Development of new therapeutic strategies for breast cancer is urgently needed due to the sustained emergence drug resistance, tumor recurrence and metastasis. To gain a novel insight into approaches fight against cancer, cytocidal effects hellebrigenin (Helle) arenobufagin (Areno) were investigated in human estrogen receptor (ER)-positive cell line MCF-7 triple-negative MDA-MB-231. Helle exhibited more potent cytotoxicity than Areno both cells, cells susceptible drugs comparison with...

This study evaluated the educational impact of dental seminars involving practicing pharmacists and dentists on pharmacy students. Its primary objective was to assess changes in learning attitudes improvements oral care knowledge through lectures group discussions. The were conducted during October-November 2024, participants included 14 second-year Participants completed surveys before after that addressed topics such as "antibiotics care" "pharmacist-dentist collaboration." Survey results...

Chemo-sensitivity of two-dimensional (2D) monolayers and three-dimensional (3D) spheroids human breast cancer MCF-7 cells were investigated.MCF-7 cultured in or established using a thermo-reversible gelatin polymer, the presence daunorubicin, docetaxel, As2S2 Cell proliferation was examined by Counting Kit-8 assay.Daunorubicin, dose-dependently decreased cell both 2D- 3D-culture systems. The 3D less sensitive to these agents than 2D cells. Verapamil, an inhibitor P-glycoprotein, partially...

The need for novel therapeutic strategies breast cancer remains paramount given the sustained development of drug resistance, tumor recurrence and metastasis. Trivalent arsenic derivatives (arsenite, AsIII) with remarkable clinical efficacy in acute promyelocytic leukemia has been demonstrated to exhibit inhibitory effect against cells. We recently that AsIII plus tetrandrine (Tetra), a Chinese plant-derived alkaloid, exerted potent antitumor activity human cells, however, underlying...

Oral mucositis (OM) is a common side effect in patients with cancer receiving chemotherapy and radiotherapy; however, no salivary mediator known to be associated OM. We aimed determine candidate inflammatory mediators potentially OM cancer. To this end, we compared the relationships between grade, oral mucosal dryness, (Interleukin (IL)-1β, IL-6, IL-10, IL-12p70, tumor necrosis factor (TNF), prostaglandin E2, vascular endothelial growth factor) healthy volunteers (HV). collected saliva...

Several cytokines secreted from breast cancer tissues are suggested to be related disease prognosis. We examined Th1/Th2/Th17 produced three-dimensionally cultured and them with patient clinical profiles.21 tumor 9 normal surgically resected patients were in thermoreversible gelatin polymer-containing medium. Tissue growth cytokine concentrations the culture medium analyzed hormone receptor expressions profiles.IL-6 IL-10 expressed highly of both tissues. However, IFN-γ, TNF-α, IL-2, IL-17A...

A major component of the extracellular matrix (ECM), laminins, modulates cells via diverse receptors. Their fragments have emerging utility as components "ECM-mimetics" optimized to promote cell-based therapies. Recently, we reported that a bioactive laminin peptide known A99 enhanced cell binding and spreading fusion an elastin-like polypeptide (ELP). The ELP "handle" serves rapid, noncovalent strategy concentrate mixtures onto surface. We now report this can be further generalized across...

Fasudil and ozagrel are drugs with the same indications for treatment of cerebral vasospasm in Japan. However, there have been no definitive conclusions on clinical efficacy fasudil hydrochloride sodium monotherapy or their combination. Therefore, we aimed to investigate effectiveness combined administration Japanese patients subarachnoid hemorrhage (SAH).

Some aspects regarding the use of antiplatelet agents after coil embolization for subarachnoid hemorrhage during admission remain unclear. This study used diagnostic procedure combination (DPC) data to investigate safety and prognostic effects aspirin monotherapy P2Y12 inhibitor therapy on bleeding events. cross-sectional Japanese DPC assess patients who were hospitalized with received between April 2016 March 2020 (n = 4421). The (A group, n 2848) (AP 1573) groups compared. primary...

In China, arsenic disulfide (As2S2) has been used for the treatment of hematological malignancies. The present study aimed to evaluate effects As2S2 on human breast cancer MCF‑7 cell line cultured in both two‑dimensional (2D) monolayers and three‑dimensional (3D) spheroids explore its therapeutic potential treatment. Cellular viability induction apoptosis were examined with a counting kit‑8 (CCK‑8) assay flow cytometric analysis, respectively. Alterations expression levels...

Three-dimensionally (3D) cultured tumor cells (spheroids) exhibit more resistance to therapeutic agents than the in traditional two-dimensional (2D) system (monolayers). We previously demonstrated that arsenic disulfide (As 2 [Formula: see text] exerted significant anticancer efficacies both 2D- and 3D-cultured MCF-7 cells, whereas 3D spheroids were shown be resistant As S treatment. L-buthionine-(S, R)-sulfoximine (BSO), an inhibitor of glutathione (GSH) synthesis, has been regarded a...

Proteinuria is a major side-effect of the anti-tumor drug bevacizumab, although its incidence and risk factors in real world are still unclear. Although renin-angiotensin-aldosterone system inhibitors used clinically to prevent proteinuria, their efficacy remains The aim present study was reveal bevacizumab-induced proteinuria examine effectiveness antihypertensive drugs preventing proteinuria. We conducted retrospective cohort using National Hospital Organization Clinical Data Archives...

Hochuekkito (HET), Juzentaihoto (JTT), and Ninjin’yoeito (NYT) have been used as Hozai, a group of traditional Japanese herbal medicines, to treat physically mentally weak cancer patients. Their compositions are quite different, pharmaceutical companies using different types or quantities herbs for formulations with the same name. Here, we compared immunological differences between HET, JTT, NYT respect induced T cell subsets cytokines. Peripheral blood mononuclear cells (PBMCs) were...

Cepharanthine (CEP) is a bis-bynzelisoquinoline alkaloid from the same class as anticancer agent tetrandrine (TET). However, effects of CEP against breast cancer have not been extensively studied, despite its long therapeutic history with low toxicity other types cancer. 3D culture systems more accurately mimic human body and address limitations determining drug effectiveness compared 2D systems. In present study, antitumor activities TET were in triple-negative (TNBC) MDA-MB-231 estrogen...

Approximately a decade has passed since the addition of venous thromboembolism to list significant adverse reactions antipsychotic drugs. However, only few studies have investigated relationship between use and in Japanese population. We aimed evaluate risk recurrent users drugs update evidence on A cross-sectional retrospective analysis data from large claims database, managed by Medical Data Vision Co. Ltd., was conducted. Adult patients who experienced October 2014 September 2018 acute...

Aromatase inhibitor use for postmenopausal hormone-sensitive breast cancer patients often results in drug-induced osteoarthropathy, while its accurate mechanism has not been clarified. We investigated the implication of female hormones and several cytokines osteoarthropathy complicated with aromatase treatment, efficacy vitamin E on severity patients.Sixty two treated average 1.77 years were included. These orally administered (150mg/day) 29.8 days to alleviate inhibitor-related...

The measurement of the uniformity preparations is an important component quality assessment. This study was conducted to evaluate appropriate mixing time for external using a planetary centrifugal mixer. Steroid ointment and white petrolatum (5 g each) were added 13-mL container mixed mixer (NRE-120®, THINKY) at rotation speed 800 rpm revolution 2000 rpm. We sampled 0.1 mixture from three spots (the top, middle, bottom portions) container, measured steroid content, evaluated product mixtures...

Abstract Objectives Managing perioperative anxiety improves postoperative recovery significantly by reducing pain. However, little is known about association between preoperative and pain in oral surgical procedures. In the present study, we examined effect mandibular third molar extraction anxiolytic of Yokukansan (YKS) during period. Materials methods We conducted a prospective cohort study from September 2021 to May 2022. For patients with complaint insomnia on day before surgery, 2.5 g...

When mixing and compounding injectable agents, performing aseptic manipulations on a clean bench maintained according to the requirements of US Federal Standard Class 100 (≤100 pcs/ft3) is crucial for prevention contamination by airborne particulates or microbes. Owing air turbulence generated other factors, level cleanliness in may be affected configuration environment. We configured benches different environments with mean particulate counts (A) 1,000, (B) 30,000, (C) 300,000/ft3 sash...

Poor medication compliance in children can affect treatment efficacy. We examined the impact of a picture book created by pharmacists to improve children. Our study aim was assess effects pharmacist-created on and their parents collaboration with an outpatient pharmacy. This included 74 [recovery rate 28/74 (37.8%)] aged 3-6 years between March 2023 2024. In item "Have you experienced any difficulties giving your child?" proportion respondents answering, "Always" or "Sometimes" decreased...

Prednisolone (PSL), a type of corticosteroid used to treat autoimmune diseases, can increase the risk infection and osteoporosis. Saireito (114), Kampo medicine, has an immunosuppressive effect; with its use, dose steroids be reduced. However, mechanism when PSL is still unclear. We peripheral blood mononuclear cells (PBMCs) from healthy adults examine effect 114 treatment on PBMC proliferation, T-cell subsets, cytokine production. PBMCs were cotreated concanavalin A 300 μM (either Tsumura &...