A5012884513- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- Lipid metabolism and biosynthesis
- Sirtuins and Resveratrol in Medicine
- Antioxidant Activity and Oxidative Stress
- Phytoestrogen effects and research
- Cardiovascular Disease and Adiposity
- GABA and Rice Research
- Diet and metabolism studies
- Metabolomics and Mass Spectrometry Studies
- Epigenetics and DNA Methylation
- Biochemical Analysis and Sensing Techniques
- Advanced MRI Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Genomics, phytochemicals, and oxidative stress
- Soybean genetics and cultivation
- Cancer, Lipids, and Metabolism
- Pancreatic function and diabetes
- Biochemical effects in animals
- Cardiac Imaging and Diagnostics
- Diabetes Treatment and Management
- Mitochondrial Function and Pathology
- Fibroblast Growth Factor Research
Kyoto University
2015-2024
Hiroshima University
2023
Saitama University
2020
Nagaoka University of Technology
2019
Nagaoka University
2019
Among dietary fatty acids with immunologic effects, ω-3 polyunsaturated acids, such as α-linolenic acid (ALA), have been considered factors that contribute to the differentiation of M2-type macrophages (M2 macrophages). In this study, we examined effect ALA and its gut lactic bacteria metabolites 13-hydroxy-9(Z),15(Z)-octadecadienoic (13-OH) 13-oxo-9(Z),15(Z)-octadecadienoic (13-oxo) on M2 from bone marrow-derived cells (BMDCs) investigated underlying mechanisms. BMDCs were stimulated ALA,...
Gut microbiota can regulate the host energy metabolism; however, underlying mechanisms that could involve gut microbiota-derived compounds remain to be understood. Therefore, in this study, we investigated effects of KetoA [10-oxo-12(Z)-octadecenoic acid]-a linoleic acid metabolite produced by lactic bacteria-on whole-body metabolism and found dietary intake enhance expenditure mice, thereby protecting mice from diet-induced obesity. By using Ca2+ imaging whole-cell patch-clamp methods, was...
Abstract Scope: Tomato is one of the most common crops worldwide and contains many beneficial compounds that improve abnormalities lipid metabolism. However, molecular mechanism underlying effect tomato on metabolism unclear. It has been commonly accepted peroxisome proliferator‐activated receptor α (PPARα) important targets for ameliorating Therefore, we focused activation PPARα attempted to detect active activating in tomato. Methods results: To identify such compounds, screened fractions...
Dyslipidemia is a major risk factor for development of several obesity-related diseases. The peroxisome proliferator-activated receptor α (PPARα) ligand-activated transcription that regulates energy metabolism. Previously, we reported 9-oxo-10,12-octadecadienoic acid (9-oxo-ODA) presented in fresh tomato fruits and acts as PPARα agonist. In addition to 9-oxo-ODA, developed 13-oxo-9,11-octadecadienoic (13-oxo-ODA), which an isomer present only juice. this study, explored the possibility...
Obesity causes excess fat accumulation in white adipose tissues (WAT) and also other insulin-responsive organs such as the skeletal muscle, increasing risk for insulin resistance, which can lead to obesity-related metabolic disorders. Peroxisome proliferator-activated receptor-α (PPARα) is a master regulator of fatty acid oxidation whose activator known improve hyperlipidemia. However, molecular mechanisms underlying PPARα activator-mediated reduction adiposity improvement disorders are...
The water extract of soybean seeds (Glycine max (L.) Merr.) is nearly tasteless, but "kokumi" taste sensation was confirmed upon addition a basic umami solution containing glutamic acid, inosine monophosphate, and sodium chloride. To identify the key contributors to in seeds, sensory-guided fractionation, sensory analyses, LC-MS/MS analyses were utilized. γ-glutamyl-tyrosine γ-glutamyl-phenylalanine identified as "kokumi taste"; specifically, these γ-glutamyl peptides imparted at low...
The mevalonate pathway is essential for the synthesis of isoprenoids and cholesterol. Adipose tissue known as a major site cholesterol storage; however, role local its synthesized remains unclear. In this study, adipose-specific pathway-disrupted (aKO) mice were generated through knockout 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase (HMGCR). aKO showed serious lipodystrophy accompanied with glucose lipid metabolic disorders hepatomegaly. These variations in dramatically reversed after...
PPARα is well known as a master regulator of lipid metabolism. activation enhances fatty acid oxidation and decreases the levels circulating cellular lipids in obese diabetic patients. Although target genes are widely known, little about alteration plasma liver metabolites during activation. Here, we report that metabolome analysis-implicated upregulation many lysoGP species bezafibrate (PPARα agonist) treatment. In particular, 1-palmitoyl lysophosphatidylcholine [LPC(16:0)] increased by...
Endoplasmic reticulum (ER) homeostasis is critical in maintaining metabolic regulation. Once it disrupted due to accumulated unfolded proteins, ER restored via activation of the protein response (UPR); hence, UPR affects diverse physiological processes. However, how stress influences adipocyte functions not well known. In this study, we investigated effect thermogenic capacity mice beige adipocytes. Here, show that expression uncoupling 1 (Ucp1) involved thermoregulation severely suppressed...
Abstract We report X-ray spectroscopic results for four giant solar flares that occurred on 2005 September 7 (X17.0), 8 (X5.4), 9 (X6.2), and 2006 December 5 (X9.0), obtained from Earth albedo data with the Imaging Spectrometer (XIS) board Suzaku . The good energy resolution of XIS (FWHM ∼ 100 eV) enables us to separate a number line-like features detect underlying continuum emission. These include Si He α , Ly S Ar Ca originating as well fluorescent K β Earth’s atmosphere. Absolute...
Obesity-induced inflammation plays a pivotal role in the pathogenesis of insulin resistance and type 2 diabetes. Xanthoangelol (XA) 4-hydroxyderrcin (4-HD), phytochemicals extracted from Angelica keiskei, have been reported to possess various biological properties. Whether XA 4-HD alleviate obesity-induced inflammation-induced adipocyte dysfunction was investigated.For vitro study, co-culture system composed macrophages adipocytes stimulated with conditioned medium derived fully...
Adipocyte differentiation plays a pivotal role in maintaining the production of small-size adipocytes with insulin sensitivity, and impaired adipogenesis is implicated resistance. 4-Hydroxyderricin (4-HD), phytochemical component Angelica keiskei, possesses diverse biological properties such as anti-inflammatory, antidiabetic, antitumor. In present study, we investigated effects 4-HD on adipocyte differentiation. promoted lipid accumulation 3T3-L1 cells, upregulated both peroxisome...
Scope Peroxisome proliferator‐activated receptor alpha (PPAR‐α) is a ligand‐activated transcription factor that regulates lipid and carbohydrate metabolism. We investigate the effects of naturally occurring PPAR‐α agonists, phytol, its metabolite phytanic acid, on obesity‐induced metabolic disorders using mouse model. Methods results A luciferase reporter assay shows acid potently activates among PPAR subtypes. In high‐fat‐diet‐induced, severely obese mice, phytol‐enriched diet increases...
Dietary soy reportedly protects from diabetic nephropathy (DN), but its active components and mechanism of action remain unknown.In this study, KKAy mice are fed three types diet: isoflavones with protein (Soy-IP) diet, reduced (RisoP), oral administration aglycones (IsoAgc). Albuminuria glycosuria decreased only in the soy-IP group. The risoP group show expansion mesangial matrix renal fibrosis, IsoAgc anti-fibrotic anti-inflammatory effects; however, these pathological changes repressed...
Obesity-induced chronic inflammation is a key factor in type 2 diabetes. A vicious cycle involving pro-inflammatory mediators between adipocytes and macrophages common cause of the adipose tissue. Tomato one most popular vegetables associated with reduced risk However, molecular mechanism underlying effect tomato on diabetes unclear. In this study, we focused anti-inflammatory compounds tomato. We found that extract plasma glucose inflammatory markers mice. screened fractions using...
A change in the free fatty acid (FFA) profile reflects an alteration lipid metabolism of peripheral tissue. high-throughput quantitative analysis method for individual FFAs therefore needs to be established. We report here optimized LC-MS assay a and high-sensitivity 10 major long-chain mouse plasma liver. This enables quantification by using trace amounts samples (2 µL mg liver tissue). apply this analyze FFA from obese model treated with bezafibrate, peroxisome proliferator-activated...
Peroxisome proliferator-activated receptor-α (PPARα) regulates lipid metabolism. We have reported that tomato fruit contains 9-Oxo-(10E,12E)-octadecadienoic acid (9-Oxo-(10E,12E)-ODA), a PPARα agonist. In this study, we found various samples contained 9-Oxo-(10E,12Z)-ODA and its 13-Oxo-ODA isomers. Furthermore, several isomers showed structural stability under hot acidic conditions.
Abstract The peroxisome proliferator‐activated receptor (PPAR)α is mainly expressed in the liver and plays an important role regulation of lipid metabolism. It has been reported that PPARα activation enhances fatty acid oxidation reduces fat storage. Therefore, agonists are used to treat dyslipidemia. In present study, we found 9‐oxo‐10( E ),12( Z ),15( )‐octadecatrienoic (9‐oxo‐OTA), which a α‐linolenic (ALA) derivative, tomato ( Solanum lycopersicum ) extract. We showed 9‐oxo‐OTA activated...
Abstract Peroxisome proliferator‐activated receptor (PPAR)γ is expressed in adipose tissue and plays a key role the regulation of adipogenesis. PPARγ activators are known to have potent antihyperglycemic activity used treat insulin resistance associated with diabetes. Therefore, many natural synthetic agonists treatment glucose disorders. In present study, we found that 13‐oxo‐9( Z ),11( E ),15( )‐octadecatrienoic acid (13‐oxo‐OTA), linolenic derivative, extract tomato ( Solanum lycopersicum...
Browning of adipose tissue is induced by specific stimuli such as cold exposure and consists up-regulation thermogenesis in white tissue. Recently, it has emerged an attractive target for managing obesity humans. Here, we performed a comprehensive analysis to identify genes associated with browning murine We focused on glycerol kinase (GYK) because its mRNA expression pattern highly correlated that uncoupling protein 1 (UCP1), which regulates the thermogenic capacity adipocytes. Cold...
Soybeans (Glycine max (L,) Merr,) contain γ-glutamyl peptides and oligosaccharides, these components play an important role in imparting the "kokumi" taste to foods. To gain insight into genetic diversities molecular mechanisms of accumulation oligosaccharides soybean, we measured contents using Japan World mini core collections. Similar other previously reported traits, wide variations were detected among accessions collections with respect content oligosaccharides. We found a positive...
While β-cryptoxanthin is hypothesized to have a preventive effect on lifestyle-related diseases, its underlying mechanisms are unknown. We investigated the of energy metabolism in adipose tissues and mechanism. C57BL/6J mice were fed high-fat diet (60% kcal fat) containing 0 or 0.05% for 12 weeks. treatment was found reduce body fat gain plasma glucose level, while increasing expenditure. The expression uncoupling protein (UCP) 1 elevated group. Furthermore, vivo assays showed that Ucp1 mRNA...
The high thermogenic activity of brown adipose tissue (BAT) has received considerable attention. Here, we demonstrated the role mevalonate (MVA) biosynthesis pathway in regulation adipocyte development and survival. inhibition 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), rate-limiting enzyme MVA molecular target statins, suppressed differentiation by suppressing protein geranylgeranylation-mediated mitotic clonal expansion. BAT neonatal mice exposed to statins during fetal period was...