A5078870251- Fetal and Pediatric Neurological Disorders
- Cerebrospinal fluid and hydrocephalus
- Vibrio bacteria research studies
- Gut microbiota and health
- Cytomegalovirus and herpesvirus research
- MicroRNA in disease regulation
- Yersinia bacterium, plague, ectoparasites research
- Rabies epidemiology and control
- Infectious Encephalopathies and Encephalitis
- Neonatal and Maternal Infections
- Neonatal and fetal brain pathology
- Bacterial Infections and Vaccines
- Brain Tumor Detection and Classification
- Traumatic Brain Injury and Neurovascular Disturbances
- Neuroinflammation and Neurodegeneration Mechanisms
- Neonatal Health and Biochemistry
- Immune cells in cancer
- Parvovirus B19 Infection Studies
- Viral gastroenteritis research and epidemiology
- Oral microbiology and periodontitis research
- Reproductive tract infections research
- Genomics and Phylogenetic Studies
- Urinary Tract Infections Management
- Immune Response and Inflammation
- Bacterial Identification and Susceptibility Testing
Boston Children's Hospital
2023-2025
Harvard University
2023-2025
Penn State Milton S. Hershey Medical Center
2022-2023
Pennsylvania State University
2020-2023
Boston University
2023
Hershey (United States)
2021
Center for Disease Dynamics, Economics & Policy
2020
A new strain of the bacterium Paenibacillus contributes to postinfectious hydrocephalus in Ugandan infants.
Abstract Background We previously identified Paenibacillus species in the cerebrospinal fluid of 44% infants presenting for neurosurgical evaluation with findings consistent postinfectious hydrocephalus (PIH) Eastern Uganda. Here we sought to compare outcomes among hydrocephalic and without detection at time surgery. Methods In a prospective observational trial, 78 PIH who underwent (CSF) diversion had positive CSF polymerase chain reaction result (PP), 111 negative (PN). The primary outcome...
We previously identified Paenibacillus species in the cerebrospinal fluid of 44% infants presenting for neurosurgical evaluation with findings consistent postinfectious hydrocephalus (PIH) Eastern Uganda. Here we sought to compare outcomes among hydrocephalic and without detection at time surgery. In a prospective observational study, 78 PIH who underwent (CSF) diversion prior 90 days age had positive CSF polymerase chain reaction result (PP), 111 negative (PN). The primary outcome was...
The authors previously identified Paenibacillus species in the CSF of 44% infants presenting for neurosurgical evaluation with findings consistent postinfectious hydrocephalus (PIH) Eastern Uganda. Here, they sought to compare outcomes among hydrocephalic and without detection at time surgery. In a prospective observational study 189 PIH who underwent diversion prior 90 days age, 78 had positive polymerase chain reaction result (PP), 111 negative (PN). primary outcome was failure-free...
Abstract Background Paenibacillus thiaminolyticus may be an underdiagnosed cause of neonatal sepsis. Methods We prospectively enrolled a cohort 800 full-term neonates presenting with clinical diagnosis sepsis at 2 Ugandan hospitals. Quantitative polymerase chain reaction specific to P. and the genus were performed on blood cerebrospinal fluid (CSF) 631 who had both specimen types available. Neonates or species detected in either type considered potentially have paenibacilliosis, (37/631,...
Inflammation during neonatal brain infections leads to significant secondary sequelae such as hydrocephalus, which often follows sepsis in the developing world. In 100 African hydrocephalic infants we identified biological pathways that account for this response. The dominant bacterial pathogen was a Paenibacillus species, with frequent cytomegalovirus co-infection. A proteogenomic strategy employed confirm host immune response and define interplay within network. Immune activation...
The composition of the maternal vaginal microbiome influences duration pregnancy, onset labor, and even neonatal outcomes. Maternal research in sub-Saharan Africa has focused on non-pregnant postpartum microbiome. Here we aimed to illustrate relationship between 99 laboring Ugandan women intrapartum fever using routine microbiology 16S ribosomal RNA gene sequencing from two hypervariable regions (V1-V2 V3-V4). To describe microbes associated with microbial communities, pursued approaches:...
Individual and environmental health outcomes are frequently linked to changes in the diversity of associated microbial communities. Thus, deriving indicators based on microbiome measures is essential. While data generated using high-throughput 16S rRNA marker gene surveys appealing for this purpose, also generate a plethora spurious taxa.
Paenibacillus thiaminolyticus is a cause of postinfectious hydrocephalus among Ugandan infants. To determine whether spp pathogen in neonatal sepsis, meningitis, and hydrocephalus, we aimed to complete three separate studies The first study was on peripartum prevalence mother-newborn pairs. second assessed blood cerebrospinal fluid (CSF) from neonates with sepsis. third CSF infants hydrocephalus.In this observational study, recruited pairs without maternal fever (mother-newborn cohort),...
We have isolated a likely bacterial pathogen from cerebrospinal fluid Ugandan infant suffering hydrocephalus. Whole-genome sequencing and assembly of the genome clinical isolate, as well that previously deposited reference strain, identified isolate Paenibacillus thiaminolyticus, which has not been associated with widespread human infections.
We introduce mirTarRnaSeq, an R/Bioconductor package for quantitative assessment of miRNA-mRNA relationships within sample cohorts. mirTarRnaSeq is a statistical to explore predicted or pre-hypothesized following target prediction.We present two use cases applying mirTarRnaSeq. First, identify miRNA targets, we examined EBV miRNAs interaction with human and virus transcriptomes stomach adenocarcinoma. This revealed enrichment mRNA targets highly expressed in CD105+ endothelial cells,...
Hydrocephalus, the leading indication for childhood neurosurgery worldwide, is particularly prevalent in low- and middle-income countries. Hydrocephalus preceded by an infection, or postinfectious hydrocephalus, accounts up to 60% of hydrocephalus these areas. Since many children with suffer poor long-term outcomes despite surgical intervention, prevention remains paramount. Our previous studies implicated a novel bacterial pathogen, Paenibacillus thiaminolyticus, as causal agent neonatal...
To estimate the prevalence of cytomegalovirus (CMV) infections among newborn-mother pairs, neonates with sepsis, and infants hydrocephalus in Uganda.
. Hydrocephalus is the leading indication for pediatric neurosurgical care worldwide. Identification of postinfectious hydrocephalus (PIH) verses non-postinfectious hydrocephalus, as well pathogen involved in PIH crucial developing an appropriate treatment plan. Accurate identification requires clinical diagnosis by neuroscientists and microbiological analysis, which are time-consuming expensive. In this study, we develop a domain enriched AI method computerized tomography (CT)-based...
Abstract Postinfectious hydrocephalus (PIH), often following neonatal sepsis, is the most common cause of pediatric world-wide, yet microbial pathogens remain uncharacterized. Characterization agents causing PIH would lead to an emphasis shift from surgical palliation cerebrospinal fluid (CSF) accumulation prevention. We examined blood and CSF 100 consecutive cases control non-postinfectious (NPIH) in infants Uganda. Genomic testing was undertaken for bacterial, fungal, parasitic DNA, DNA...
The choroid plexus (ChP) is a vital brain barrier and source of cerebrospinal fluid (CSF). Here, we use chronic two-photon imaging in awake mice single-cell transcriptomics to demonstrate that addition these roles, the ChP complex immune organ regulates inflammation. In mouse meningitis model, neutrophils monocytes accumulated stroma surged across epithelial into CSF. Bi-directional recruitment from periphery and, unexpectedly, macrophages CSF helped eliminate repair barrier. Transcriptomic...
Background: Paenibacillus thiaminolyticus was identified as an important contributor to postinfectious hydrocephalus (PIH) among Ugandan infants a sequela from prior neonatal sepsis (NS). To better determine the significance of this organism’s role in NS and PIH, we complete separate studies with hydrocephalus, without evidence infection (case-control), well neonates (observational cohort), maternal-newborn pairs (case-control, maternal fever). Methods: From 2016-2019, 400 were recruited....
Background: Congenital cytomegalovirus (CMV) infection is the leading acquired cause of mental retardation, developmental delay and sensorineural deafness, yet a reliable assessment global disease burden lacking. Therefore, we estimate birth prevalence annual new cases congenital CMV (cCMV) worldwide. Methods: We performed systematic literature review meta-analysis to cCMV by World Health Organization (WHO) region Bank income level using MEDLINE/PubMed, Scopus, Cochrane Database Systematic...