A5068712418- Electrochemical Analysis and Applications
- Electrochemical sensors and biosensors
- Analytical Chemistry and Sensors
- Biofuel production and bioconversion
- Conducting polymers and applications
- Enzyme Production and Characterization
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Electrocatalysts for Energy Conversion
- Enzyme Catalysis and Immobilization
- Advanced biosensing and bioanalysis techniques
- Advanced Cellulose Research Studies
- Microbial Metabolic Engineering and Bioproduction
- Ionic liquids properties and applications
- Studies on Chitinases and Chitosanases
- Fuel Cells and Related Materials
- Metal Extraction and Bioleaching
- Electrowetting and Microfluidic Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Protein Hydrolysis and Bioactive Peptides
- Neonatal Health and Biochemistry
- thermodynamics and calorimetric analyses
- Biosensors and Analytical Detection
- Analytical chemistry methods development
- Advanced Chemical Sensor Technologies
Ōtani University
2008-2025
Shinshu University
2013-2024
Osaka University
2013
Tohoku University
2013
Nara University of Education
2013
University of Toyama
2013
Centre for Biomedical Engineering and Physics
2013
Osaka University of Pharmaceutical Sciences
2013
Fukui Prefectural University
2003-2010
University of Fukui
2010
A biochemical fuel cell was constructed using H2 as to produce H2O in the reaction with O2 at neutral pH and ambient temperature. The uses carbon felt an electrode material for both anode cathode anion exchange membrane a separator. anodic oxidation of accelerated by methyl viologen-mediated electrocatalysis bacterial cells Desulfoibrio ulgaris (Hildenborough) catalysts, cathodic reduction 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate)-mediated bilirubin oxidase catalyst....
Cellobiohydrolases are exoacting, processive enzymes that effectively hydrolyze crystalline cellulose. They have attracted considerable interest because of their role in both natural carbon cycling and industrial enzyme cocktails used for the deconstruction cellulosic biomass, but many mechanistic regulatory aspects heterogeneous catalysis remain poorly understood. Here, we address this by applying a deterministic model to real-time kinetic data with high temporal resolution. We two variants...
An electrode modified with immobilized whole cells of Desulfovibrio vulgaris (Hildenborough) produces an S-shaped voltammogram both cathodic- and anodic-catalytic-limiting currents in a methyl viologen-containing buffer saturated H2. Methyl viologen penetrates into the bacterial to serve as electron carrier reversible reaction hydrogenase functions electron-transfer mediator between electrode, thus producing catalytic for evolution consumption equation current that takes account explains...
The kinetics of cellulose hydrolysis have long been described by an initial fast rate, tapering rapidly off, leading to a process that takes days rather than hours complete. This behavior has mainly attributed the action cellobiohydrolases and often linked processive mechanism this exo-acting group enzymes. endo-glucanases (EGs) is far less investigated, partly due limited availability quantitative assay technologies. We used isothermal calorimetry monitor early time course insoluble three...
An amperometric enzyme biosensor for continuous detection of cellobiose has been implemented as an assay cellulases. We show that the initial kinetics cellobiohydrolase I, Cel7A from Trichoderma reesei, acting on different types cellulose substrates, semi-crystalline and amorphous, can be monitored directly in real-time by enzyme-modified electrode based dehydrogenase (CDH) Phanerochaete chrysosporium (Pc). PcCDH was cross-linked immobilized surface a carbon paste which contained mediator,...
Previously, we reported properties of a glycosylase belonging to GH-8 glycosyl hydrolase (GH) and having both chitosanase glucanase activities. This enzyme (D2), whose molecular mass (86 kDa) was the largest among group, has its catalytic domain at N-terminal region, discoidin (DD) C-terminal region. Although various chitosanases, chitinases glucanases have been known, DD is unique D2 enzyme. Glucanase chitinase, but not chitosanase, are known functional such as carbohydrate-binding module,...
The hydrolysis of raw starch catalyzed by glucoamylase has been studied with granules different sizes use an amperometric glucose sensor which the direct and continuous observation concentration can be achieved even in a thick suspension. initial rate enzymatic suspension increased increasing enzyme to approach saturation value was proportional amount substrate. Also, specific surface area experimental results explained well equations derived from three-step mechanism, consists adsorption...
The kinetics of glucoamylase-catalyzed hydrolysis starch granules from six different botanical sources (rice, wheat, maize, cassava, sweet potato, and potato) was studied by the use an electrochemical glucose sensor. A higher rate obtained as a smaller size used. adsorbed amount glucoamylase on granule surface per unit area did not vary very much with type examined, while catalytic constants enzyme (k(0)) were determined to be 23.3+/-4.4, 14.8+/-6.0, 6.2+/-1.8, 7.1+/-4.1, 4.6+/-3.0,...
Abstract The shape of a laminar, liquid jet emerging from round vertical tube into the atmosphere and total axial force exerted on filament at exit were investigated experimentally over wide range flow conditions. An integral invariant exact solution was used to check consistency between two kinds data. A new equation predict agreed moderately well with present experimental data as various previous results. key idea work is that knowledge will be very useful reasonably given condition by...