A5101988776- Circular RNAs in diseases
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Neuroinflammation and Neurodegeneration Mechanisms
- Angiogenesis and VEGF in Cancer
- T-cell and B-cell Immunology
- Atherosclerosis and Cardiovascular Diseases
- Chemokine receptors and signaling
- RNA Research and Splicing
- Transgenic Plants and Applications
- Colorectal Cancer Surgical Treatments
- Virus-based gene therapy research
- Single-cell and spatial transcriptomics
- Animal Virus Infections Studies
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Neural dynamics and brain function
- Inflammatory mediators and NSAID effects
- Cell Adhesion Molecules Research
- Neurogenesis and neuroplasticity mechanisms
- Coronary Interventions and Diagnostics
- Neuroscience and Neuropharmacology Research
- Reproductive System and Pregnancy
- Colorectal and Anal Carcinomas
China Animal Health and Epidemiology Center
2025
Ministry of Agriculture and Rural Affairs
2025
Boston Children's Hospital
2019-2024
Harvard University
2024
The First Affiliated Hospital, Sun Yat-sen University
2012-2022
Sun Yat-sen University
2012-2022
Shandong First Medical University
2020
Shandong Tumor Hospital
2020
Taishan Medical University
2019
Chiba University
1996
Aberrant vascular smooth muscle cell (VSMC) proliferation and migration contribute to the development of pathologies, such as atherosclerosis post-angioplasty restenosis. The aim this study was determine whether miR-22-3p plays a role in regulating human artery (HASMC) function neointima formation.Quantitative real-time PCR (qRT-PCR) fluorescence situ hybridization (FISH) were used detect expression arteries. Cell Counting Kit-8 (CCK-8) EdU assays performed assess proliferation, transwell...
NK1.1+ T [natural killer (NK) T] cells express an invariant cell antigen receptor alpha chain (TCR alpha) encoded by V 14 and J 281 segments in association with a limited number of betas, predominantly beta 8.2. Expression the 14/J 281, but not 1, TCR transgenic mice lacking endogenous expression blocks development conventional leads to preferential NK cells, suggesting prerequisite role development. In 8.2 B 3 mice, two different CD3 epsilon expressions, epsilon(dim) epsilon(high), can be...
Increasing evidence suggests that microRNAs (miRNAs) play important roles in impaired endothelial cell (EC) angiogenesis during aging. However, their exact the aging process remain unclear. We aimed to determine whether miRNAs cause defects ECs and uncover underlying mechanisms. To study miRNA-induced changes aging, we performed microarray analyses on arterial collected from young mice. Using qRT–PCR, showed microRNA-125a-5p (mir-125a-5p) expression was approximately 2.9 times higher old...
Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly contagious that poses significant threat to the global pig farming industry, resulting in substantial economic losses. However, owing high variability of PRRSV unclear mechanisms infection, there are currently no effective vaccines or drugs available for its prevention control. Our previous report revealed pathogenic (HP-PRRSV) requires FAK-PI3K-AKT signaling pathway facilitate entry into cells. In this study, we further...
Background/Aims: ALT1 is a novel long non-coding RNA derived from the alternatively spliced transcript of deleted in lymphocytic leukemia 2 (DLEU2). To date, biological roles human vascular endothelial cells have not been reported. Methods: was knocked down by siRNAs. Cell proliferation analyzed cck-8. The existence and sequence were identified 3’ rapid amplification cDNA ends. interaction between lncRNA proteins RNA-Protein pull assay, immunoprecipitation, mass spectrometry analysis....
Abstract The choroid plexus (ChP), located in each brain ventricle, produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier, but is under-characterized. Here, we combine single cell RNA-Seq spatial mapping of RNA proteins to construct an atlas ChP developing adult mouse brain. Each comprises epithelial, endothelial, mesenchymal, immune, neuronal, glial cells, with distinct subtypes, differentiation states anatomical locations. Epithelial, fibroblast, macrophage populations had...
Background. Aging leads to vascular endothelial cell senescence. Decreased expression of VEGFA and VEGFR2 plays a crucial role in impairing angiogenesis senescent cells. Noncoding RNAs, including circular RNAs (circRNAs) microRNAs (miRNAs), regulate proliferation, differentiation, apoptosis, migration participate the occurrence development diseases. However, mechanism noncoding age-related dysfunction remains unclear. Here, we aimed identify circRNA that is associated with VEGF/VEGFR2...
MicroRNA (miR)‑31 serves a key role in various biological processes, including tumor development, angiogenesis and inflammation. Whether miR‑31 is involved the pathological processes of arteriosclerosis obliterans (ASO) remains to be elucidated, as does mechanism regulation arterial smooth muscle cells (ASMCs). In present study, expression was detected by reverse transcription‑quantitative polymerase chain reaction situ hybridization, significantly upregulated human ASO walls compared with...
Age is an important factor in many cardiovascular diseases, which endothelial cells (ECs) play role. Circular RNAs (circRNAs) have been reported but their role ageing EC-related angiogenesis unclear. We aimed to identify a functional circRNA that regulates during and explore its specific mechanism. In this study, we searched for differentially expressed circRNAs old (OECs) young (YECs) by sequencing found circGSE1 was significantly downregulated OECs. Our study showed could promote the...
Long non‑coding RNAs (lncRNAs) affect atherosclerosis by regulating the physiological and pathological processes of endothelial cells; however, role lncRNA WEE2 antisense RNA 1 (WEE2‑AS1) in arteriosclerosis obliterans (ASO) is not completely understood. The present study aimed to explore function WEE2‑AS1 human vascular cells. results indicated that was significantly elevated plasma artery tissue samples patients with ASO compared healthy controls. fluorescence situ hybridization suggested...
In aging, the regulation of angiogenesis is a dynamic and complex process. We aimed to identify characterize microRNAs that regulate during aging. showed that, in response vascular endothelial senescence, microRNA-25-3p (miR-25-3p) plays role an angiogenic microRNA by targeting TULA-2 (T-cell ubiquitin ligand-2)/SYK (spleen tyrosine kinase)/VEGFR-2 (vascular growth factor receptor 2) signaling vitro vivo. Mechanistic studies demonstrated miR-25-3p inhibits TULA-2/SYK/VEGFR-2 pathway cells....
Abstract It is known that tumor growth, invasion, and metastasis are all dependent on angiogenesis. Persistent angiogenesis a characteristic of malignant tumors. Quantification neovascularization in tumors considered to be an important independent prognostic marker. Integrin αvβ3 plays role metastasis. Arginine‐glycine‐aspartic acid (RGD) peptides can specifically bind integrin αvβ3. Radiolabeled RGD molecular probes used visualize blood vessels, monitor their changes before after...
Abstract Background To evaluate the specificity of expression patterns microRNAs (miRNAs) in circulating CD4 + T cells aged patients with atherosclerosis obliterans (ASO). Methods A comprehensive miRNA study was conducted using a microarray isolated from peripheral blood mononuclear (PBMCs) 33 ASO and 24 healthy donors. t test used for statistical analysis, average linkage method hierarchical clustering. The results were validated by qRT–PCR. Putative targeted pathways associated miRNAs...
Objective: To investigate the effects of miR-106a-5p on proliferation and migration vascular endothelial cells, possible target gene in cells. Methods: Human umbilical vein cells (HUVEC) were cultured vitro, expression mir-106a-5p HUVECs was detected by real-time fluorescent quantitative polymerase chain reaction (RT-qPCR). Cell counting kit-8 (CCK-8) wound healing assays used to respectively.Dual luciferase repoter assay identify miR-106a-5p--STAT3. Results: inhibited function (F=13.62,...
Abstract Patients with locally advanced rectal cancer who achieve a pathological complete response (pCR) to neoadjuvant chemoradiation therapy (CRT) have an improved prognosis. The need for surgery in these patients has been questioned; however, the proportion of pCR and receive wait‐and‐watch strategy is small. This meta‐analysis aimed evaluate whether consolidation chemotherapy leads higher rate than standard CRT alone cancer. To this end, we carried out three clinical controlled trials,...