A5076993268- Renal Diseases and Glomerulopathies
- 3D Printing in Biomedical Research
- Renal and related cancers
- Renal cell carcinoma treatment
- Cancer Cells and Metastasis
- Genetic and Kidney Cyst Diseases
- Cellular Mechanics and Interactions
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- Chronic Kidney Disease and Diabetes
- Receptor Mechanisms and Signaling
- Birth, Development, and Health
- Immune cells in cancer
- Genetic Associations and Epidemiology
- Ion Transport and Channel Regulation
- Multiple Myeloma Research and Treatments
- MRI in cancer diagnosis
- Neonatal Respiratory Health Research
- Inflammasome and immune disorders
- Protein Degradation and Inhibitors
- Glycosylation and Glycoproteins Research
- Single-cell and spatial transcriptomics
- Diabetes Treatment and Management
- Nuclear Structure and Function
- Ubiquitin and proteasome pathways
Universitätsklinikum Erlangen
2021-2025
Friedrich-Alexander-Universität Erlangen-Nürnberg
2021-2024
RELX Group (United States)
2023
Abstract Biofabrication techniques enable the performance of bioinspired three‐dimensional (3D) matrices resembling primary tumors. To validate their reliability, embedded cells may express complex biophysical responses. Among others, emergence tumor heterogeneity and generation Polyploid Giant Cancer Cells (PGCC), as a result mechanical stress, are two most challenging hallmarks to resemble in vitro. Here, these phenomena studied cultured on two‐dimensional (2D) flasks, 3D spheroids, or...
Evolution of clear cell renal carcinoma is guided by dysregulation hypoxia-inducible transcription factor (HIF) pathways following loss the von Hippel-Lindau tumor suppressor protein. Renal (RCC)-associated polymorphisms influence HIF-DNA interactions at enhancers important oncogenes thereby modulating risk developing cancer. A strong signal genome-wide association with RCC was determined for single nucleotide polymorphism (SNP) rs4903064, located on chr14q.24.2 within an intron DPF3,...
Individuals of African ancestry carrying two pathogenic variants apolipoprotein 1 (APOL1) have a substantially increased risk for developing chronic kidney disease. The course APOL1 nephropathy is extremely heterogeneous and shaped by systemic factors such as response to interferon. However, additional environmental operating in this second-hit model been less well defined. Here, we reveal that stabilization hypoxia-inducible transcription (HIF) hypoxia or HIF prolyl hydroxylase inhibitors...
Signaling-pathway analyses and the investigation of gene responses to different stimuli are usually performed in 2D monocultures. However, within glomerulus, cells grow 3D involved direct paracrine interactions with glomerular cell types. Thus, results from monoculture experiments must be taken caution. We cultured endothelial cells, podocytes mesangial 2D/3D monocultures co-cultures analyzed survival, self-assembly, expression, cell-cell interaction, pathways using live/dead assay,...
Podocytes are critical components of the glomerular filtration barrier, sitting on outside basement membrane. Primary and secondary foot processes characteristic for podocytes, but cell that develop in culture were not studied much past. Moreover, protocols diverse visualization methods mostly can only be used one technique, due to differences fixation, drying handling. However, we detected by single-cell RNA sequencing (scRNAseq) analysis cells reveal high variability genes involved...
Objective We have recently shown that priming of synovial fibroblasts (SFs) drives arthritis flares. Pathogenic SFs is essentially mediated by epigenetic reprogramming. Bromodomain and extraterminal motif (BET) proteins translate changes into transcription. Here, we used a BET inhibitor (I‐BET151) to target inflammatory tissue reduce flare severity in murine experimental model. Methods BALB/c mice were treated intraperitoneal injection or local the paw with I‐BET151, which blocks interaction...
Abstract In this work, we studied the generation and rising of polyploid cancer cells as a product mechanical stress. To purpose, MCF7 breast were cultured on 2D ( i.e . flasks, or flat hydrogels), in 3D milieus Spheroids, immobilized within alginate-gelatin microbeads, named work tumor-like microcapsules), further analyzed by biophysical genetic methods (i.e. single-cell Traction Force Microscopy RNA-seq respectively). Our results show that preconditioned onto surfaces exhibit low number...
Mutations in polycystin-1 which is encoded by the PKD1 gene are main causes for development of autosomal dominant polycystic kidney disease. However, only little known about physiological function and even less regulation its expression. Here, we show that expression induced hypoxia compounds stabilize hypoxia-inducible transcription factor (HIF) 1 α primary human tubular epithelial cells. Knockdown HIF subunits confirms HIF-1α-dependent Furthermore, ChIP-seq reveals interacts with a...
The interplay between genetic and environmental factors influences the course of chronic kidney disease (CKD). In this context, alterations in gene MUC1 (Mucin1) predispose to development CKD. These variations comprise polymorphism rs4072037, which alters splicing mRNA, length a region with variable number tandem repeats (VNTR), rare autosomal-dominant inherited dominant-negative mutations or 5' VNTR that causes autosomal dominant tubulointerstitial (ADTKD-MUC1). As hypoxia plays pivotal...
Abstract Background Genome-wide association studies (GWAS) have identified hundreds of genetic loci associated with kidney function. By combining these findings post-GWAS information (e.g., statistical fine-mapping to identify independent signals and narrow down causal variants; or different sources annotation data), new hypotheses regarding physiology disease aetiology can be obtained. These need tested in laboratory experiments, for example, therapeutic targets. For this purpose, the...
Uncovering the function of understudied G protein-coupled receptors (GPCRs) provides a wealth untapped therapeutic potential. The poorly understood adhesion GPCR Gpr126 (Adgrg6) is widely expressed in developing kidneys. In adulthood, expression enriched parietal epithelial cells (PECs) and collecting duct urothelium. Whether plays role kidney disease remains unclear. Here, we characterized diseased kidneys mice, rats, humans. RT-PCR data show that altered disease. A quantitative RNAscope®...
Abstract Background and Aims Podocytes typically represent a distinct morphology with long, interdigitating primary secondary foot processes wrapping around glomerular capillaries forming the filtration unit of kidney. In diseases like genetic focal segmental glomerulosclerosis (FSGS), podocyte alterations are often related to dysfunction barrier development proteinuria. Genetic mutations in relevant genes, important for process or actin cytoskeleton dynamics, known induce severe injury....
Universitatsklinikum Erlangen Medizinische Klinik 4 Nephrologie und Hypertensiologie, Erlangen, Germany.