A5038913657- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Fatty Acid Research and Health
- Biochemical and Molecular Research
- Amino Acid Enzymes and Metabolism
- Lipid metabolism and biosynthesis
- Biofuel production and bioconversion
- Enzyme Structure and Function
- Diet, Metabolism, and Disease
- Enzyme-mediated dye degradation
- Polyamine Metabolism and Applications
- Metal-Catalyzed Oxygenation Mechanisms
- Probiotics and Fermented Foods
- Gut microbiota and health
- Pharmacogenetics and Drug Metabolism
- Diet and metabolism studies
- Microbial metabolism and enzyme function
- Algal biology and biofuel production
- Metabolism and Genetic Disorders
- Fungal and yeast genetics research
- Electrochemical sensors and biosensors
- Microbial bioremediation and biosurfactants
- Helicobacter pylori-related gastroenterology studies
- Enzyme Production and Characterization
- Microbial Metabolism and Applications
Kyoto University
2016-2025
Nippon Medical School
2022-2025
Kobe University
2023
National Institute of Biomedical Innovation, Health and Nutrition
2023
Shizuoka University
2023
University of Shizuoka
2023
Yamagata University
2023
The University of Tokyo
2023
Research Organization of Information and Systems
2023
Niigata University
2021
Fe(III)-respiring bacteria such as Shewanella species play an important role in the global cycle of iron, manganese, and trace metals are useful for many biotechnological applications, including microbial fuel cells bioremediation waters sediments contaminated with organics, metals, radionuclides. Several alternative electron transfer pathways have been postulated reduction insoluble extracellular subsurface minerals, Fe(III) oxides, by species. One potential mechanism involves secretion...
Significance Microorganisms in the gastrointestinal tract interact with their host many ways. Lipid metabolism by microbes generates multiple fatty acid species that can affect health. In representative gut bacterium Lactobacillus plantarum , we revealed a metabolism, saturation of polyunsaturated acid, hydroxy acids, oxo conjugated and partially saturated trans -fatty acids as intermediates. Furthermore, analysis mice suggests modifies composition host. Therefore, functional investigations...
Gut microbiota mediates the effects of diet, thereby modifying host metabolism and incidence metabolic disorders. Increased consumption omega-6 polyunsaturated fatty acid (PUFA) that is abundant in Western diet contributes to obesity related diseases. Although gut-microbiota-related pathways dietary PUFAs were recently elucidated, on physiological function remain unclear. Here, we demonstrate gut confers resistance high-fat (HFD)-induced by modulating metabolism. Supplementation...
Gut microbial metabolites of polyunsaturated fatty acids have attracted much attention because their various physiological properties. Dysfunction tight junction (TJ) in the intestine contributes to pathogenesis many disorders such as inflammatory bowel disease. We evaluated effects five novel gut on tumor necrosis factor (TNF)-α-induced barrier impairment Caco-2 cells and dextran sulfate sodium-induced colitis mice. 10-Hydroxy-cis-12-octadecenoic acid (HYA), a metabolite linoleic acid,...
Specific isomers of conjugated linoleic acid (CLA), a fatty with potentially beneficial physiological and anticarcinogenic effects, were efficiently produced from by washed cells Lactobacillus acidophilus AKU 1137 under microaerobic conditions, the metabolic pathway CLA production is explained for first time. The identified as cis-9, trans-11- or trans-9, cis-11-octadecadienoic trans-11-octadecadienoic acid. Preceding CLA, hydroxy acids 10-hydroxy-cis-12-octadecaenoic...
Abstract After screening 14 genera of lactic acid bacteria, Lactobacillus plantarum AKU 1009a was selected as a potential strain for CLA production from linoleic acid. Washed cells L. with high levels were obtained by cultivation in nutrient medium 0.06% (wt/vol) ( cis ‐9, ‐12‐octadecadienoic acid). Under the optimal reaction conditions free form substrate, washed produced 40 mg CLA/mL mixture (33% molar yield) 12% 108 h. The resulting two isomers, trans ‐11 (or ‐11)‐octadecadienoic (CLA1,...
The fructose/dioxygen biofuel cell, one of the direct electron transfer (DET)-type bioelectrochemical devices, utilizes fructose dehydrogenase (FDH) on anode and multi-copper oxidase such as bilirubin (BOD) cathode catalysts. power density in literature is limited by biocathode performance. We show that DET-type performance greatly improved, when or some related substances are adsorbed electrodes before BOD adsorption. Several data substrate modification induces appropriate orientation...
ABSTRACT Cytochrome P450 BM-3, a self-sufficient enzyme from Bacillus megaterium that catalyzes the subterminal hydroxylation of long-chain fatty acids, has been engineered into catalyst for oxidation polycyclic aromatic hydrocarbons. The activities triplet mutant (A74G/F87V/L188Q) towards naphthalene, fluorene, acenaphthene, acenaphthylene, and 9-methylanthracene were 160, 53, 109, 287, 22/min, respectively. Compared with wild type these hydrocarbons, those improved by up to 4 orders...
Haa1 is a transcriptional activator required for Saccharomyces cerevisiae adaptation to weak acids. Here we show that the constitutive HAA1-overexpressing strain acquired higher level of acetic acid tolerance. Under conditions stress, intracellular was significantly lower in cells than wild-type cells.
Gene manipulation tools for an arachidonic-producing filamentous fungus, Mortierella alpina 1S-4, have not been sufficiently developed. In this study, Agrobacterium tumefaciens-mediated transformation (ATMT) was investigated M. 1S-4 transformation, using the uracil-auxotrophic mutant (ura5(-) strain) of as a host strain and homologous ura5 gene selectable marker gene. Furthermore, omega3-desaturase, catalyzing conversion n-6 fatty acid to n-3 acid, overexpressed in by employing ATMT system....
Among dietary fatty acids with immunologic effects, ω-3 polyunsaturated acids, such as α-linolenic acid (ALA), have been considered factors that contribute to the differentiation of M2-type macrophages (M2 macrophages). In this study, we examined effect ALA and its gut lactic bacteria metabolites 13-hydroxy-9(Z),15(Z)-octadecadienoic (13-OH) 13-oxo-9(Z),15(Z)-octadecadienoic (13-oxo) on M2 from bone marrow-derived cells (BMDCs) investigated underlying mechanisms. BMDCs were stimulated ALA,...
Gut microbiota can regulate the host energy metabolism; however, underlying mechanisms that could involve gut microbiota-derived compounds remain to be understood. Therefore, in this study, we investigated effects of KetoA [10-oxo-12(Z)-octadecenoic acid]-a linoleic acid metabolite produced by lactic bacteria-on whole-body metabolism and found dietary intake enhance expenditure mice, thereby protecting mice from diet-induced obesity. By using Ca2+ imaging whole-cell patch-clamp methods, was...
Hydroxy FAs, one of the gut microbial metabolites PUFAs, have attracted much attention because their various bioactivities. The purpose this study was to identify lactic acid bacteria with ability convert linoleic (LA) hydroxy FAs. A screening process revealed that a bacterium, Lactobacillus acidophilus NTV001, converts LA mainly into 13-hydroxy-cis-9-octadecenoic and resulted in identification hydratase responsible, fatty 1 (FA-HY1). Recombinant FA-HY1 purified, its enzymatic...
Starch is considered a potential feedstock for biofuel production, particularly in light of the large-scale landfilling food waste and other starchy materials worldwide. Lipid accumulation by oleaginous yeast promising method biodiesel production from starch. However, most yeasts are grown on monosaccharides or oligosaccharides because they cannot directly utilize We therefore investigated starch-assimilation ability 1,200 yeasts. found that Cryptococcus terricola could be used fuel through...
The present study investigated the antiallergic and anti-inflammatory effects of 10-hydroxy-cis-12-octadecenoic acid (HYA), a novel gut microbial metabolite linoleic acid, in NC/Nga mice, model atopic dermatitis (AD). Feeding HYA decreased plasma immunoglobulin E level skin infiltration mast cells with concomitant decrease score. feeding TNF-α increased claudin-1, tight junction protein, levels mouse skin. Cytokine expression intestinal Peyer's patches suggested that improved Th1/Th2 balance...
Abstract Faecal lipopolysaccharides (LPS) have attracted attention as potent elements to explain a correlation between the gut microbiota and cardiovascular disease (CVD) progression. However, underlying mechanism of how specific bacteria contribute faecal LPS levels remains unclear. We retrospectively analysed data 92 patients found that abundance genus Bacteroides was significantly negatively correlated with levels. The controls showed higher than in CVD. endotoxin units LPS, determined by...