A5076109683- Asthma and respiratory diseases
- Respiratory viral infections research
- Pneumonia and Respiratory Infections
- IL-33, ST2, and ILC Pathways
- Extracellular vesicles in disease
- Pediatric health and respiratory diseases
- MicroRNA in disease regulation
- Neonatal Respiratory Health Research
- Cancer-related molecular mechanisms research
- Heavy Metal Exposure and Toxicity
- Circular RNAs in diseases
- Genetic Associations and Epidemiology
- Antibiotics Pharmacokinetics and Efficacy
- Receptor Mechanisms and Signaling
- Pharmaceutical studies and practices
- COVID-19 Clinical Research Studies
- Respiratory and Cough-Related Research
- Air Quality and Health Impacts
- Antibiotic Resistance in Bacteria
- Virus-based gene therapy research
- Trace Elements in Health
- Emergency and Acute Care Studies
- Immune Cell Function and Interaction
- Bacterial Infections and Vaccines
- Influenza Virus Research Studies
Harvard University
2013-2024
Shanghai Children's Hospital
2010-2024
Shanghai Jiao Tong University
2008-2024
ShenZhen People’s Hospital
2024
Shanghai Clinical Research Center
2021-2024
The People's Hospital of Guangxi Zhuang Autonomous Region
2024
Southern University of Science and Technology
2022-2023
Jinan University
2022-2023
Renmin University of China
2020-2023
Institute of Forest Ecology, Environment and Protection
2023
Mammalian cells are capable of delivering multiple types membrane capsules extracellularly. The limiting late endosomes can fuse with the plasma membrane, leading to extracellular release multivesicular bodies (MVBs), initially contained within endosomes, as exosomes. Budding viruses exploit TSG101 protein and endosomal sorting complex required for transport (ESCRT) machinery used MVB formation mediate egress viral particles from host cells. Here we report discovery a virus-independent...
Tracking longitudinal measurements of growth and decline in lung function patients with persistent childhood asthma may reveal links between subsequent chronic airflow obstruction.
ABSTRACT A total of 685 clinical Streptococcus pneumoniae isolates from patients with pneumococcal diseases were collected 14 centers in 11 Asian countries January 2000 to June 2001. The vitro susceptibilities the antimicrobial agents determined by broth microdilution test. Among tested, 483 (52.4%) not susceptible penicillin, 23% intermediate, and 29.4% penicillin resistant (MICs ≥ 2 mg/liter). Isolates Vietnam showed highest prevalence resistance (71.4%), followed those Korea (54.8%), Hong...
Abstract Majority of disease-modifying therapeutic targets are restricted to the intracellular space and therefore not druggable using existing biologic modalities. The ability efficiently deliver macromolecules inside target cells or tissues would greatly expand current landscape for future generations drugs, but remains challenging. Here we report use extracellular vesicles, known as arrestin domain containing protein 1 [ARRDC1]-mediated microvesicles (ARMMs), packaging delivery a myriad...
ABSTRACT Paracrine and endocrine roles have increasingly been ascribed to extracellular vesicles (EVs) generated by multicellular organisms. Central the biogenesis, content, function of EVs are their delimiting lipid bilayer membranes. To evaluate research progress on membranes EVs, International Society for Extracellular Vesicles (ISEV) conducted a workshop in March 2018 Baltimore, Maryland, USA, bringing together key opinion leaders hands‐on researchers who were selected basis submitted...
BackgroundGenetic variants in the chromosomal region 17q21 are consistently associated with asthma. However, mechanistic studies have not yet linked any of to a function that could influence asthma, and as result, identity asthma gene(s) remains elusive.ObjectivesWe sought identify characterize functional locus.MethodsWe used Exome Aggregation Consortium browser coding (amino acid–changing) locus. We obtained association measures for these both Genetic Epidemiology Research Adult Health...
Down-regulation of mitogenic signaling in mammalian cells relies part on endosomal trafficking activated receptors into lysosomes, where the are degraded. These events mediated by ubiquitination cargo and its consequent sorting multivesicular bodies that form at surfaces late endosomes. Tumor susceptibility gene 101 ( tsg101 ) recently was found to be centrally involved this process. Here we report TSG101 interacts with hepatocyte growth factor-regulated tyrosine kinase substrate (HRS), an...
Asthma is a chronic inflammatory respiratory disease that affects over 300 million people worldwide. Glucocorticoids are mainstay therapy for asthma because they exert anti-inflammatory effects in multiple lung tissues, including the airway smooth muscle (ASM). However, mechanism by which glucocorticoids suppress inflammation ASM remains poorly understood. Using RNA-Seq, high-throughput sequencing method, we characterized transcriptomic changes four primary human cell lines were treated with...
Multiple intergenic single-nucleotide polymorphisms (SNPs) near hedgehog interacting protein (HHIP) on chromosome 4q31 have been strongly associated with pulmonary function levels and moderate-to-severe chronic obstructive disease (COPD). However, whether the effects of variants in this region are related to HHIP or another gene has not proven. We confirmed genetic association SNPs COPD genome-wide study (GWAS) a Polish cohort containing severe cases healthy smoking controls (P = 0.001...
The egress of HIV particles from virus-infected cells is accomplished by the recruitment proteins that normally mediate host cell endocytic functions. This process requires interaction Gag protein with TSG101 (tumor susceptibility gene 101). Here, we report use a bacterial reverse two-hybrid system to identify cyclic peptides interfere Gag−TSG101 and finding five amino acid peptide discovered this approach can disrupt consequently inhibit egress. inhibiting molecule, which was selected...
Regulations on e-cigarettes in the U.S. do not provide guidelines chemical content of e-cigarette liquids. We evaluated emissions aldehydes and flavoring chemicals vapor under typical usage conditions. selected 24 flavors from top selling disposable brands. E-cigarettes were connected to a pump drawing air for two second puffs with sixty-second intervals between puffs. The was analyzed presence using high-performance liquid chromatography-ultraviolet detector gas chromatography an electron...
Bronchodilator response (BDR) is an important asthma phenotype that measures reversibility of airway obstruction by comparing lung function (i.e. FEV(1)) before and after the administration a short-acting β(2)-agonist, most common rescue medications used for treatment asthma. BDR also serves as test β(2)-agonist efficacy. complex trait partly under genetic control. A genome-wide association study (GWAS) BDR, quantified percent change in baseline FEV(1) was performed with 1,644 non-Hispanic...
Air pollution from particulate matter (PM) has been linked to cardiovascular morbidity and mortality; however the underlying biological mechanisms remain be uncovered. Gene regulation by microRNAs (miRNAs) that are transferred between cells extracellular vesicles (EVs) may play an important role in PM-induced risk. This study sought determine if ambient PM2.5 levels associated with expression of EV-encapsulated miRNAs (evmiRNAs), investigate participation such evmiRNAs pathways related...
Abstract ARMMs (arrestin domain-containing protein 1 (ARRDC1)-mediated microvesicles) are extracellular vesicles that bud directly at the plasma membrane; however, little is known about molecular composition and physiological function of these vesicles. Here we report contain active NOTCH receptors mediate a non-canonical intercellular signaling. We identify over 100 proteins significantly enriched in ARMMs, including ARRDC1, TSG101 multiple ESCRT complex proteins. About third ARMMs-enriched...
Chronic exposure to high levels of arsenic in drinking water is associated with increased risk type 2 diabetes mellitus (T2DM), but the association between lower and T2DM more controversial.Therefore, this study evaluated low moderate T2DM.In 2009-2011, we conducted a 957 Bangladeshi adults who participated case-control skin lesions 2001-2003.The odds ratio was relationship measured subjects' toenails (in 2001-2003) prior diagnosis 2009T2DM -2011)).Compared those exposed lowest quartile...
The widespread use of electronic cigarettes (e-cigarettes or e-cig) is a growing public health concern. Diacetyl and its chemical cousin 2,3-pentanedione are commonly used to add flavors e-cig; however, little known about how the flavoring chemicals may impair lung function. Here we report that induce transcriptomic changes perturb cilia function in airway epithelium. Using RNA-Seq, identified total 163 568 differentially expressed genes primary normal human bronchial epithelial (NHBE) cells...
Dysregulation of synaptic development and function has been implicated in the pathophysiology neurodegenerative disorders mental disease. A neurotrophin that an important neuronal is brain-derived neurotrophic factor (BDNF). In this communication, we examined effects lead (Pb2+) exposure on BDNF-tropomyosin-related kinase B (TrkB) signaling during period synaptogenesis cultured neurons derived from embryonic rat hippocampi. We show Pb2+ decreases BDNF gene protein expression, it may also...
Airway smooth muscle (ASM) cells play important physiological roles in the lung, and abnormal proliferation of ASM directly contributes to airway remodeling during development lung diseases such as asthma. MicroRNAs are small yet versatile gene tuners that regulate a variety cellular processes, including cell growth proliferation; however, little is known about precise role microRNAs ASM. Here we report specific microRNA (miR-10a) controls through inhibiting phosphoinositide 3-kinase (PI3K)...