A5060616422- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- CRISPR and Genetic Engineering
- Cell death mechanisms and regulation
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Insect Utilization and Effects
- Histone Deacetylase Inhibitors Research
- Invertebrate Immune Response Mechanisms
- Ferroptosis and cancer prognosis
- BRCA gene mutations in cancer
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Photosynthetic Processes and Mechanisms
- Cancer Immunotherapy and Biomarkers
- RNA modifications and cancer
- Wnt/β-catenin signaling in development and cancer
- Neurobiology and Insect Physiology Research
- Chromosomal and Genetic Variations
- Cancer Genomics and Diagnostics
- Protein Degradation and Inhibitors
- DNA and Nucleic Acid Chemistry
Advanced Centre for Treatment, Research and Education in Cancer
2019-2022
Homi Bhabha National Institute
2019-2022
Indian Institute of Science Education and Research Pune
2017-2021
Tata Memorial Hospital
2019-2020
Indiana University Bloomington
2014
Johnson University
2011
Rutgers Cancer Institute of New Jersey
2011
Rutgers, The State University of New Jersey
2006-2010
Syracuse University
2008-2010
Maharaja Sayajirao University of Baroda
1999
Using an antibody against the phosphorylated form of His2Av (γ-His2Av), we have described time course for series events leading from formation a double-strand break (DSB) to crossover in Drosophila female meiotic prophase. MEI-P22 is required DSB and localizes chromosomes prior γ-His2Av foci. females, however, are among group organisms where synaptonemal complex (SC) not dependent on DSBs. In absence two SC proteins, C(3)G C(2)M, number DSBs oocytes significantly reduced. This consistent...
Initiation of DNA replication at origins more than once per cell cycle results in rereplication and has been implicated cancer. Here we use Drosophila to examine the checkpoint responses a developmental context. We find that increased Double-parked (Dup), ortholog Cdt1, damage. In most cells, this triggers caspase activation apoptotic death mediated by both p53-dependent -independent pathways. Elevated Dup also caused damage endocycling which switch G/S during normal development, indicating...
Apoptotic cell death is an important response to genotoxic stress that prevents oncogenesis. It known tissues can differ in their apoptotic response, but molecular mechanisms are little understood. Here, we show Drosophila polyploid endocycling cells (G/S cycle) repress the DNA damage through at least two mechanisms. First, expression of all p53 protein isoforms strongly repressed a post-transcriptional step. Second, p53-regulated pro-apoptotic genes epigenetically silenced cells, preventing...
The endocycle is a variant cell cycle comprised of alternating gap (G) and DNA synthesis (S) phases (endoreplication) without mitosis (M), which results in polyploidy large size. Endocycles occur widely nature, but much remains to be learned about the regulation this modified cycle. Here, we compared gene expression profiles mitotic cycling larval brain disc cells with endocycling fat body salivary gland Drosophila larva. indicated that many genes are positively regulated by heterodimeric...
BCCIP is a BRCA2- and CDKN1A(p21)-interacting protein that has been implicated in the maintenance of genomic integrity. To understand vivo functions BCCIP, we generated conditional knockdown transgenic mouse model using Cre-LoxP mediated RNA interference. The embryos displayed impaired cellular proliferation apoptosis at day E7.5. Consistent with these results, vitro blastocysts embryonic fibroblasts (MEFs) mice were considerably. deficient die before E11.5 day. Deletion p53 gene could not...
Abstract Special AT-rich binding protein-1 (SATB1) integrates higher-order chromatin architecture with gene regulation, thereby regulating multiple signaling pathways. In mammalian cells SATB1 directly interacts β-catenin and regulates the expression of Wnt targets by to their promoters. Whether Wnt/wg recruitment and/or its interactions other components remains elusive. Since Wnt/Wg is conserved from invertebrates humans, we investigated functions in regulation using cell-lines Drosophila ....
The gastrointestinal tract in metazoans consists of diverse epithelial cells with distinct cell morphology and physiological functions. development homeostasis epithelia involve spatiotemporal regulation by many signaling pathways, essential to confer their region-specific function identity. adult Drosophila midgut the mammalian intestine share a high degree conservation between such pathways. Due availability sophisticated techniques for genetic manipulation, is an excellent model study...
DNA replication stress is characterized by impaired fork progression, causing some of the forks to collapse and form breaks. It a primary cause genomic instability leading oncogenic transformation. The repair‐independent functions proteins RAD51 BRCA2, which are involved in homologous recombination (HR)‐mediated repair, crucial for protecting nascent strands from nuclease‐mediated degradation. BRCA2 CDKN1A‐interacting protein (BCCIP) associates with during HR‐mediated repair. Here, we...
Abstract Cancer immunotherapy with immune checkpoint inhibitors (ICI) has shown durable clinical benefits in patients immune-hot tumors. However, the majority of cancers are characterized by an immune-cold tumor microenvironment, which refractory to ICI therapy. Targeting synthetic vulnerabilities due genomic alterations is another promising approach that proven effective clinic (e.g. PARP BRCA-mutant tumors). Here, we aimed identify therapeutic targets have dual liabilities cell survival...