A5039201771- melanin and skin pigmentation
- Hippo pathway signaling and YAP/TAZ
- Biochemical Analysis and Sensing Techniques
- Skin Protection and Aging
- Pharmacological Effects of Natural Compounds
- Genomics, phytochemicals, and oxidative stress
- Microbial Metabolism and Applications
- Phytochemicals and Antioxidant Activities
- Tea Polyphenols and Effects
- Wnt/β-catenin signaling in development and cancer
- Drug-Induced Hepatotoxicity and Protection
- Protein Hydrolysis and Bioactive Peptides
- Enzyme Production and Characterization
- Plant Pathogenic Bacteria Studies
- Wood Treatment and Properties
- Nicotinic Acetylcholine Receptors Study
- Antioxidant Activity and Oxidative Stress
- Insect Utilization and Effects
- Microbial Fuel Cells and Bioremediation
- Traditional and Medicinal Uses of Annonaceae
- NF-κB Signaling Pathways
- Autophagy in Disease and Therapy
- Cell death mechanisms and regulation
- Microbial Applications in Construction Materials
- Phytase and its Applications
Xiamen Medical College
2016-2025
Xiamen University of Technology
2016-2025
Zhongshan Hospital of Xiamen University
2022-2024
Xiamen University
2011-2015
Harvard University
2011
Massachusetts General Hospital
2011
Ablation of the kinases Mst1 and Mst2, orthologs Drosophila antiproliferative kinase Hippo, from mouse intestinal epithelium caused marked expansion an undifferentiated stem cell compartment loss secretory cells throughout small large intestine. Although median survival mice lacking Mst1/Mst2 is 13 wk, adenomas distal colon are common by this age. Diminished phosphorylation, enhanced abundance, nuclear localization transcriptional coactivator Yes-associated protein 1 (Yap1) evident in...
The role of the unfolded protein response (UPR) in tissue homeostasis remains largely unknown. Here we find that loss Mst1/2, mammalian Hippo orthologues, or their regulator WW45, leads to a remarkably enlarged endoplasmic reticulum (ER) size-associated UPR. Intriguingly, attenuation UPR by tauroursodeoxycholic acid (TUDCA) diminishes Mst1/2 mutant-driven liver overgrowth and tumorigenesis promoting nuclear exit degradation downstream effector Yap. Yap is required for activity ER expansion...
The transcriptional coactivator Yes-associated protein (YAP) plays an important role in organ-size control and tumorigenesis. However, how Yap gene expression is regulated remains unknown. This study shows that the Ets family member GABP binds to promoter activates YAP transcription. depletion of downregulates YAP, resulting a G1/S cell-cycle block increased cell death, both which are substantially rescued by reconstituting YAP. can be inactivated oxidative mechanisms, acetaminophen-induced...
Folium Microcos (FM), the leaves of paniculata L., shows various biological functions including antioxidant activity and α-glucosidase inhibitory effect. However, its therapeutic potential in acute liver injury is still unknown. This study investigated hepatoprotective effect underlying mechanisms polyphenol-enriched fraction (FMF) from Microcos. FMF exhibited strong free radical scavenging activities prevented HepG2/Hepa1-6 cells hydrogen peroxide- (H2O2-) induced ROS production apoptosis...
Abstract Background Tea ( Camellia sinensis L.) flowers will compete with tea leaves in nutrition and are abandoned as an undesirable by-product. In this study, the biological efficacy of was investigated. Further exploration its antifungal activity explained. Methods harvested from China were characterized term component, antioxidant ability, tyrosinase inhibition, ability. Chemical compounds analyzed by LC-MS. Disinfectant identified flowers, 2-ketobutyric acid exhibited against...
In traditional Chinese medicine, the root bark and leaves of Schima argentea are utilized to treat dysentery, parasitic infections, digestive disorders. this study, n-butanol extract S. (NBA) exhibited potent antioxidant properties, protecting HaCaT cells from UVB-induced damage, was abundant in phenolic flavonoid compounds. Using UPLC-QTOF-MS analysis, several antioxidants within NBA were identified. Among these, 3,4-dihydroxybenzoic acid, (+)-catechin, procyanidin B2 effectively reduced...
The fruit of Rosa laevigata Michx. (FR), a traditional Chinese herb utilized for the treatment variety diseases, has notably diverse pharmacological activities including hepatoprotective, anti-oxidant, and anti-inflammatory effects. Despite ongoing research on illustrating underlying mechanism FR, principal remained inadequately understood. In this study, we investigated in depth molecular actions ethanol extract FR (EFR) its potential targets using lipopolysaccharide (LPS)-stimulated RAW...
A polyphenol‐enriched fraction (PEF) from Acalypha wilkesiana , whose leaves have been traditionally utilized for the treatment of diverse medical ailments, was investigated anti‐inflammatory effect and molecular mechanisms by using lipopolysaccharide‐ (LPS‐) stimulated RAW 264.7 macrophages acetaminophen‐ (APAP‐) induced liver injury mouse model. Results showed that PEF significantly attenuated LPS‐induced nitric oxide (NO) prostaglandin E 2 (PGE ) production suppressed expression inducible...
Milkfish Chanos chanos is considered as the fourth largest aquaculture product. Collagen peptides from milkfish scales (MSCP) were used to evaluate characteristics including moisturizing, transepidermal water loss rate (TEWL), anti-skin aging estimation by matrix metalloproteinase (MMP) activity, anti-melanogenesis, and sun protection factor (SPF). MSCP revealed excellent capacity of moisture absorption was increased 20% after 4 h at 44% 65% relative humidities. This result also corresponds...
Background: Orcinol (3,5-dihydroxytoluene) has been reported to demonstrate inhibitory activity against mushroom tyrosinase. This study aims investigate the effect of orcinol on melanogenesis and its mechanism in B16F10 murine melanoma cells. Methods: The effects were determined spectrophotometrically by intracellular tyrosinase melanin content. expression melanogenesis-related gene signaling pathways was analyzed Western blot and/or qRT-PCR analyses. Results: reduced content attenuated...
Invasive fungal infections (IFIs) are fatally threatening to critical patients. The defensin as an antifungal protein can widely inhibit fungi. In this study, eight genes from different filamentous fungi were optimized by synonymous codon bias and heterologously expressed in Escherichia coli. Only the (AFP) Aspergillus giganteus was produced, whereas AFP its mutation of chitin-binding domain could not be expressed, thereby suggesting importance motif for folding. addition, recombinant (rAFP,...
A new piperidine alkaloid, microcosamine C (1), and one known compound, (2) were isolated from the leaves of Microcos paniculata. Structure elucidation was carried out using HR-ESI-MS, 1D 2D NMR spectroscopic methods by comparison with data reported in literature. The absolute configuration at C-3 hydroxy group 1 established a Mosher esterification procedure. Both isolates (1–2) evaluated for cytotoxicity against four selected tumour cell lines showed only weak activity RAW 264.7 line.
Glutamic acid is widely recognized as safe and has various applications in the medical food industries. This study demonstrated its significant inhibition of tyrosinase, acting a mixed-type inhibitor according to enzymatic kinetic analysis. Fluorescence spectroscopy analysis investigation tyrosinase activity under different pH confirmed that glutamic induced changes protein structure leading reduced through acidification binding effects. Additionally, was found inhibit L-DOPA auto-oxidation,...
Abstract Background Medical plants confer various benefits to human health and their bioconversion through microbial fermentation can increase efficacy, reduce toxicity, conserve resources produce new chemical components. In this study, the cholesterol-lowering monacolin K genes content produced by Monascus species were identified. The high-yield strain further fermented with medicinal plants. antioxidant anti-inflammatory activities, red pigment content, total phenolic metabolites in...
Plant-derived extracts are a promising source of new drugs. Schima superba is traditionally used in China for heat clearing, detoxification, and treatment furuncles. In this study, the anticandidal properties mechanism action S. (SSE) were explored using stem bark extract. SSE possessed high polyphenol saponin contents 256.6 ± 5.1 357.8 31.5 µg/mg, respectively. A clear inhibition zone was observed C. albicans growth through disc diffusion method 50% by 415.2 µg/mL. Transcriptomic analysis...
Ureolytic bacteria in 12 isolates, namely TCU1-TCU12, obtained from the soil with long-term application of urea fertilizer were screened and characterized. Urea degrading activity all isolates was ranging 2.4 to 22.9 mM. The ureolysis profiles corresponded fluctuations pH value. Urease inhibitors, including acetohydroxamic acid phenyl phosphorodiamidate, applied prove activity. Moreover, antifungal verified by TCU4 TCU5 strains. 16S rRNA gene sequence analysis revealed that among eight...
The exploration of defense peptides holds significant research potential for fungal inhibition. This study optimized the antifungal gene (PtAFP) from Pyrenophora tritici-repentis using synonymous codon bias. resulting protein PtAFP exhibited a characteristic antiparallel β-folded barrel structure, featuring three disulfide bonds, with initial 23 amino acids forming signal secretion sequence. Successful cloning into Escherichia coli yielded an approximately 10 kDa. Fluorescence analysis...