A5034777795- Angiogenesis and VEGF in Cancer
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Orthopaedic implants and arthroplasty
- Cardiovascular Disease and Adiposity
- Dental Implant Techniques and Outcomes
- AI in cancer detection
- Cancer, Hypoxia, and Metabolism
- Multiple Myeloma Research and Treatments
- Additive Manufacturing and 3D Printing Technologies
- Head and Neck Cancer Studies
- Algal biology and biofuel production
- Cellular Mechanics and Interactions
- CAR-T cell therapy research
- Mathematical Biology Tumor Growth
- Lung Cancer Diagnosis and Treatment
- ATP Synthase and ATPases Research
- Protein Degradation and Inhibitors
- Autophagy in Disease and Therapy
- Immunotherapy and Immune Responses
- Adipokines, Inflammation, and Metabolic Diseases
- Monoclonal and Polyclonal Antibodies Research
King's College London
2019-2024
Guy's Hospital
2020-2023
Cell Therapy Catapult
2022
San Raffaele University of Rome
2011-2014
VIB-KU Leuven Center for Cancer Biology
2012-2013
KU Leuven
2012-2013
Vita-Salute San Raffaele University
2009
Istituti di Ricovero e Cura a Carattere Scientifico
2009
Mario Negri Institute for Pharmacological Research
2004
The purpose of this study was to test the hypothesis that specific epicardial adipose tissue (EAT) proinflammatory adipokines might be implicated in acute coronary syndrome (ACS). We compared expression and protein secretion several EAT male ACS with those matched stable artery disease (CAD) patients controls angiographically normal arteries. effect supernatant cultured on endothelial cell permeability vitro also evaluated three groups. showed significantly higher gene resistin than CAD....
Osteochondral defects remain a major clinical challenge mainly due to the combined damage articular cartilage and underlying bone, interface between two tissues having very different properties. Current treatment modalities have several limitations drawbacks, with limited capacity of restoration; however, tissue engineering shows promise in improving outcomes osteochondral defects. In this study, novel gradient scaffold has been fabricated, implementing structure design mimic anatomical,...
A growing body of evidence suggests that chromogranin (CgA), a secretory protein released by many neuroendocrine cells and frequently used as diagnostic prognostic serum marker for range tumors, is precursor several bioactive fragments. This work was undertaken to assess whether the N-terminal fragment CgA(1-76) (called vasostatin I) can inhibit proangiogenic activity vascular endothelial growth factor (VEGF), involved in tumor growth. The effect recombinant human I (VS-1) on VEGF-induced...
To compare mechanical properties of 3D-printed and milled poly-ether-ether-ketone (PEEK) materials. define post-production treatments to enhance biocompatibility PEEK.Standardised PEEK samples were produced via milling fused-deposition-modelling 3D-printing. evaluate properties, tensile strength, maximum flexural fracture toughness, micro-hardness measured.3D printed sandblasted with 50 or 125 μm aluminium oxide beads increase biocompatibility.Scanning electron microscopy (SEM) evaluated...
We present a new continuously perfusable, customisable, and matrix free vasculature on chip (VoC) platform enabling to study microvascular functions responses perturbations under physiologic perfusive flow.
ABSTRACT α‐Synuclein (α‐syn) is a 140‐amino acid presinaptic protein whose mutations A30P and A53T have been linked to familiar Parkinson’s disease (PD). Many data suggest that α‐syn aggregation the key event triggers α‐syn‐mediated neurotoxicity. Nevertheless, other lines of evidence proposed protective role against oxidative stress (a major feature PD), even if exact mechanism this action pathogenetic respect not elucidated yet. To address these points, we developed an in vitro model by...
Tumour-associated angiogenesis plays a key role at all stages of cancer development and progression by providing nutrient supply, promoting the creation protective niches for therapy-resistant stem cells, supporting metastatic cascade. Therapeutic strategies aimed vascular targeting, including vessel disruption and/or normalization, have yielded promising but inconsistent results, pointing to need set up reliable models dissecting steps angiogenic process, as well ways interfere with them,...
The angiogenic switch is a fundamental process for many diseases and tumor growth. main proangiogenic stimulus hypoxia, through activation of the hypoxia-inducible factor (HIF)-1α pathway in endothelial cells (ECs). We have previously shown that vasostatin-1 (VS-1) fragment chromogranin A inhibits TNF-α-induced vessel permeability VEGF-induced EC proliferation, together with migration matrix invasion, which are all critical steps angiogenesis. present study was undertaken to investigate...
Routine haematoxylin and eosin (H&E) photomicrographs from human papillomavirus-associated oropharyngeal squamous cell carcinomas (HPV + OpSCC) contain a wealth of prognostic information. In this study, we developed high content image analysis (HCIA) workflow to quantify features H&E images HPV OpSCC patients identify predict patient outcomes.First, have an open-source HCIA tool for single-cell segmentation classification images. Subsequently, used our analyse set 889 diagnostic slides in...
Endothelial cells (ECs) are heterogeneous across and within tissues, reflecting distinct, specialised functions. EC heterogeneity has been proposed to underpin plasticity independently from vessel microenvironments. However, driven by contact-dependent or short-range cell-cell crosstalk cannot be evaluated with single cell transcriptomic approaches, as spatial contextual information is lost. Nonetheless, quantification of understanding its molecular drivers key developing novel therapeutics...
Dental rehabilitation post-radiotherapy often requires the consideration of dental implants. However, these are tentatively prescribed due to concern hypovascularisation and possible osteoradionecrosis. Hence, current study assessed microvasculature dento-alveolar bone at implant sites taking into exact radiotherapy dose received region.Bone cores were taken from nine patients during treatment compared control patients. Specimens stained using CD31 digitalised a high-resolution scanner for...
Endothelial cells (ECs) are widely heterogeneous at the cell level and serve different functions vessel tissue levels. EC-forming colonies derived from induced pluripotent stem (iPSC-ECFCs) alongside models such as primary human umbilical vein ECs (HUVECs) slowly becoming available for research with future applications in therapies, disease modeling, drug discovery. We others previously described high-content analysis approaches capturing unbiased morphology-based measurements coupled...
Biofunctionalization was investigated for polymers and metals considering their scarce integration ability. On the contrary few studies dealt with ceramic biofunctionalization because bioactive bioresorbable surfaces of ceramics are able to positively interact biological environment. In this study cell-response improvement on biofunctionalized wollastonite diopside-based scaffolds demonstrated. The were first obtained by heat treatment a silicone embedding reactive oxide fillers then...
Bone is a highly vascularized tissue and relies on the angiogenesis response of cells in immediate environmental niche at defect site for regeneration. Hence, ability to control cellular responses during osteogenesis has important implications tissue-engineered strategies. Self-assembling ionic-complementary peptides have received much interest as they mimic natural extracellular matrix. Three-dimensional (3D)-printed biphasic calcium phosphate (BCP) scaffolds coated with self-assembling DAR...
Abstract Endothelial cells (EC) are heterogeneous across and within tissues, reflecting distinct, specialised functions. EC heterogeneity has been proposed to underpin plasticity independently from vessel microenvironments. However, driven by contact-dependent or short-range cell-cell crosstalk cannot be evaluated with single cell transcriptomic approaches as spatial contextual information is lost. Nonetheless, quantification of understanding its molecular drivers key developing novel...
Abstract Creating vascularised cellular environments in vitro is a current challenge tissue engineering and bottleneck towards developing functional stem cell-derived microtissues for regenerative medicine basic investigations. Here we have developed new workflow to manufacture Vasculature on Chip (VoC) systems efficiently, quickly, inexpensively. We employed 3D printing fast-prototyping of bespoke VoC coupled them with refined organotypic culture system (OVAA) grow patent capillaries using...