A5016150476- Malaria Research and Control
- RNA and protein synthesis mechanisms
- Gene expression and cancer classification
- Polyamine Metabolism and Applications
- Genomics and Phylogenetic Studies
- Genetic Associations and Epidemiology
- Trypanosoma species research and implications
- Mosquito-borne diseases and control
- Insect Resistance and Genetics
- Genetic and phenotypic traits in livestock
- Genetic diversity and population structure
- Computational Drug Discovery Methods
- HIV/AIDS drug development and treatment
- Bioinformatics and Genomic Networks
- RNA Research and Splicing
- Synthesis and Biological Evaluation
- Research on Leishmaniasis Studies
- Monoclonal and Polyclonal Antibodies Research
- Evolution and Genetic Dynamics
- Genetic Mapping and Diversity in Plants and Animals
- demographic modeling and climate adaptation
- Genomics and Chromatin Dynamics
- Synthesis and biological activity
- Developmental Biology and Gene Regulation
- Livestock Farming and Management
National Science and Technology Development Agency
2014-2024
National Center for Genetic Engineering and Biotechnology
2014-2024
Aarhus Business College
2017
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2015
Département Génétique Animale
2015
Institut de Recherche pour le Développement
2015
Centre de Biologie et de Gestion des Populations
2015
Khon Kaen University
2015
Mahidol University
2015
United Utilities (United Kingdom)
2009
The spread of high-level pyrimethamine resistance in Africa threatens to curtail the therapeutic lifetime antifolate antimalarials. We studied possible evolutionary pathways evolution using an approach which all mutational intermediates were created by site-directed mutagenesis and assayed for their level drug resistance. coding sequence dihydrofolate reductase (DHFR) from malaria parasite Plasmodium falciparum was mutagenized, tests carried out Escherichia coli under conditions endogenous...
Conventional reverse genetic approaches for study of Plasmodium malaria parasite gene function are limited, or not applicable. Hence, new inducible systems needed. Here we describe a method to control P. falciparum expression in which target genes bearing glmS ribozyme the 3' untranslated region efficiently knocked down transgenic parasites response glucosamine inducer. Using reporter genes, show that cleaves mRNA vivo leading reduction following treatment. Glucosamine-induced activation led...
The malaria parasite Plasmodium falciparum exports several hundred proteins into the infected erythrocyte that are involved in cellular remodeling and severe virulence. export mechanism involves element (PEXEL), which is a cleavage site for protease, Plasmepsin V (PMV). PMV gene refractory to deletion, suggesting it essential, but definitive proof lacking. Here, we generated PEXEL-mimetic inhibitor potently blocks activity of isolated from P. vivax. Assessment revealed PEXEL occurs...
SUMMARY Interacting genetic elements need to coevolve if their joint function is be maintained; for example, the correct binding of transcriptional regulators defined sites in gene promoters needs maintained during evolution ensure proper function. As part a wider investigation into molecular coevolution Dipteran homeodomain‐bearing regulator bicoid ( bcd ) and Bcd‐dependent promoters, we present data on functional, structural, sequence differences between segmentation hunchback hb ),...
SUMMARY To what extent and in way do gene promoters their transacting regulatory proteins coevolve? In this earlier publications we show that the Bicoid‐dependent of segmentation genes hunchback tailless species higher Diptera ( Drosophila , Musca Calliphora Lucilia ) are different with respect to copy number, spacing, sequence, orientation Bicoid binding sites. At same time there significant amino acid differences homeodomain. test these interspecific differences, used a series functional...
The HUGO Pan-Asian SNP consortium conducted the largest survey to date of human genetic diversity among Asians by sampling 1,719 unrelated individuals 71 populations from China, India, Indonesia, Japan, Malaysia, Philippines, Singapore, South Korea, Taiwan, and Thailand. We have constructed a database (PanSNPdb), which contains these data various new analyses them. PanSNPdb is research resource in analysis population structure Asian peoples, including linkage disequilibrium patterns,...
Abstract Background Genome-wide association studies (GWAS) do not provide a full account of the heritability genetic diseases since gene-gene interactions, also known as epistasis are considered in single locus GWAS. To address this problem, considerable number methods have been developed for identifying disease-associated interactions. However, these typically fail to identify interacting markers explaining more disease over GWAS, many interactions significant obscured by uninformative...
To maximize the potential of genomics in medicine, it is essential to establish databases genomic variants for ethno-geographic groups that can be used filtering and prioritizing candidate pathogenic variants. Populations with non-European ancestry are poorly represented among current variant databases. Here, we report first high-density survey Thai population, Reference Exome (T-REx) database. T-REx comprises exome sequencing data 1092 unrelated individuals. The targeted regions common four...
Control of malaria is threatened by emerging parasite resistance to artemisinin and derivative drug (ART) therapies. The molecular detail how Plasmodium parasites respond ART this could contribute are not well understood. To address question, we performed a transcriptomic study dihydroartemisinin (DHA) response in P. falciparum K1 strain berghei ANKA using microarray RNA-seq technology. Microarray data from DHA-treated trophozoite stage revealed pattern that overall less trophozoite-like...
There is considerable ethno-linguistic and genetic variation among human populations in Asia, although tracing the origins of this diversity complicated by migration events. Thailand at center Mainland Southeast Asia (MSEA), a region within that has not been extensively studied. Genetic substructure may exist Thai population, since waves from southern China throughout its recent history have contributed to substantial gene flow. Autosomal SNP data were collated for 438,503 markers 992...
The S108N mutation of dihydrofolate reductase (DHFR) renders Plasmodium falciparum malaria parasites resistant to pyrimethamine through steric clash with the rigid side chain inhibitor. Inhibitors flexible chains can avoid this and retain effectiveness against mutant. However, other mutations such as N108S reversion confer resistance inhibitors. We designed synthesized hybrid inhibitors two structural types in a single molecule, which are effective both wild-type multiple mutants P. their...
Extensive sequence analysis of the developmental gene hunchback and its 5' 3' regulatory regions in Drosophila melanogaster, virilis, Musca domestica, Tribolium castaneum, using a variety computer algorithms, reveals high simplicity probably generated by slippage-like mechanisms turnover. No are entirely refractory to action slippage, although density composition simple motifs varies from region region. Interestingly, flanking share short repetitive despite their separation itself, different...
SUMMARY The homeobox gene bicoid functions as an anterior pattern organizer of the Drosophila embryo, but other than in higher flies (Cyclorrhapha), orthologues appear to be absent from insect genomes. In , is expressed anterior‐to‐posterior protein gradient and regulates spatially restricted expression domains segmentation genes a concentration‐dependent manner. hunchback direct transcriptional target Bicoid, complements “morphogen” activity Bicoid. activated by Bicoid throughout half...
Abstract Background Non-random patterns of genetic variation exist among individuals in a population owing to variety evolutionary factors. Therefore, populations are structured into genetically distinct subpopulations. As genotypic datasets become ever larger, it is increasingly difficult correctly estimate the number subpopulations and assign them. The computationally efficient non-parametric, chiefly Principal Components Analysis (PCA)-based methods thus becoming relied upon for structure...
Abstract Background The ever increasing sizes of population genetic datasets pose great challenges for structure analysis. Tracy-Widom (TW) statistical test is widely used detecting structure. However, it has not been adequately investigated whether the TW statistic susceptible to type I error, especially in large, complex datasets. Non-parametric, Principal Component Analysis (PCA) based methods resolving have developed which rely on test. Although PCA-based can resolve structure, they...
Cattle commonly raised in Thailand have characteristics of Bos indicus (zebu). We do not know when or how cattle domestication occurred, and so questions remain regarding their origins relationships to other breeds. obtained genome-wide SNP genotypic data 28 bovine individuals sampled from four regions: North (Kho-Khaolampoon), Northeast (Kho-Isaan), Central (Kho-Lan) South (Kho-Chon) Thailand. These regional varieties distinctive traits suggestive breed-like genetic variations. From these...
Sugarcane is an important global food crop and energy resource. To facilitate the sugarcane improvement program, genome gene information are for studying traits at molecular level. Most currently available transcriptome data were generated using second-generation sequencing platforms, which provide short reads. The de novo assembled transcripts from these limited in length, hence may be incomplete inaccurate, especially long RNAs.We a dataset of leaf tissue commercial Thai cultivar Khon Kaen...
SUMMARY To further understand the evolutionary dynamics of regulatory interactions underlying development, we expand on our previous analysis hunchback and compare structure function tailless enhancer between Musca domestica Drosophila melanogaster . Our shows that although expression patterns functional protein domains tll are conserved , sequences unalignable. Upon closer investigation, find these highly diverged encode same information necessary for Bicoid, Dorsal, terminal system to...