraploid (or) species. The mean arm frequency, c, known genome combinations used as a measure homology. trivalent frequency the averaged sum trivalents quadrivalents were indices triploids tetraploids, respectively, to assess validity c values. relative affinity, x value, respective also value combination. Positive correlation...
Diploid intergeneric hybrids were synthesized among diploid species of the genera Agropyron Gaertn. (P genome), Pseudoroegneria (Nevski) A. Löve (S Thinopyrum (J-E Psathyro-stachys Nevski (N Hordeum L. (H and Secale (R genome). Chromosome pairing patterns in these permit estimation genomic similarity various genomes. Measurements genome based on chromosome are generally agreement with those other measurements. It is consistent that Jb Je (= E) most closely-related genomes, genetic ranging...