A5021175186- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- Advanced Electron Microscopy Techniques and Applications
- Statistical Methods and Inference
- Random lasers and scattering media
- Blood groups and transfusion
- Simulation Techniques and Applications
- Chronic Kidney Disease and Diabetes
- Gene Regulatory Network Analysis
- Cancer, Hypoxia, and Metabolism
- Cancer Genomics and Diagnostics
- Ion Transport and Channel Regulation
- MRI in cancer diagnosis
- HIV/AIDS drug development and treatment
- Theoretical and Computational Physics
- Renal and related cancers
- Renal Diseases and Glomerulopathies
- Metabolism and Genetic Disorders
- Advanced biosensing and bioanalysis techniques
- Photoacoustic and Ultrasonic Imaging
- Glycosylation and Glycoproteins Research
Medical University of Vienna
2022-2024
Vienna Biocenter
2022-2024
Max Perutz Labs
2022-2024
RELX Group (United States)
2023
University of Vienna
2022
University of Göttingen
2021
Organoids enable in vitro modeling of complex developmental processes and disease pathologies. Like most 3D cultures, organoids lack sufficient oxygen supply therefore experience cellular stress. These negative effects are particularly prominent models, such as brain organoids, can affect lineage commitment. Here, we analyze organoid fetal single-cell RNA sequencing (scRNAseq) data from published new datasets, totaling about 190,000 cells. We identify a unique stress signature the all...
During brain development, neural progenitors expand through symmetric divisions before giving rise to differentiating cell types via asymmetric divisions. Transition between those modes varies among individual stem cells, resulting in clones of different sizes. Imaging-based lineage tracing allows for analysis at high cellular resolution but systematic approaches analyse clonal behaviour entire tissues are currently lacking. Here we implement whole-tissue by genomic DNA barcoding 3D human...
With recent advances in multi-color super-resolution light microscopy, it is possible to simultaneously visualize multiple subunits within biological structures at nanometer resolution. To optimally evaluate and interpret spatial proximity of stainings on such an image, colocalization analysis tools have be able integrate prior knowledge the local geometry recorded complex. We present MultiMatch analyze abundance location chain-like particle arrangements microscopy based multi-marginal...
Individuals of African ancestry carrying two pathogenic variants apolipoprotein 1 (APOL1) have a substantially increased risk for developing chronic kidney disease. The course APOL1 nephropathy is extremely heterogeneous and shaped by systemic factors such as response to interferon. However, additional environmental operating in this second-hit model been less well defined. Here, we reveal that stabilization hypoxia-inducible transcription (HIF) hypoxia or HIF prolyl hydroxylase inhibitors...
Human brain organoids are powerful in vitro models for development and disease. However, their variability can complicate use biomedical research drug discovery. Both the specific protocol as well pluripotent starting cell line influence organoid result incomplete representation of types an experiment. Here, we systematically analyze cellular transcriptional landscape grown from multiple lines using four different protocols recapitulating dorsal ventral forebrain, midbrain, striatum. We...
Abstract Organoids enable disease modeling in complex and structured human tissue, vitro . Like most 3D models, they lack sufficient oxygen supply, leading to cellular stress. These negative effects are particularly prominent like brain organoids, where can prevent proper lineage commitment. Here, we analyze organoid fetal single cell RNA sequencing (scRNAseq) data from published new datasets totaling over 190,000 cells. We describe a unique stress signature found all samples, but not...
Time-resolved sensing of fluorescence quanta provides exceptionally versatile information–including access to nanoscopic structure, chemical environment and nonclassical behavior quantum emitters. Combined spectro-temporal information is typically obtained using spatial dispersion with photoelectron imaging such as streak-cameras or position-sensitive counting and, alternatively, sequential filtering single-pixel detectors. However, schemes require complex, expensive low-sensitivity...
The interplay between genetic and environmental factors influences the course of chronic kidney disease (CKD). In this context, alterations in gene MUC1 (Mucin1) predispose to development CKD. These variations comprise polymorphism rs4072037, which alters splicing mRNA, length a region with variable number tandem repeats (VNTR), rare autosomal-dominant inherited dominant-negative mutations or 5' VNTR that causes autosomal dominant tubulointerstitial (ADTKD-MUC1). As hypoxia plays pivotal...
Abstract With recent advances in multi-color super-resolution light microscopy it has become possible to simultaneously visualize multiple subunits within complex biological structures at nanometer resolution. To opti-mally evaluate and interpret spatial proximity of stainings on such an image, colocalization analysis tools have be able integrate prior knowledge the local geometry recorded complex. Here, we present MultiMatch analyze abundance location chain-like particle arrangements based...