A5064564619- Pneumonia and Respiratory Infections
- Immune Cell Function and Interaction
- Microbial infections and disease research
- T-cell and B-cell Immunology
- Respiratory viral infections research
- CAR-T cell therapy research
- Colorectal Cancer Surgical Treatments
- Reproductive System and Pregnancy
- Antibiotics Pharmacokinetics and Efficacy
- Probiotics and Fermented Foods
- Bacterial Infections and Vaccines
- Diverticular Disease and Complications
- Music Therapy and Health
- Gastrointestinal disorders and treatments
- Enterobacteriaceae and Cronobacter Research
- Cancer Immunotherapy and Biomarkers
- Kawasaki Disease and Coronary Complications
- Polyomavirus and related diseases
- Coronary Artery Anomalies
- Colorectal and Anal Carcinomas
- Influenza Virus Research Studies
- Case Reports on Hematomas
- Potassium and Related Disorders
- Viral-associated cancers and disorders
- Metabolism and Genetic Disorders
Advanced Institute of Industrial Technology
2025
Kawasaki Medical School
2013-2021
Japanese Red Cross Narita Hospital
2005-2021
Obihiro National Hospital
2012
Chibaken Saiseikai Narashino Hospital
2004-2005
Social Welfare Organization Saiseikai Imperial Gift Foundation
2005
Chiba University
1995-2003
National Institute of Animal Health
2000
Kirin (Japan)
1997-1998
Japan Science and Technology Agency
1997
Natural killer T (NKT) lymphocytes express an invariant cell antigen receptor (TCR) encoded by the Valpha14 and Jalpha281 gene segments. A glycosylceramide-containing alpha-anomeric sugar with a longer fatty acyl chain (C26) sphingosine base (C18) was identified as ligand for this TCR. Glycosylceramide-mediated proliferative responses of NKT cells were abrogated treatment chloroquine-concanamycin or monoclonal antibodies against CD1d/Vbeta8, CD40/CD40L, B7/CTLA-4/CD28, but not interference...
A lymphocyte subpopulation, the V α 14 natural killer T (NKT) cells, expresses both NK1.1 and a single invariant cell receptor encoded by J 281 gene segments. Mice with deletion of segment were found to exclusively lack this subpopulation. The NKT cell–deficient mice could no longer mediate interleukin-12 (IL-12)–induced rejection tumors. Although antitumor effect IL-12 was thought be mediated through cells an essential target IL-12, they their cytotoxicity NK-like effector mechanism after...
We have recently identified alpha-galactosylceramide (alpha-GalCer) as a specific ligand for an invariant Valpha14/Vbeta8.2 T cell receptor exclusively expressed on the majority of Valpha14 NKT cells, novel subset lymphocytes. Here, we report that alpha-GalCer selectively activates cells resulting in prevention tumor metastasis. The effector mechanisms ligand-activated seem to be mediated by natural killer (NK)-like nonspecific cytotoxicity. Indeed, cytotoxic index obtained...
The immunological mechanisms that regulate abortion are largely unknown. Here, we found a distinct subset of lymphocytes, Vα14 NKT cells expressing an invariant antigen receptor encoded by Vα14/Jα281 and Vβ7 segments, accumulated in the decidua during pregnancy provoked upon stimulation with α-galactosylceramide (α-GalCer), specific ligand for cells. α-GalCer-mediated was not observed NKT-, IFN-γ-, tumor necrosis factor α-, or perforin-knock-out mice appeared to be due degeneration embryonic...
ABSTRACT We conducted nationwide surveillance to investigate regional differences in macrolide-resistant (MR) Mycoplasma pneumoniae strains Japan. The prevalence of MR M. pediatric patients gradually increased between 2008 and 2012. Although were observed, high levels genes detected all seven areas throughout Japan ranged from 50% 93%. These closely related the previous administration macrolides.
ABSTRACT The importance of macrolide-resistant (MR) Mycoplasma pneumoniae has become much more apparent in the past decade. We investigated differences therapeutic efficacies macrolides, minocycline, and tosufloxacin against MR M. . A total 188 children with pneumonia confirmed by culture PCR were analyzed. Of these, 150 patients had a strain an gene 134 one A-to-G mutation at position 2063 23S rRNA domain V. Azithromycin ( n = 27), clarithromycin 23), 62), or minocycline 38) was used for...
We evaluated isolates obtained from children with Mycoplasma pneumoniae infection throughout Japan during 2008-2015. The highest prevalence of macrolide-resistant M. was 81.6% in 2012, followed by 59.3% 2014 and 43.6% 2015. among has decreased.
NK1.1+ T [natural killer (NK) T] cells express an invariant cell antigen receptor alpha chain (TCR alpha) encoded by V 14 and J 281 segments in association with a limited number of betas, predominantly beta 8.2. Expression the 14/J 281, but not 1, TCR transgenic mice lacking endogenous expression blocks development conventional leads to preferential NK cells, suggesting prerequisite role development. In 8.2 B 3 mice, two different CD3 epsilon expressions, epsilon(dim) epsilon(high), can be...
<i>Background/Aims:</i> To determine whether preoperative natural killer (NK) cell activity has any prognostic significance in colon cancer patients. <i>Methods:</i> The study population consisted of 140 patients with cancer. NK was determined within 2 weeks before surgery 128 and at the time diagnosis remaining 12 who either did not undergo or underwent palliative only. Disease progression postoperative prognosis were examined relation to activity....
IntroductionMycoplasma pneumoniae contributes to numerous pneumonia cases among children and young adults. Therefore, this study aimed investigate the prevalence of M. infections Japanese children, occurring since 2008.MethodsNasopharyngeal swab specimens were obtained from all cases, following which real-time PCR was performed identify pneumoniae. Further, p1 genotypes isolates determined using restriction fragment length polymorphism typing method.ResultsThe annual rate macrolide-resistant...
Vα14 NKT cells express an invariant antigen receptor encoded by and Jα281 gene segments as well natural killer (NK) markers, including NK1.1. Here, we describe a precursor population of (pre-NKT) that expresses NK1.1, T cell β, pTα, RAG1/2 but not surface CD3ɛ. Such pre-NKT were differentiated successfully in vitro into mature CD3ɛ + IL-15 granulocyte/macrophage colony-stimulating factor (GM-CSF) conjunction with stroma cells. Interestingly, only GM-CSF without induced the Vα14-Jα281...
We compared the antimicrobial susceptibility of Mycoplasma pneumoniae isolates from pediatric patients in Japan 2011-2012 and 2015-2016, when epidemics occurred. The activity macrolides tetracyclines against M. infection tended to be restored 2015-2016. There was no change quinolones infection.
Isolated hypoaldosteronism is a rare and occasionally life-threatening cause of salt wasting in infancy. A 2-month-old Japanese boy unrelated parents was examined for failure to thrive poor weight gain. Laboratory findings were hyponatremia, hyperkalemia, high plasma renin low aldosterone levels. Spot urine analysis by gas chromatography-mass spectrometry (GC-MS) showed that urinary excretion corticosterone metabolites elevated. Whereas 18-hydroxycortricosterone within the normal range,...
ObjectiveChlamydia pneumoniae and Mycoplasma are both common causes of atypical pneumonia. We conducted an annual national survey Japanese children to screen them for C. infections during the M. epidemic season.MethodsNasopharyngeal swab specimens were collected from aged 0–15 years with suspected acute lower respiratory tract infection due pathogens, at 85 medical facilities in Japan June 2008 March 2018. Specimens tested using real-time polymerase chain reaction assays.ResultsOf 5002...
Abstract USP9X variants have been reported in patients with X-linked intellectual disability. Here, we report two female disability and pigment abnormalities along Blaschko lines. Targeted resequencing identified novel heterozygous variants, c.4068_4072del (p. (Leu1357Tyrfs*12)) c.1201C>T (Arg401*)), . Our findings provide further evidence that cause
Despite the fact that accessible digital musical instruments can take into account level of cognitive demands, previous studies have been conducted with patients mild impairment (MCI), and it is not known whether they be used by people moderate to severe dementia or worsening behavioral psychological symptoms (BPSD). The participant was an 88-year-old woman vascular (VaD) (Mini-Mental State Examination [MMSE] Neuropsychiatric Inventory [NPI] scores: 8 20, respectively). Music therapy (MT)...
Recombination-activating gene (RAG) 1 and 2 are essential for the rearrangement of antigen receptors both T B cells. To investigate RAG expression in peripheral lymphoid organs other than thymus bone marrow, we established mice which a green fluorescent protein (GFP) is knocked-in RAG2 locus (RAG2-GFP mice). In marrow heterozygous RAG2-GFP mice, as expected, GFP was detected appropriate stages developing Interestingly, only fraction Thy-1.2(+) cells Peyer's patch were found to be GFP(+)...
Although febrile neutropenia (FN) is one of the most common adverse events produced by chemotherapy, its microbiological etiology determined for only 15% to 30% cases.We investigated rate viremia with DNA viruses in patients FN.From June 2012 April 2014, 72 blood samples from 24 receiving who experienced FN episodes, were examined presence herpes and other viruses. We used real-time polymerase chain reaction assays detect simplex virus type 1 2, varicella zoster virus, Epstein-Barr...
IntroductionThe features of pneumonia in children with neurologic impairment (NI) resemble those healthcare-associated is defined as occurring the community associated healthcare risk factors. There are currently no guidelines for treatment NI. Here, we assessed whether applicable treating adults could be applied to NI.MethodsBetween 2008 and 2019, enrolled NI who developed were treated pediatric ward Kawasaki Medical School Hospital. We evaluated patient characteristics, frequency isolation...
Infants ≤90 days old can exhibit non-specific signs of infection, even in cases serious bacterial infection (SBI).This prospective study included infants aged hospitalized for fever from June 2017 to August 2019. Nasopharyngeal swabs were tested using multiplex real-time polymerase chain reaction (PCR) tests and 16S ribosomal RNA analysis whole blood determine causative microorganisms. Data pertaining inflammatory markers, maximum body temperature (BT), respiratory symptoms their cohabiting...