A5085485237- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- Cancer Genomics and Diagnostics
- Renal cell carcinoma treatment
- Cancer-related molecular mechanisms research
- Prostate Cancer Diagnosis and Treatment
- MicroRNA in disease regulation
- Renal and related cancers
- RNA modifications and cancer
- Bladder and Urothelial Cancer Treatments
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- Cancer, Lipids, and Metabolism
- Cell Adhesion Molecules Research
- Ovarian cancer diagnosis and treatment
- Gastric Cancer Management and Outcomes
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- BRCA gene mutations in cancer
- Rheumatoid Arthritis Research and Therapies
- Economic and Financial Impacts of Cancer
- Breast Cancer Treatment Studies
- Liver Disease Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Vitamin D Research Studies
Vilnius University
2016-2025
National Cancer Institute
2016-2025
VIB-KU Leuven Center for Cancer Biology
2021
Natural (Czechia)
2008
Finnish Institute of Occupational Health
2003-2007
Abstract International differences in the incidence of many cancer types indicate existence carcinogen exposures that have not yet been identified by conventional epidemiology make a substantial contribution to burden 1 . In clear cell renal carcinoma, obesity, hypertension and tobacco smoking are risk factors, but they do explain geographical variation its 2 Underlying causes can be inferred sequencing genomes cancers from populations with different rates detecting patterns somatic...
In this paper, the utility of urine-circulating microRNAs (miRNAs) as potential biomarker prostate cancer (PCa), second most prevalent male worldwide, was evaluated. Cancerous (N=56) and non-cancerous (N=16) tissues were analysed on TaqMan Low Density Array, with initial screening 754 miRNAs in a subset samples. The abundance selected urine specimens from two independent cohorts patients PCa (N=215 overall), benign prostatic hyperplasia (BPH; N=23), asymptomatic controls (ASC; N=62) by means...
Background and Objectives: Rheumatoid arthritis (RA) is a chronic, inflammatory, autoimmune, multi-factorial disease, in which environmental genetic factors play major role. RA possibly linked to vitamin D deficiency receptor (VDR) gene polymorphisms, research demonstrates that FokI variant susceptibility associated with increased disease risk among Caucasians. The aim of this study was evaluate prevalence its correlation clinical parameters, determine the possible association VDR...
ATP-binding cassette (ABC) transporters are transmembrane proteins responsible for the efflux of a wide variety substrates, including steroid metabolites, through cellular membranes. For better characterization role ABC in prostate cancer (PCa) development, profile transporter gene expression was analyzed PCa and noncancerous tissues (NPT). TaqMan Low Density Array (TLDA) human plates were used profiling 10 6 NPT specimens. ABCB1 transcript level evaluated larger set cases (N = 78) 15) by...
Abstract The European Union (EU) initiative on the Digital Transformation of Health and Care (Digicare) aims to provide conditions necessary for building a secure, flexible, decentralized digital health infrastructure. Creating Research Innovation Cloud (HRIC) within this environment should enable data sharing analysis research across EU, in compliance with protection legislation while preserving full trust participants. Such HRIC learn from build existing infrastructures, integrate best...
Clear cell renal carcinoma (ccRCC) is the most prevalent form of cancer, accounting for over 75% cases. The asymptomatic nature disease contributes to late-stage diagnoses and poor survival. Highly vascularized immune infiltrated microenvironment are prominent features ccRCC, yet interplay between vasculature cells, progression response therapy remains poorly understood. Using droplet-based single-cell RNA sequencing we profile 50,236 transcriptomes from paired tumor healthy adjacent kidney...
The human epidermal growth factor receptor 2 (HER2) is an established biomarker for management of patients with breast cancer. While conventional testing HER2 protein expression based on semi-quantitative visual scoring the immunohistochemistry (IHC) result, efforts to reduce inter-observer variation and produce continuous estimates IHC data are potentiated by digital image analysis technologies. was performed tissue microarrays (TMAs) 195 early ductal carcinoma breast. Digital images slides...
Molecular studies of breast cancer revealed biological heterogeneity the disease and opened new perspectives for personalized therapy. While multiple gene expression-based systems have been developed, current clinical practice is largely based upon conventional pathologic criteria. This gap may be filled by development combined multi-IHC indices to characterize behaviour tumours. Digital image analysis (DA) with multivariate statistics data opens opportunities in this field. Tissue...
Prostate cancer (PCa) is the second most prevalent oncologic disease among men worldwide. Expression of various transcripts, including miRNAs, markedly deregulated in cancerous prostate tissue. This study aimed at identifying a PCa-specific expression profile miRNAs for subsequent use noninvasive diagnostics. MiRNA was profiled 13 PCa tissues using human miRNA microarrays. Highly expressed were selected analysis urine patients with (N = 143) and benign hyperplasia (BPH; N 23) by means real...
Previous studies have revealed a robust association between exposure to asbestos and human lung cancer. Accumulating evidence has highlighted the role of epigenome deregulation in mechanism carcinogen‐induced malignancies. We examined impact on DNA methylation. Our genome‐wide (using Illumina HumanMethylation450K BeadChip) cancer tissue paired normal from 28 asbestos‐exposed or non‐exposed patients, mostly smokers, distinctive methylation changes. identified number differentially methylated...
Patients with prostate cancer who have biochemical recurrence after curative therapy are at higher risk for distant metastasis and specific death. Assessment of aberrant DNA methylation in urine might complement currently used clinical prognostic factors serve as a noninvasive tool early prediction radical prostatectomy.Promoter 7 genes was evaluated by sensitive polymerase chain reaction 149 tissues, 37 noncancerous tissues 17 benign prostatic hyperplasia samples. Quantitative analysis the...
Only a part of prostate cancer (PCa) patients has aggressive malignancy requiring adjuvant treatment after radical prostatectomy (RP). Biomarkers capable to predict biochemical PCa recurrence (BCR) RP would significantly improve preoperative risk stratification and decisions. MicroRNA (miRNA) deregulation recently emerged as an important phenomenon in tumor development progression, however, the mechanisms remain largely unstudied. In present study, based on microarray profiling DNA...
Cutaneous melanoma, an aggressive malignancy, has undergone significant transformation in clinical management with the introduction of immune checkpoint inhibitors (ICIs) and targeted therapies. Current monitoring methods, such as imaging scans, present limitations, prompting exploration alternative biomarkers. This review comprehensively explores role circulating tumor DNA (ctDNA) advanced covering technical aspects, detection its prognostic predictive value. Recent findings underscore...
Abstract Hypermethylation of cytosines in CpG‐rich islands the promoter regions regulatory genes has been discovered as a common mechanism gene silencing during carcinogenesis. We analysed 64 primary lung carcinomas for methylation tumour suppressor (TSGs) p16 ( INK4a / CDKN2A ) and p14 ARF by methylation‐specific PCR, order to evaluate aberrant potential biomarker epigenetic alterations tobacco‐related cancer. Methylation was observed 34% (22/64) tumours examined. In particular, occurred...
Abstract Several chromosomal regions are recurrently amplified or deleted in lung tumors, but little is known about the underlying genes, which could be important mediators tumor formation progression. In cancer, RB1–CCND1–CDKN2A pathway, involved G1–S transition, damaged nearly all tumors. present study, we localized a novel amplicon tumors to fragment of less than 0.5 Mb at 12q13.3–q14.1 by using comparative genomic hybridization (CGH) on cDNA microarrays. This approach enabled us identify...
<i>Aims:</i> Superficial bladder cancer is a highly recurrent disease, with progression to muscle invasiveness occurring in 15–30% of cases. Promoter hypermethylation panel tumour suppressor genes involved cell cycle control, apoptosis and DNA repair was analyzed superficial tumours order evaluate the suitability epigenetic biomarkers for an earlier prediction aggressive course disease. <i>Method:</i> <i>p16, RAR</i>β, <i>RASSF1A, DAPK, </i>and...
Epigenetic differences are a common feature of many diseases, including cancer, and disease-associated changes have even been detected in bodily fluids. DNA modification studies circulating (cirDNA) may lead to the development specific non-invasive biomarkers. To test this hypothesis, we investigated cirDNA modifications prostate cancer patients with locally confined disease (n = 19), benign hyperplasias 20) men without any known 20). This initial discovery screen identified 39 modification,...
Background MicroRNAs (miRNAs) are widely studied non-coding RNAs that modulate gene expression. MiRNAs deregulated in different tumors including gastric cancer (GC) and have potential diagnostic prognostic implications. The aim of our study was to determine miRNA profile GC tissues, followed by evaluation miRNAs plasma patients. Using available databases bioinformatics methods we also aimed evaluate target genes confirmed differentially expressed validate these findings tissues. Methods...
// Ugne Gyvyte 1, * , Simonas Juzenas Violeta Salteniene 1 Juozas Kupcinskas 2 Lina Poskiene 3 Laimutis Kucinskas Sonata Jarmalaite 4, 5 Kristina Stuopelyte Ruta Steponaitiene Georg Hemmrich-Stanisak 6 Matthias Hübenthal Alexander Link 7 Sabine Franke 8 Andre Dalia Pangonyte Vaiva Lesauskaite 9 Limas 2, # Jurgita Skieceviciene Institute for Digestive Research, Academy of Medicine, Lithuanian University Health Sciences, Kaunas, Lithuania Department Gastroenterology, Pathological Anatomy, 4...
Significant numbers of prostate cancer (PCa) patients experience tumour upstaging and upgrading in surgical specimens that cause serious problems timely proper selection the treatment strategy. This study was aimed at evaluation a set established epigenetic biomarkers as noninvasive tool for more accurate PCa categorization before radical prostatectomy (RP). Quantitative methylation-specific PCR applied methylation analysis RARB, RASSF1, GSTP1 514 preoperatively collected voided or...
The molecular diversity of prostate cancer (PCa) has been demonstrated by recent genome-wide studies, proposing a significant number different markers. However, only few them have transferred into clinical practice so far. present study aimed to identify and validate novel DNA methylation biomarkers for PCa diagnosis prognosis. Microarray-based methylome data well-characterized cancerous noncancerous tissue (NPT) pairs was used the initial screening. Ten protein-coding genes were selected...