Abstract Background MicroRNAs (miRNAs) are a large class of tiny non-coding RNAs (~22-24 nt) that regulate diverse biological processes at the posttranscriptional level by controlling mRNA stability or translation. As molecular switch, canonical Wnt/β-catenin signaling pathway should be suppressed during adipogenesis; However, activation this leads to inhibition lipid depots formation. The aim our studies was identify miRNAs might involved in adipogenesis modulating WNT pathway. Here we...

Understanding the dynamics of muscle transcriptome during development and between breeds differing in growth is necessary to uncover complex mechanism underlying development. Herein, we present first transcriptome-wide longissimus dorsi research concerning Lantang (LT, obese) Landrace (LR, lean) pig 10 time-points from 35 days-post-coitus (dpc) 180 days-post-natum (dpn) using Solexa/Illumina's Genome Analyzer. The data demonstrated that myogenesis was almost completed before 77 dpc, but...

Porcine reproductive and respiratory syndrome (PRRS) has been one of the most economically important diseases affecting swine industry worldwide causes great economic losses each year. PRRS virus (PRRSV) replicates mainly in porcine alveolar macrophages (PAMs) dendritic cells (DCs) develops persistent infections, antibody-dependent enhancement (ADE), interstitial pneumonia immunosuppression. But molecular mechanisms PRRSV infection still are poorly understood. Here we report on first...

Porcine reproductive and respiratory syndrome virus (PRRSV) 1 2 differ in their recognition of CD163. Substitution porcine CD163 SRCR5 domain with a human CD163-like SRCR8 confers resistance to PRRSV but not 2. The deletion has been shown confer vivo both vitro. However, the anti-PRRSV activity modifying yet reported. Here, we describe highly efficient generation two pig breeds (Liang Guang Small Spotted Large White pigs) lacking short region SRCR5, including ligand-binding pocket. We...

Summary The innate immune response provides the initial defence mechanism against infection by other organisms. However, an excessive will cause damage to host tissues. In attempt identify microRNAs (miRNAs) that regulate in inflammation and homeostasis, we examined differential expression of miRNAs using microarray analysis spleens mice injected intraperitoneally with lipopolysaccharide (LPS) saline, respectively. Following challenge, observed 19 up‐regulated (1·5‐fold) LPS. Among these...

ABSTRACT The CRISPR/Cas9 system has emerged as an intriguing new technology for genome engineering. It utilizes the bacterial endonuclease Cas9 which, when delivered to eukaryotic cells in conjunction with a user‐specified small guide RNA (gRNA), cleaves chromosomal DNA at target site. Here we show that concurrent delivery of gRNAs designed two different sites human chromosome introduce double‐strand breaks and give rise targeted deletions intervening genomic segment. Predetermined segments...

Empty follicle syndrome (EFS) is a reproductive disorder in which no oocytes are retrieved during IVF. The existence of genuine EFS (GEFS) still controversial, and to date, only one missense mutation Luteinizing Hormone/Choriogonadotropin Receptor (LHCGR) has been reported be associated with this disease. Here, we describe GEFS patient non-consanguineous family from China. A 27-year-old woman presented 5-year history primary infertility LH resistance-like ovaries unequal sizes, but normal...

Porcine reproductive and respiratory syndrome viruses (PRRSVs) pose a serious threat to the swine industry in China, which has caused great difficulties for porcine (PRRS) immune prevention control, due its easily mutable recombinant nature. In this study, two novel PRRSV strains, were named GD-H1 GD-F1, isolated fully sequenced from pig farms Guangdong province, China. The phylogenetic analysis recombination revealed that GD-F1 generated by of NADC30-like NADC34-like strains different...

Abstract Histone lysine demethylase 4A (KDM4A) plays a crucial role in regulating cell proliferation, differentiation, development and tumorigenesis. However, little is known about the function of KDM4A muscle regeneration. Here, we found that conditional ablation skeletal caused impairment embryonic postnatal formation. The loss satellite cells led to defective regeneration blocked proliferation differentiation cells. Myogenic myotube formation KDM4A-deficient myoblasts were inhibited....

Bone morphogenetic protein 15 (BMP15) is strongly associated with animal reproduction and woman reproductive disease. As a multifunctional oocyte-specific secret factor, BMP15 controls female fertility follicular development in both species-specific dosage-sensitive manners. Previous studies found that played critical role ovulation rate mono-ovulatory mammalian species, especially sheep human, but study on knockout mouse model implied possibly has minimal impact of poly-ovulatory species....

Abstract High-mobility group box 2 (HMGB2) is an abundant, chromatin-associated protein that plays essential role in the regulation of transcription, cell proliferation, differentiation, and tumorigenesis. However, underlying mechanism HMGB2 adipogenesis remains poorly known. Here, we provide evidence deficiency preadipocytes impedes adipogenesis, while overexpression increases potential for adipogenic differentiation. Besides, depletion vivo caused decrease body weight, white adipose tissue...

Krüppel-like factor 4 (KLF4) is an evolutionarily conserved zinc finger-containing transcription that regulates diverse cellular processes such as cell proliferation, apoptosis, and differentiation. Our previous study showed KLF4 expression upregulated in skeletal muscle ontogeny during embryonic development pigs, suggesting its importance for function. We revealed here plays a critical role regeneration. Specific knockout of impaired formation further affecting physical activity also...

Abstract Background Chinese indigenous pigs are popular with consumers for their juiciness, flavour and meat quality, but they have lower production. Insulin-like growth factor 2 (IGF2) is a maternally imprinted that promotes skeletal muscle by regulating cell proliferation differentiation. A single nucleotide polymorphism (SNP) within intron 3 of porcine IGF2 disrupts binding site the repressor, zinc finger BED-type containing 6 (ZBED6), leading to up-regulation causing major effects on...

Abstract Background A splice mutation that causes skipping of exon 17 in the KIT gene is a major reason for dominant white phenotype pigs. Exon may be related to differences testis size and sperm quality among different pig breeds. Investigating effects on spermatogonia differentiation testicular development essential understanding genetic reduced fertility semen To better understand porcine spermatogenesis, we described an deletion mouse model ( Kit D17/+ ) constructed by simulating...

Background As an important factor affecting meat quality, intramuscular fat (IMF) content is a topic of worldwide concern. Emerging evidences indicate that microRNAs play roles in adipocyte differentiation. However, miRNAome has neither been studied during porcine preadipocyte differentiation, nor compared with subcutaneous preadipocytes. The objectives this study were to identify miRNAs involved adipogenesis primary preadipocytes, and determine whether adipocytes differ the expression...

Porcine reproductive and respiratory syndrome virus (PRRSV) is a continuous threat to the pig industry, causing high economic losses worldwide. Current vaccines have specific limitations in terms of their safety efficacy, so development novel antiviral drugs urgently required. The aim this study was evaluate inhibitory effects underlying molecular mechanisms antimicrobial peptide cecropin P1 (CP1) against PRRSV infection vitro. CP1 not only displayed extracellular virucidal activity PRRSV,...

Abstract Background Highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS) has caused large economic losses in swine industry recent years. However, current antiviral strategy could not effectively prevent control this disease. In research, five artificial microRNAs (amiRNAs) respectively targeted towards ORF5 (amirGP5-243, -370) ORF6 (amirM-82, -217,-263) were designed incorporated into a miRNA-based vector that mimics the backbone of murine miR-155 permits high...

Genome editors such as CRISPR/Cas9 and TALENs are at the forefront of research into methodologies for targeted modification mammalian genome. To date few comparative studies have been carried out to investigate difference genome editing characteristics between TALENs. While system has overtaken tool choice most groups working in this field, we hypothesized that there could be certain applications whereby application would specific benefits. Here compare TALEN tools introducing site-specific...

Background Porcine reproductive and respiratory syndrome virus (PRRSV) causes severe diseases affecting the swine industry worldwide. Currently, no vaccination regimen has proven sustained success in preventing PRRSV infection. Therefore, there is an urgent need for new antiviral strategies to control PRRSV. In this study, inhibitory effects molecular mechanism of antimicrobial peptide protegrin-1 (PG-1) isolated from porcine leukocytes against were evaluated vitro. Methods Marc-145 cells or...

Abstract Background Fertilization failure (FF) is a complex reproductive disorder characterized by the of pronuclei formation during fertilization. In addition to some cases caused iatrogenic problems and known genetic factors, there are still many unexplained aspects FF. Here, we aimed assess clinical characteristics two families experiencing primary infertility with Methods We have from China. All infertile couples presented similar phenotypes, that is, partial or total fertilization in...