A5102821084- Fibroblast Growth Factor Research
- Prostate Cancer Treatment and Research
- Epigenetics and DNA Methylation
- Hematopoietic Stem Cell Transplantation
- Inflammatory mediators and NSAID effects
- SARS-CoV-2 detection and testing
- RNA modifications and cancer
- Clinical Nutrition and Gastroenterology
- Cancer-related molecular mechanisms research
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- Ferroptosis and cancer prognosis
- Bacteriophages and microbial interactions
- Cancer, Hypoxia, and Metabolism
- Congenital heart defects research
- Pulmonary Hypertension Research and Treatments
- Drug Transport and Resistance Mechanisms
- Metabolism and Genetic Disorders
- Crystallization and Solubility Studies
- Cardiovascular Disease and Adiposity
- Hedgehog Signaling Pathway Studies
- Proteoglycans and glycosaminoglycans research
- Tissue Engineering and Regenerative Medicine
- Urological Disorders and Treatments
- Estrogen and related hormone effects
Xiamen University
2006-2025
Case Western Reserve University
2011-2022
The University of Texas Health Science Center at Houston
2007-2013
Texas A&M University
2007-2013
Baylor College of Medicine
2013
Texas A&M Health Science Center
2007-2011
Texas A&M University System
2004
Ablation of the kinases Mst1 and Mst2, orthologs Drosophila antiproliferative kinase Hippo, from mouse intestinal epithelium caused marked expansion an undifferentiated stem cell compartment loss secretory cells throughout small large intestine. Although median survival mice lacking Mst1/Mst2 is 13 wk, adenomas distal colon are common by this age. Diminished phosphorylation, enhanced abundance, nuclear localization transcriptional coactivator Yes-associated protein 1 (Yap1) evident in...
Agents that promote tissue regeneration could be beneficial in a variety of clinical settings, such as stimulating recovery the hematopoietic system after bone marrow transplantation. Prostaglandin PGE2, lipid signaling molecule supports expansion several types stem cells, is candidate therapeutic target for promoting vivo. Here, we show inhibition 15-hydroxyprostaglandin dehydrogenase (15-PGDH), prostaglandin-degrading enzyme, potentiates multiple organs mice. In chemical screen, identify...
Ferroptosis, an iron-dependent lipid peroxidation-induced form of regulated cell death, shows great promise as a cancer therapy strategy. Despite the critical role mitochondria in ferroptosis regulation, underlying mechanisms remain elusive. This study reveals that mitochondrial protein METTL17 governs function colorectal (CRC) cells through epigenetic modulation. Bioinformatic analysis establishes expression positively correlates with resistance and is up-regulated CRC. Depletion sensitizes...
The induction of ferroptosis is promising for cancer therapy. However, the mechanisms enabling cells to evade ferroptosis, particularly in low-cystine environments, remain elusive. Our study delves into intricate regulatory Activating transcription factor 3 (ATF3) on Cystathionine β-synthase (CBS) under cystine deprivation stress, conferring resistance colorectal (CRC) cells. Additionally, our findings establish a positively correlation between this signaling axis and CRC progression,...
The fibroblast growth factor (FGF) family consists of 22 members and regulates a broad spectrum biological activities by activating diverse isotypes FGF receptor tyrosine kinases (FGFRs). Among the FGFs, FGF7 FGF10 have been implicated in regulation prostate development tissue homeostasis signaling through FGFR2 isoform. Using conditional gene ablation with Cre-LoxP system mice, we demonstrate tissue-specific requirement for urogenital epithelial cells - precursors prostatic branching...
The fibroblast growth factor receptor 1 (FGFR1) is ectopically expressed in prostate carcinoma cells, but its functional contributions are undefined. In this study, we report the evaluation of a tissue-specific conditional deletion mutant generated an ARR2PBi(Pbsn)-Cre/TRAMP/fgfr1(loxP/loxP) transgenic mouse model cancer. Mice lacking fgfr1, cells developed smaller tumors that also included distinct cancer foci still expressing fgfr1 indicating focal escape from gene excision. Tumors with...
Chromatin regulatory landscape plays a critical role in many disease processes and embryo development. Epigenome sequencing technologies such as chromatin immunoprecipitation (ChIP-seq) assay for transposase-accessible with high-throughput (ATAC-seq) have enabled us to dissect the pan-genomic of cells tissues both time space dimensions by detecting specific state its corresponding transcription factors. Pioneered advancement immunoprecipitation-chip (ChIP-chip) technology, abundant epigenome...
The mammalian Cks family consists of 2 well-conserved small proteins, Cks1 and Cks2. has been shown to promote cell-cycle progression by triggering degradation p27(kip1). function Cks2 in somatic cells is not well understood although it required for the first metaphase/anaphase transition during meiosis. Emerging evidence shows that elevated expression often found a variety tumors, correlated with poor survival rate patients. Here we demonstrated were prostate tumors human animal models, as...
The cardiac outflow tract (OFT) is a developmentally complex structure derived from multiple lineages and often defective in human congenital anomalies. Although emerging evidence shows that fibroblast growth factor(FGF) essential for OFT development, the downstream pathways mediating FGF signaling progenitors remain poorly understood. Here, we report FRS2α (FRS2), an adaptor protein links receptor kinases to pathways, mediates crucial aspects of FGF-dependent development mouse. Ablation...
The fibroblast growth factor (FGF) regulates a broad spectrum of biological activities by activation transmembrane FGF receptor (FGFR) tyrosine kinases and their coupled intracellular signaling pathways. substrate 2alpha (FRS2alpha) is an FGFR interactive adaptor protein that links multiple pathways to the activated kinase. We previously showed FGFR2 in prostate epithelium important for branching morphogenesis acquisition androgen responsiveness. Here we show mice FRS2alpha uniformly...
Rapid identification and continuous surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants are critical for guiding the response to COVID-19 pandemic. Whole-genome sequencing (WGS) is a preferred tool this aim, but many laboratories suffer from lack resources support population-level sequencing. Here, we describe two PCR strategies targeting spike protein mutations identify Alpha, Delta, Omicron variants. Signature were selected using dedicated bioinformatic...
Currently, RPA-exo and RPA-nfo are the primary methods for RPA/RT-RPA probe assays, both of which have been widely applied to detection various targets. However, exhibits lower sensitivity compared with exo method, while lacks capability equipment-free visualization inherent RPA-nfo. Both approaches mentioned above limit broader application assays. To address those limitations, we developed a novel recombinase polymerase amplification (RPA) combined an E. coli RNase HII assay (RH-RPA). This...
Although the fibroblast growth factor (FGF) signaling axis plays important roles in cell survival, proliferation, and differentiation, molecular mechanism underlying how FGF elicits these diverse regulatory signals is not well understood.By using Frs2 null mouse embryonic (MEF) conjunction with inhibitors to multiple pathways, here we report that activates mTOR via receptor substrate 2 (FRS2)-mediated PI3K/Akt pathway, suppresses autophagy activity MEFs.In addition, pathway regulated...
The enzyme 15-prostaglandin dehydrogenase (15-PGDH) catalyzes the first step in degradation of prostaglandins including PGE2. It is a negative regulator tissue repair and regeneration multiple organs. Accordingly, inhibitors 15-PGDH are anticipated to elevate vivo levels PGE2 promote healing regeneration. small molecule SW033291 (1) inhibits with Ki = 0.1 nM vitro, doubles vivo, shows efficacy mouse models recovery from bone marrow transplantation, ulcerative colitis, partial hepatectomy....
Hematopoietic stem cell transplantation following myeloablative chemotherapy is a curative treatment for many hematopoietic malignancies. However, profound granulocytopenia during the interval between and marrow recovery exposes recipients to risks of fatal infection, significant source transplant-associated morbidity mortality. We have previously described discovery small molecule, SW033291, that potently inhibits prostaglandin degrading enzyme 15-PGDH, increases bone E2, accelerates murine...
Abstract The fibroblast growth factor (FGF) signaling family controls a broad spectrum of cellular processes in development and adult tissue homeostasis function, which is expressed almost all tissues at stages. FGF receptor substrate 2 alpha (FRS2α) an adaptor protein that recruits downstream substrates to the (FGFR) tyrosine kinase. Disruption Frs2 α gene mice abrogates activation mitogen‐activated kinase pathway by FGFR leads embryonic lethality day E7.5 post copulation. To circumvent...
Fibroblast growth factors (FGFs) are upstream activators of the mitogen-activated protein kinase pathway and mitogens in a wide variety cells. However, whether solely accounts for induction cell cycle or antiapoptotic activity FGF receptor (FGFR) tyrosine is not clear. Here we report that inducer Cks1, which triggers ubiquitination degradation p27(Kip1), associates with unphosphorylated form FGFR substrate 2 (FRS2), an adaptor phosphorylated by kinases recruits downstream signaling...
ABSTRACT Armored RNA has been increasingly used as both an external and internal positive control in nucleic acid-based assays for virus. In order to facilitate armored purification, a His 6 tag was introduced into the loop region of MS2 coat protein, which allows exposure multiple tags on surface during assembly. The His-tagged particles were purified homogeneity verified be free DNA contamination single run affinity chromatography. A fragment severe acute respiratory syndrome coronavirus...
Abstract Binding of the fibroblast growth factor (FGF) to FGF receptor (FGFR) tyrosine kinase leads autophosphorylation as well phosphorylation multiple downstream signaling molecules that are recruited either by direct binding or through adaptor proteins. The FGFR substrate 2 (FRS2) family consists two members, FRS2α and FRS2β, has been shown recruit molecules, including Grb2 Shp2, FGFR1. To better understand how FRS2 interacted with FGFR1, in vivo assays coexpressed FGFR1 recombinant...
Non-steroidal anti-inflammatory drugs prevent colorectal cancer by inhibiting cyclooxygenase (COX) enzymes that synthesize tumor-promoting prostaglandins. 15-hydroxyprostaglandin dehydrogenase (15-PGDH) is a tumor suppressor degrades Murine knockout of 15-PGDH increases susceptibility to azoxymethane-induced colon tumors. It also renders these mice resistant celecoxib, selective inhibitor inducible COX-2 during neoplasia. Similarly, humans with low colonic are adenoma prevention celecoxib....