A5059576586- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Ovarian function and disorders
- Estrogen and related hormone effects
- Reproductive Biology and Fertility
- Uterine Myomas and Treatments
- Gynecological conditions and treatments
- Hypothalamic control of reproductive hormones
- Reproductive Physiology in Livestock
- Endometrial and Cervical Cancer Treatments
- Birth, Development, and Health
- Pelvic floor disorders treatments
- Sperm and Testicular Function
- Reproductive Health and Contraception
- Sexual function and dysfunction studies
- Reproductive tract infections research
- Neuroendocrine regulation and behavior
- Hormonal and reproductive studies
- Adipose Tissue and Metabolism
- Fibroblast Growth Factor Research
- Pelvic and Acetabular Injuries
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Menopause: Health Impacts and Treatments
- Gut microbiota and health
- Protease and Inhibitor Mechanisms
Oregon National Primate Research Center
2016-2025
Oregon Health & Science University
2015-2025
Duke University
2023
University of North Carolina at Chapel Hill
2023
ZRT Laboratory
2020
University of Portland
2019
Advanced Imaging Research (United States)
2008
Bayer (Germany)
2007-2008
Providence College
2004
St Vincent Medical Center
2004
Abstract Pre-existing serum antibodies play an important role in vaccine-mediated protection against infection but the underlying mechanisms of immune memory are unclear. Clinical studies indicate that antigen-specific antibody responses can be maintained for many years, leading to theories reactivation/differentiation B cells into plasma is required sustain long-term production. Here, we present a decade-long study which demonstrate site-specific survival bone marrow-derived and durable...
Estrogen action is dependent upon the presence of specific ligand-activated receptors in target tissues. The aim present experiments was to compare spatial and temporal pattern expression estrogen receptor β (ERβ) with that ERα full thickness endometrial samples (from superficial basal zone) obtained from both women rhesus macaques. Immunohistochemical localization antibodies revealed ERβ were expressed nuclei glands stroma. Consistent previous studies, declined stroma functionalis during...
We have continued to explore the 3,3-dialkyl-5-aryloxindole series of progesterone receptor (PR) modulators looking for new agents be used in female healthcare: contraception, fibroids, endometriosis, and certain breast cancers. Previously we reported that subtle structural changes with this related templates produced functional switches between agonist antagonist properties ( Fensome et al. Biorg. Med. Chem. Lett. 2002, 12, 3487; 2003, 13, 1317 ). herein report a switch within...
In vitro studies have shown that keratinocyte growth factor (KGF, also known as FGF-7) is secreted by fibroblasts and mitogenic specifically for epithelial cells. Therefore, KGF may be an important paracrine mediator of cell proliferation in vivo. Because stromal cells are thought to influence glandular the primate endometrium, we investigated hormonal regulation cellular localization mRNA expression rhesus monkey uterus. Tissues were obtained both from naturally cycling monkeys follicular...
We tested the hypothesis that progesterone (P4) acts at a local level to inhibit luteal apoptosis. Initial experiments employed aminoglutethimide, P450 cholesterol side-chain cleavage inhibitor, steroid synthesis. Cultured bovine cells were treated with aminoglutethimide (0.15 mM) ± P4 (500 ng/ml) for 48 h. Luteal recovered and snap frozen isolation analysis of oligonucleosomal DNA fragmentation or fixed morphological analysis. Medium was collected levels by RIA. Aminoglutethimide inhibited...
Antiprogestins (APs) inhibit estradiol (E2)-stimulated endometrial growth in women and nonhuman primates, but the mechanism of this “antiestrogenic” action is unknown. Here, we report that APs up-regulate androgen receptor (AR) both macaques, an effect might play a role antiproliferative effects on primate endometrium. In addition, because there are discrepancies literature regulation localization AR endometrium, used situ hybridization immunocytochemistry to evaluate hormonal influences...
Abstract Endometriosis is a painful disorder where endometrium‐like tissue forms lesions outside of the uterine cavity. Intraoperative identification and removal these are difficult. This study presents nanoplatform that concurrently delineates ablates endometriosis tissues using real‐time near‐infrared (NIR) fluorescence photothermal therapy (PTT). The consists dye, silicon naphthalocyanine (SiNc), capable both NIR imaging PTT, polymeric nanoparticle as SiNc carrier to following systemic...
Abstract Endometriosis is a devastating disease in which endometrial‐like tissue forms lesions outside the uterus. It causes infertility and severe pelvic pain ≈176 million women worldwide, there currently no cure for this disease. Magnetic hyperthermia could potentially eliminate widespread endometriotic but has not previously been considered treatment because conventional magnetic nanoparticles have relatively low heating efficiency can only provide ablation temperatures (>46 °C)...
Determination of the mitotic index in sections endometrium stained with haematoxylin and eosin (H&E) is difficult time-consuming. We assessed value two markers as immunocytochemical reagents for measuring rates endometrium.Mitotic protein monoclonal antibody 2 (MPM-2) anti-phosphorylated histone H3 (Phospho H3) were applied to paraffin rhesus macaque human endometrium.In estrogen-treated mean +/- SEM indices were: H&E 1.5 0.25%, Phospho 1.02 0.23% MPM-2 0.69 0.17%; these not statistically...
The differential association of hypoandrogenism in men and hyperandrogenism women with insulin resistance obesity suggests that androgens may exert sex-specific effects on adipose other tissues, although the underlying mechanisms remain poorly understood. Moreover, recent studies also suggest rodents humans respond differently to androgen imbalance. To achieve better insight into clinically relevant action, we used nonhuman primates investigate direct gonadectomy hormone replacement white...
We previously reported that RU486 can reverse progesterone (P)-induced suppression of the estrogen receptor (ER) in uterus pregnant rhesus monkeys, but we did not determine whether (E) could act through its presence P and RU486. To pursue this question, treated spayed monkeys with various hormonal regimens evaluated effects E oviduct endometrium, without treatment, on ER progestin (PR) levels, morphology, apoptosis, degree proliferation. The latter was assessed immunocytochemically a...
Matrix metalloproteinases (MMPs) are highly expressed in the human endometrium during menstruation, and these enzymes participate cyclic destruction regeneration characteristic of primate endometrium. To examine hormonal regulation MMPs vivo, we evaluated MMP expression localization ovariectomized rhesus macaques under various conditions. Although all were up-regulated by progesterone (P4) withdrawal, their declined spontaneously after menstruation absence P4. Of 7 examined, only matrilysin...
The long-term effects of the antiprogestin ZK 137 316 on reproductive tract morphology in rhesus macaques were investigated. monkeys injected daily (i.m.) for five menstrual cycles with vehicle or 0.01, 0.03 0.1 mg 316/kg body weight. Reproductive tracts (n = 3/ group) collected during mid-luteal phase (day 8) fifth cycle control, 0.01 and mg/kg groups, 6–7 days after oestradiol peak group. treatment resulted a dose-dependent atrophy endometrium, marked by reduced mitotic activity glands,...
The hormonally driven expression and cell-specific localization patterns of the progesterone receptor membrane components (PGRMC1 PGRMC2) in macaque endometrium during menstrual cycle are unknown. Additionally, PGRMC1 PGRMC2 secretory eutopic primates afflicted with endometriosis also Therefore, we used real-time PCR to quantify transcript levels PGRMCs well-defined samples collected from artificially cycled macaques cycle, phase naturally cycling endometriosis. In situ hybridization...
Recently, we reported that in rhesus monkeys, RU 486 treatment could inhibit the ability of estradiol (E2) to stimulate endometrial regeneration without inhibiting E2-dependent oviductal regeneration. In work, had been administered at end an artificial luteal phase when oviducts were regressed, endometrium was a late secretory state, and estrogen progestin receptors (ER PR, respectively) minimal levels both organs. current after 2 weeks priming fully differentiated, proliferative ER PR...
Abstract Several reports indicate that vascular endothelial growth factor (VEGF) expression is increased in endometrial glands and stroma during the menstrual phase human endometrium. Here we report VEGF receptor type 2 (KDR), normally expressed only endothelium, was dramatically up-regulated stromal cells of superficial zones premenstrual both macaque This increase detectable by Northern analysis, situ hybridization, immunocytochemistry cell specific, zone cycle specific. That is, it...
Does chronic hyperandrogenemia beginning at menarche, in the absence and presence of a western-style diet (WSD), alter ovarian uterine structure-function young adult rhesus monkeys?Phenotypic alterations structure/function were induced by exogenous testosterone (T), compounded WSD (T+WSD).Hyperandrogenemia is well-established component PCOS observed adolescent girls, indicating potential pubertal onset disease symptoms. Obesity often associated with it hypothesized that metabolic dysfunction...
According to current theory, endometriosis is initiated during retrograde menstruation when menstrual fragments flow out of the fimbriated end fallopian tubes and become established on ovarian surface or other sites in peritoneal cavity. In recent years, new data have accumulated properties menstruating tissue itself, several laboratories agree that this rich matrix metalloproteinases (MMPs) may facilitate endometriotic implantation. Recently, we found vascular endothelial growth factor...