A5015413405- Plant Molecular Biology Research
- Mass Spectrometry Techniques and Applications
- Plant nutrient uptake and metabolism
- Analytical Chemistry and Chromatography
- Ion-surface interactions and analysis
- Advanced Proteomics Techniques and Applications
- RNA and protein synthesis mechanisms
- Plant Gene Expression Analysis
- RNA modifications and cancer
- Plant Stress Responses and Tolerance
- Molecular Biology Techniques and Applications
- Plant responses to water stress
- Legume Nitrogen Fixing Symbiosis
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Photosynthetic Processes and Mechanisms
- Research in Cotton Cultivation
- Ferroelectric and Piezoelectric Materials
- Multiferroics and related materials
- Environmental DNA in Biodiversity Studies
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Plant Reproductive Biology
- Plant tissue culture and regeneration
Chinese Academy of Sciences
2013-2025
Lanzhou University
2025
First Hospital of Lanzhou University
2025
University of Chinese Academy of Sciences
2023-2025
Shanghai Institute of Ceramics
2023-2025
Purdue University West Lafayette
2014-2024
Nanyang Technological University
2006-2024
Nanjing University of Aeronautics and Astronautics
2023-2024
China Geological Survey
2024
NARI Group (China)
2024
Significance DNA methylation is generally considered an epigenetic mark for transcriptional gene silencing. In this work, we generated loss-of-function mutant alleles of SlDML2 . We characterized the fruits that failed to ripen and discovered required demethylation activation genes important fruit ripening, including involved in pigment flavor synthesis, ethylene synthesis signaling, cell wall hydrolysis. Unexpectedly, found SlDML2-mediated also necessary ripening-induced repression hundreds...
Active DNA demethylation is an important part of epigenetic regulation in plants and animals. How active regulated its relationship with histone modification patterns are unclear. Here, we report the discovery IDM1, a regulator Arabidopsis. IDM1 required for preventing hypermethylation highly homologous multicopy genes other repetitive sequences that normally targeted by Repressor Silencing 1 related 5-methylcytosine glycosylases. binds methylated at chromatin sites lacking H3K4 di- or...
Significance DNA methylation is critical for transposon silencing and gene regulation. levels are determined by the combined activities of methyltransferases demethylases. This study found a 39-bp monitoring sequence (MEMS) in promoter demethylase REPRESSOR OF SILENCING 1 ( ROS1 ) Arabidopsis plants. MEMS responsive to both RNA-directed ROS1-dependent active demethylation. Thus, can sense demethylation activities, it regulates genomic adjusting expression levels. Our results suggest that...
DNA methylation is an important epigenetic mark involved in many biological processes. The genome of the climacteric tomato fruit undergoes a global loss due to active demethylation during ripening process. It unclear whether other fruits also associated with demethylation. We characterized single-base resolution methylomes sweet orange fruits. Compared immature fruits, ripe gained at over 30,000 genomic regions and lost about 1,000 regions, suggesting increase ripening. This was correlated...
Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic amplified PCR, and the products covalently attached to chip wells via N-succinimidyl (4-iodoacetyl)aminobenzoate (SIAB) chemistry. Primer annealing, extension, termination performed on a 1-microl scale directly in parallel. Diagnostic thus generated detected situ using matrix-assisted laser desorption ionization This miniaturized method has potential accurate, high-throughput, low-cost...
DNA methylation is an important epigenetic mark in many eukaryotic organisms. De novo plants can be achieved by the RNA-directed (RdDM) pathway, where plant-specific DNA-dependent RNA polymerase IV (Pol IV) transcribes target sequences to initiate 24-nt siRNA production and action. The putative binding protein DTF1/SHH1 of Arabidopsis has been shown associate with Pol required for accumulation transcriptional silencing at several RdDM loci. However, extent mechanism DTF1 function unclear. We...
m6A is a ubiquitous RNA modification in eukaryotes. Transcriptome-wide patterns Arabidopsis have been assayed recently. However, differential between organs not well characterized. Over two-third of the transcripts are modified by m6A. In contrast to recent observation enrichment 5′ mRNA, we find that distributed predominantly near stop codons. Interestingly, 85 % show high methylation extent compared their transcript level. The 290 highly methylated mainly associated with transporters,...
A recent paper by Kidokoro et al. (2020) in The Plant Cell reported a transgene-dependent transcriptional silencing phenomenon the dominant ice1-1 Arabidopsis mutant containing CBF3-LUC reporter, and questioned whether ICE1 may regulate CBF genes be involved plant cold response. Here, we evaluate available evidence supporting involvement of response, provide ChIP-seq data showing binding to promoters other regulatory known critical for response as well some COR genes.
Transposons are generally kept silent by epigenetic mechanisms including DNA methylation. Here, we identified a pair of H arbinger transposon-derived proteins (HDPs), HDP1 and HDP2, as anti-silencing factors in Arabidopsis. hdp1 hdp2 mutants displayed an enhanced silencing transgenes some transposons. Phylogenetic analyses revealed that HDP2 were co-domesticated from the Harbinger transposon-encoded transposase DNA-binding protein, respectively. interacts with nucleus, analogous to their...
Article5 December 2019Open Access Rhizobacterium-derived diacetyl modulates plant immunity in a phosphate-dependent manner Rafael JL Morcillo Shanghai Center for Plant Stress Biology, and CAS Excellence Molecular Sciences, Chinese Academy of Shanghai, China Search more papers by this author Sunil K Singh Danxia He University Beijing, Guo An Juan I Vílchez Kai Tang Department Horticulture & Landscape Architecture, Purdue University, West Lafayette, IN, USA Fengtong Yuan Yazhou Sun Chuyang...
MicroRNAs (miRNAs) regulate gene expression and play critical roles in growth development as well stress responses eukaryotes. miRNA biogenesis plants requires a processing complex that consists of the core components DICER-LIKE 1 (DCL1), SERRATE (SE) HYPONASTIC LEAVES (HYL1). Here we show inactivation functionally redundant members SnRK2 kinases, which are abscisic acid (ABA) osmotic signaling pathways, leads to reduction accumulation under conditions. Further analysis revealed steady state...
Several nucleoporins in the nuclear pore complex (NPC) have been reported to be involved abiotic stress responses plants. However, molecular mechanism of how NPC regulates responses, especially expression responsive genes remains poorly understood. From a forward genetics screen using an stress-responsive luciferase reporter (RD29A-LUC) sickle-1 (sic-1) mutant background, we identified suppressor caused by mutation NUCLEOPORIN 85 (NUP85), which exhibited reduced RD29A-LUC response ABA and...
Salt stress is a major environmental factor limiting plant growth and productivity. We recently discovered an important new salt tolerance pathway, where the cell wall leucine-rich repeat extensins LRX3/4/5, RAPID ALKALINIZATION FACTOR (RALF) peptides RALF22/23 receptor-like kinase FERONIA (FER) function as module to simultaneously regulate tolerance. However, intracellular signaling pathways that are regulated by extracellular LRX3/4/5-RALF22/23-FER coordinate growth, integrity responses...
Active DNA demethylation is critical for controlling the methylomes in plants and mammals. However, little known about how demethylases are recruited to target loci, involvement of chromatin marks this process. Here, we identify 2 components SWR1 chromatin-remodeling complex, PIE1 ARP6, as required ROS1-mediated demethylation, discover SWR1-associated bromodomain-containing proteins, AtMBD9 nuclear protein X1 (NPX1). NPX1 recognize histone acetylation established by increased methylation 1...
CDK8 is a key subunit of Mediator complex, large multiprotein complex that fundamental part the conserved eukaryotic transcriptional machinery. However, biological functions in plant abiotic stress responses remain largely unexplored. Here, we demonstrated as critical regulator abscisic acid (ABA) signaling and drought response pathways Arabidopsis. Compared to wild-type, cdk8 mutants showed reduced sensitivity ABA, impaired stomatal apertures hypersensitivity stress. Transcriptomic...
Solar-blind UV photodetectors have outstanding reliability and sensitivity in flame detection without interference from other signals response quickly. Herein, we fabricated a solar-blind photodetector based on La2O3/ε-Ga2O3 p-n heterojunction with typical type-II band alignment. Benefiting the photovoltaic effect formed by space charge region across junction interface, exhibited self-powered photocurrent of 1.4 nA at zero bias. Besides, this demonstrated excellent photo-to-dark current...
Helical polymers appended with paired structurally different enantiomers, which have opposing helical sense preferences, yield a new kind of relationship between optical activity and temperature, also reveal unusual details the nature chiral interactions. Consistent statistical physical theory developed for these experiments, proportion competing groups, determined by synthesis, fixes compensation temperature at senses are equally populated. The lyotropic liquid crystal state formed yields...