A5100778358- Plant Genetic and Mutation Studies
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Research on Leishmaniasis Studies
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Plant-Microbe Interactions and Immunity
- Acute Ischemic Stroke Management
- RNA and protein synthesis mechanisms
- Plant Reproductive Biology
- Plant Pathogens and Fungal Diseases
- Mitochondrial Function and Pathology
- Mycorrhizal Fungi and Plant Interactions
- Plant biochemistry and biosynthesis
- Lipid metabolism and biosynthesis
- Transgenic Plants and Applications
- Fungal Biology and Applications
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Muscle Physiology and Disorders
- Plant Virus Research Studies
- Circular RNAs in diseases
- Light effects on plants
- Molecular Biology Techniques and Applications
Fujian Medical University
2023-2025
Fujian Provincial Hospital
2011-2025
Huzhou Central Hospital
2025
Huzhou University
2025
Zhejiang University
2025
Shanxi University of Finance and Economics
2025
University of Chinese Academy of Sciences
2022-2024
Zhongshan Hospital
2021-2024
National Clinical Research Center for Digestive Diseases
2024
State Grid Corporation of China (China)
2024
The complete sequences of chloroplast genomes provide wealthy information regarding the evolutionary history species. With advance next-generation sequencing technology, number completely sequenced is expected to increase exponentially, powerful computational tools annotating genome are in urgent need. We have developed a web server CPGAVAS. accepts sequence as input. First, it predicts protein-coding and rRNA genes based on identification mapping most similar, full-length protein, cDNA by...
Abstract The Brassica genus encompasses three diploid and allopolyploid genomes, but a clear understanding of the evolution agriculturally important traits via polyploidy is lacking. We assembled an juncea genome by shotgun single-molecule reads integrated to genomic genetic maps. discovered that A subgenomes B. napus each had independent origins. Results suggested were monophyletic origin evolved into vegetable-use oil-use subvarieties. Homoeolog expression dominance occurs between B . , in...
Ferroptosis, an iron-dependent lipid peroxidation-induced form of regulated cell death, shows great promise as a cancer therapy strategy. Despite the critical role mitochondria in ferroptosis regulation, underlying mechanisms remain elusive. This study reveals that mitochondrial protein METTL17 governs function colorectal (CRC) cells through epigenetic modulation. Bioinformatic analysis establishes expression positively correlates with resistance and is up-regulated CRC. Depletion sensitizes...
The lipid content of microalgae is regarded as an important indicator for biodiesel. Many attempts have been made to increase the through biochemical and genetic engineering. Significant accumulation in has achieved using engineering, such nitrogen starvation, but cell growth was severely limited. However, enrichment by engineering anticipated. In this study, GmDof4 from soybean (Glycine max), a transcription factor affecting Arabidopsis, transferred into Chlorella ellipsoidea. We then...
The clinical potential of miRNA-based liquid biopsy has been largely limited by the heterogeneous sources in plasma and tedious assay processes. Here, we develop a precise robust one-pot called dual-surface-protein-guided orthogonal recognition tumor-derived exosomes situ profiling microRNAs (SORTER) to detect exosomal miRNAs enhance diagnostic accuracy prostate cancer (PCa). SORTER uses two allosteric aptamers against marker CD63 tumor EpCAM create an labeling barcode achieve selective...
A novel, high-efficiency inverter using MOSFETs for all active switches is presented photovoltaic, non-isolated, AC module applications. The proposed H6-type configuration features high efficiency over a wide load range, low ground leakage current, no need split capacitors, and output AC-current distortion. detailed power stage operating principles, PWM scheme, novel bootstrap supply the are described. Experimental results of 300 W hardware prototype show that not only MOSFET body diode...
Existing photovoltaic (PV) panels have widely varying input voltages based on the panel configuration and size. In this paper, a novel topology with single active switch - combining boost, flyback, charge-pump circuits is proposed in order to achieve wide range, high voltage gain, efficiency, low cost simultaneously. It meets input-voltage power-level needs of majority modern PV while still being suitable for connection highvoltage dc bus. The circuit has been designed, simulated,...
Ganoderma lucidum is one of the well-known medicinal basidiomycetes worldwide. The mitochondrion, referred to as second genome, an organelle found in most eukaryotic cells and participates critical cellular functions. Elucidating structure function this genome important understand completely genetic contents G. lucidum. In study, we assembled mitochondrial analyzed differential expressions its encoded genes across three developmental stages. a typical circular DNA molecule 60,630 bp with GC...
Abstract Recurrent acute respiratory tract infections (ARTIs) affect a large population, yet the specific decisive factors are largely unknown. Here we study population of 4407 children diagnosed with ARTI, comparing virome and serum cytokine profiles associated multiple ARTIs single ARTI during six-year period. The relative abundance Propionibacterium phages is significantly elevated in compared to group. Serum levels TIMP-1 PDGF-BB markedly increased single-ARTI non-ARTI controls, making...
METTL8 has recently been identified as the methyltransferase catalyzing 3-methylcytidine biogenesis at position 32 (m3C32) of mitochondrial tRNAs. also potentially participates in mRNA methylation and R-loop biogenesis. How plays multiple roles distinct cell compartments catalyzes tRNA m3C formation remain unclear. Here, we discovered that alternative splicing generated several isoforms METTL8. One isoform (METTL8-Iso1) was targeted to mitochondria via an N-terminal pre-sequence, while...
Proofreading (editing) of mischarged tRNAs by cytoplasmic aminoacyl-tRNA synthetases (aaRSs), whose impairment causes neurodegeneration and cardiac diseases, is high significance for protein homeostasis. However, whether mitochondrial translation needs fidelity the editing aaRSs have been unclear. Here, we show that mammalian cells critically depended on threonyl-tRNA synthetase (mtThrRS, encoded Tars2 ), disruption which accumulated Ser-tRNA Thr generated a large abundance Thr-to-Ser...
Plastid ribosomal proteins (PRPs) are essential for ribosome biogenesis, plastid protein biosynthesis, chloroplast differentiation, and early development. This study identifies the first rice PRP mutant, asl1 (albino seedling lethality1), which exhibits an albino lethal phenotype at stage. was associated with altered chlorophyll (Chl) content Map-based cloning revealed that ASL1 encodes S20 (PRPS20), localizes to chloroplast. showed tissue-specific expression, as it highly expressed in...
Drought and soil salinity are major abiotic stresses. The mechanisms of stress tolerance have been studied extensively in model plants. Caragana korshinskii is characterized by high drought salt northwestern China; unique patterns gene expression allow it to tolerate the imposed dehydration semi-desert saline soil. There have, however, no reports on differences between C. plants underlying their regulation expression. Three sequencing libraries from salt-treated whole-seedling- control were...
Purple carrots can accumulate large quantities of anthocyanins in their roots and –in some genetic backgrounds- petioles, therefore they represent an excellent dietary source antioxidant phytonutrients. In a previous study, using linkage analysis carrot F2 mapping population segregating for root petiole anthocyanin pigmentation, we identified region chromosome 3 with co-localized QTL all pigments the root, whereas pigmentation segregated as single dominant gene mapped to one these "root...
Background The impact of serum matrix metalloproteinases‐9 (MMP‐9) on cognitive impairment after ischemic stroke is unclear. We aimed to investigate the association between MMP‐9 in short‐term acute phase and at 3 months. Methods Results Our study was based a subsample from CATIS (China Antihypertensive Trial Acute Ischemic Stroke); total 558 patients with levels 7 26 participating sites trial were included this analysis. Cognitive severity categorized as severe, mild, or none (Mini‐Mental...
Chloroplasts are essential for photosynthesis and play critical roles in plant development. In this study, we characterized the temperature-sensitive chlorophyll-deficient rice mutant tcd5, which develops albino leaves at low temperatures (20 °C) normal green high (32 °C). The development of chloroplasts etioplasts is impaired tcd5 plants 20 °C, period phenotype P4 stage leaf thylakoid membranes arrested mid-P4 °C. We performed positional cloning TCD5 then complementation knock-down...
The global outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) urgently requires an effective vaccine for prevention. In this study, 66 epitopes containing pentapeptides SARS-CoV-2 spike protein in the IEDB database were compared with amino acid sequence protein, and potentially immune-related peptides obtained. Based on single-nucleotide polymorphisms analysis 1218 isolates, 52 easily mutated sites identified used epitope screening. best candidate screened out through...
Methyltransferase-like 8 (METTL8) encodes a mitochondria-localized METTL8-Iso1 and nucleolus-distributed METTL8-Iso4 isoform, which differ only in their N-terminal extension (N-extension), by mRNA alternative splicing. generates 3-methylcytidine at position 32 (m3C32) of mitochondrial tRNAThr tRNASer(UCN). Whether is an active m3C32 methyltransferase the role N-extension tRNA formation remain unclear. Here, we revealed that was inactive generation due to lack N-extension, contains several...
This study aimed to obtain a high yield and purity of Sargassum pallidum polyphenol extracts (SPPE) its enzyme activity. Fresh seaweed was selected for optimization ultrasound-assisted extraction (UAE) conditions purification using macroporous resin Sephadex LH20 SPPE. The SPPE characterized UPLC-QTOF-MS/MS α-amylase, α-glucosidase, tyrosinase, AchE inhibitory activity were determined. maximum rate 7.56 mg GAE/g the reached 70.5% after LH-20 purification. A total 50 compounds identified by...
Abstract In women, breast cancer (BC) accounts for 7%–10% of all cases and is one the most common cancers. To identify a new method treating BC, role CD93 its underlying mechanism were explored. MDA‐MB‐231 cells used in this study transfected with si‐CD93, si‐MMRN2, oe‐CD93, si‐integrin β1, or oe‐SP2 lentivirus. After si‐NC they injected into nude mice by subcutaneous injection at dose 5 × 10 6 /mouse to construct BC animal model. The expression genes proteins cell migration, invasion...
Camelina (Camelina sativa L.), a hexaploid member of the Brassicaceae family, is an emerging oilseed crop being developed to meet increasing demand for plant oils as biofuel feedstocks. In other Brassicas, high oil content can be associated with yellow seed phenotype, which unknown camelina. We sought create camelina using CRISPR/Cas9 technology disrupt its Transparent Testa 8 (TT8) transcription factor genes and evaluate resulting phenotype. identified three TT8 genes, one in each...