A5003314346- Photosynthetic Processes and Mechanisms
- Micro and Nano Robotics
- CRISPR and Genetic Engineering
- Photoreceptor and optogenetics research
- Molecular Communication and Nanonetworks
- Genetic and Kidney Cyst Diseases
- Protist diversity and phylogeny
- Algal biology and biofuel production
- Cannabis and Cannabinoid Research
- Microtubule and mitosis dynamics
- Redox biology and oxidative stress
- Amino Acid Enzymes and Metabolism
- Genetics, Aging, and Longevity in Model Organisms
- Polyamine Metabolism and Applications
- Neonatal Respiratory Health Research
- Bacterial Genetics and Biotechnology
- Evolution and Genetic Dynamics
- Neural dynamics and brain function
- Congenital Diaphragmatic Hernia Studies
- Animal Genetics and Reproduction
- Biocrusts and Microbial Ecology
- Respiratory Support and Mechanisms
- Mitochondrial Function and Pathology
- Orbital Angular Momentum in Optics
- Innovation and Socioeconomic Development
Berliner Hochschule für Technik
2025
Humboldt-Universität zu Berlin
2017-2024
Freie Universität Berlin
2022-2024
Charité - Universitätsmedizin Berlin
2022-2024
RELX Group (United States)
2023
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2021
Ludwig-Maximilians-Universität München
2013
The fast-growing biflagellated single-celled chlorophyte
Standardized and well-characterized genetic building blocks are a prerequisite for the convenient reproducible assembly of novel modules devices. While numerous standardized parts exist Escherichia coli, such tools still missing Gram-positive model organism Bacillus subtilis. The goal this study was to develop thoroughly evaluate toolbox.We developed five BioBrick-compatible integrative B. subtilis vectors by deleting unnecessary removing forbidden restriction sites allow cloning in BioBrick...
Abstract The green unicellular alga Chlamydomonas reinhardtii with two photoreceptors called channelrhodopsins is a model organism that gave birth to new scientific field of biomedical studies, optogenetics. Although are helping decipher the activity human brain, their functionality has never been extensively studied in origin, mainly due difficulties connected reverse genetic interventions. In this study, we present CRISPR-Cas9-based technique enables precise vivo exchange single amino...
Abstract Although microalgae have only recently been recognized as part of the plant and soil microbiome, their application biofertilizers has a tradition in sustainable crop production. Under consideration ability to produce growth‐stimulating hormone cytokinin (CK), known also induce pathogen resistance, we assessed biocontrol CK‐producing microalgae. All pro‐ eukaryotic tested were able enhance tolerance tobacco against Pseudomonas syringae pv. tabaci ( PsT ) infection. Since...
Abstract Photoorientation in motile fungal zoospores is mediated by rhodopsin guanylyl cyclases (RGCs). In certain chytrids, these photoreceptors form heterodimers consisting of a visible-light-absorbing RGC paired with neorhodopsin (NeoR), distinguished its unique spectral properties: far-red absorption and high fluorescence. Leveraging the native fluorescence NeoR, we detected RGCs living fungus Rhizoclosmatium globosum . The reversible photoswitching bistable NeoR enabled super-resolution...
Abstract The use of CRISPR/Cas endonucleases has revolutionized gene editing techniques for research on Chlamydomonas reinhardtii. To better utilize the system, it is essential to develop a more comprehensive understanding DNA repair pathways involved in genome editing. In this study, we have analyzed contributions from canonical KU80/KU70-dependent nonhomologous end-joining (cNHEJ) and polymerase theta (POLQ)-mediated end joining SpCas9-mediated untemplated mutagenesis homology-directed...
For the unicellular alga Chlamydomonas reinhardtii, presence of N -glycosylated proteins on surface two flagella is crucial for both cell-cell interaction during mating and flagellar adhesion. However, it not known whether only or also composition -glycans attached to respective important these processes. To this end, we tested several C. reinhardtii insertional mutants a CRISPR/Cas9 knockout mutant xylosyltransferase 1A, all possessing altered -glycan compositions. Taking advantage atomic...
The dihydrolipoamide acetyltransferase subunit DLA2 of the chloroplast pyruvate dehydrogenase complex (cpPDC) in green alga Chlamydomonas reinhardtii has previously been shown to possess moonlighting activity gene expression. Under mixotrophic growth conditions, forms part a ribonucleoprotein particle (RNP) with psbA mRNA that encodes D1 protein photosystem II (PSII) reaction center. Here, we report on characterization molecular switch regulates shuttling between its functions carbon...
During gestation, the most drastic change in oxygen supply occurs with onset of ventilation after birth. As too early exposure premature infants to high arterial pressure leads characteristic diseases, we studied adaptation sensing system and its targets, hypoxia-inducible factor- (HIF-) regulated genes (HRGs) developing lung. We draw a detailed picture by integrating information from qPCR, immunoblotting, situ hybridization, single-cell RNA sequencing data ex vivo models. HIF1α protein was...
Microbial colonization of surfaces represents the first step towards biofilm formation, which is a recurring phenomenon in nature with beneficial and detrimental implications technological medical settings. Consequently, there interest elucidating fundamental aspects initial stages formation microorganisms on solid surfaces. While most research oriented to understand bacterial surface colonization, principles motile, photosynthetic microbes remain largely unexplored so far. Recent...
Abstract Calredoxin (CRX) is a calcium (Ca2+)-dependent thioredoxin (TRX) in the chloroplast of Chlamydomonas (Chlamydomonas reinhardtii) with largely unclear physiological role. We elucidated CRX functionality by performing in-depth quantitative proteomics wild-type cells compared crx insertional mutant (IMcrx), two CRISPR/Cas9 KO mutants, and rescues. These analyses revealed that NADPH-dependent TRX reductase (NTRC) co-regulated CRX. Electron transfer measurements inhibits reduction...
The primary cilium is a cellular compartment specialized for receipt of extracellular signals that essential development and homeostasis. Although intraciliary responses to engagement ciliary receptors are well studied, fundamental questions remain about the mechanisms molecules transduce into in cytoplasm. During fertilization bi-ciliated alga Chlamydomonas reinhardtii, adhesion between plus minus gametes triggers an immediate ∼10-fold increase cAMP consequent cytoplasm required cell-cell...
The polyamines putrescine, spermidine and spermine are organic polycations that regulate many cell functions including proliferation differentiation. It is known certain genes of the polyamine system dysregulated after kidney ischemia reperfusion injury. Here we examined hypothesis different forms acute chronic injury lead to similar changes in expression patterns system. In models involved homeostasis were analyzed by RT-qPCR RNAScope. these models, catabolic enzymes (Aoc1 Sat1) was...
Abstract For the unicellular alga Chlamydomonas reinhardtii , presence of N -glycosylated proteins on surface two flagella is crucial for both cell-cell interaction during mating and flagellar adhesion. It unknown whether composition -glycans attached to respective important these processes. To this end, we examined several C. insertional mutants a CRIPSR/Cas9 knockout mutant xylosyltransferase 1A, all possessing altered -glycan compositions. Taking advantage atomic force microscopy...
Microbial colonization of surfaces represents the first step towards biofilm formation, which is a recurring phenomenon in nature with beneficial and detrimental implications technological medical settings. Consequently, there current interest elucidating fundamental aspects initial stages formation microorganisms on solid surfaces. While most research oriented to understand bacterial surface colonization, such observations at level using photosynthetic microalgae are thus far elusive....
Microalgae play an essential role in agriculture as bio-fertilizers and biostimulants, but some also produce various plant hormones, such cytokinins (CK). CK are a class of natural hormones there is evidence that exogenous or bacterial can modulate defense. However, lack knowledge about the effect specific microalgae microalgae-produced on this response. In our study, we have evaluated cytokinins-producing Chlamydomonas reinhardtii (Cri) its ability to prime Nicotiana tabacum defenses...
Abstract Calredoxin (CRX) is a calcium (Ca 2+ )-dependent thioredoxin (TRX) in the chloroplast of Chlamydomonas reinhardtii with largely unclear physiological role. We elucidated CRX functionality by performing in-depth quantitative proteomics wild type cells comparison crx insertional mutant (IM ), two CRISPR/Cas9 KO mutants and rescues. These analyses revealed that NADPH-dependent TRX reductase (NTRC) co-regulated CRX. Electron transfer measurements inhibits reduction oxidized 2-Cys...