A5100678710- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- RNA modifications and cancer
- Mesenchymal stem cell research
- RNA Research and Splicing
- Antimicrobial agents and applications
- Spinal Cord Injury Research
- Bone Metabolism and Diseases
- Transplantation: Methods and Outcomes
- MXene and MAX Phase Materials
- Biomarkers in Disease Mechanisms
- Nerve injury and regeneration
- Spondyloarthritis Studies and Treatments
- Biopolymer Synthesis and Applications
- Generative Adversarial Networks and Image Synthesis
- Inflammasome and immune disorders
- Peroxisome Proliferator-Activated Receptors
- Spinal Fractures and Fixation Techniques
- Antimicrobial Peptides and Activities
- Trauma Management and Diagnosis
- Musculoskeletal synovial abnormalities and treatments
- Orthopedic Infections and Treatments
- Planarian Biology and Electrostimulation
- Lipid metabolism and disorders
Sun Yat-sen University
2009-2025
Eighth Affiliated Hospital of Sun Yat-sen University
2020-2025
Jilin University of Finance and Economics
2023
Changchun University
2023
Sun Yat-sen Memorial Hospital
2009-2020
Chongqing Medical University
2013
Abstract Articular cartilage has an appropriate multilayer structure and superior tribological properties provides a structural paradigm for design of lubricating materials. However, mimicking articular traits on prosthetic materials with durable lubrication remains huge challenge. Herein, ingenious three‐in‐one strategy is developed constructing cartilage‐like bilayer hydrogel coating the surface ultra‐high molecular weight polyethylene (BH‐UPE), which makes full use conceptions interfacial...
Abstract Mesenchymal stem cells (MSCs) are important pluripotent and a major source of adipocytes in the body. However, mechanism adipogenic differentiation has not yet been completely elucidated. In this study, long noncoding RNA GAS5 was found to be negatively correlated with MSC differentiation. overexpression regulated adipocyte formation, whereas knockdown had opposite effect. Further mechanistic analyses using luciferase reporter assays revealed that regulates MSCs by acting as...
Bone marrow-derived mesenchymal stem cells (BM-MSCs) are the main source of osteoblasts in vivo and widely used cell therapy. Previously, we analyzed long noncoding RNA (lncRNA) expression profiles during BM-MSC osteogenesis, further investigation is needed to elucidate how lncRNAs regulate osteogenesis. Herein, customized microarrays determine lncRNA BM-MSCs on days 0 10 osteogenic differentiation. In addition, identified a novel osteogenesis-associated (lncRNA-OG) that upregulated this...
Expansion in vitro prior to mesenchymal stem cells (MSCs) application is a necessary process. Functional and genomic stability has crucial role stem-cell-based therapies. However, the exact expression co-expressed profiles of coding non-coding RNAs human bone marrow (BM)-MSCs aging are still lacking. In present studies, change morphology, immunophenotype, capacity proliferation, differentiation, immunoregulation MSCs at passage (P) 4, P6, P8, P10, P12 were investigated. RNA sequencing...
Abstract Polyetheretherketone (PEEK) has been widely used as orthopedic implants, but the poor antimicrobial and osseointegration properties hinder its advanced clinical applications. Most of modification strategies are difficult to temporally fulfill initial broad‐spectrum later pro‐osteogenic requirements for a long‐term success implantation. N ‐halamine is kind biofriendly agent, functional N─Cl groups could be converted into N─H groups, potentially resulting in new bioactivities....
Study Design. A hospital-based retrospective epidemiological study. Objective. The aim of this study was to examine the demographic and characteristics patients with traumatic spinal cord injury (TSCI) in Guangdong help health-related institutions develop measures determine best allocation medical resources. Summary Background Data. TSCI is a highly disabling deadly injury. Currently, there little information regarding for Guangdong. Methods. We retrospectively reviewed records partial...
Objective. We previously demonstrated that mesenchymal stem cells (MSC) from patients with ankylosing spondylitis (AS; ASMSC) have a greater osteogenic differentiation capacity than MSC healthy donors (HDMSC) and this difference underlies the pathogenesis of pathological osteogenesis in AS. Here we compared expression levels long noncoding RNA (lncRNA) mRNA between osteogenically differentiated ASMSC HDMSC explored precise mechanism underlying abnormal ASMSC. Methods. were induced medium for...
Osteoporosis is a common systemic skeletal disorder resulting in bone fragility and increased fracture risk. It still necessary to explore its detailed mechanisms identify novel targets for the treatment of osteoporosis. Previously, we found that lncRNA named GAS5 human could negatively regulate lipoblast/adipocyte differentiation. However, it unclear whether affects osteoblast differentiation associated with Our current research was decreased bones BMSCs, major origin osteoblast,...
Glucocorticoid-induced osteoporosis (GIOP), one of the most common and serious adverse effects associated with glucocorticoid administration, manifests as decreased bone formation increased resorption, eventually culminating in loss. Galangin (GAL) is a flavonoid extracted from medicinal herbal galangal that possesses variety pharmacological activities can inhibit osteoclastogenesis. However, GAL on GIOP remain unclear. Our study aims to explore mice underlying mechanism. results show...
Mesenchymal stem cells (MSCs) are the major source of osteoblasts. Long noncoding RNAs (lncRNAs) abundantly expressed that lack protein-coding potential and play an extensive regulatory role in cellular biological activities. However, network lncRNAs MSC osteogenesis needs further investigation. QRT-PCR, western blot, immunofluorescence, immunohistochemistry assays were used to determine levels relevant genes. The osteogenic differentiation capability was evaluated by using Alizarin Red S...
Chemoresistance is the main obstacle in clinical treatment of osteosarcoma (OS). In this study, we investigated role EF-hand domain-containing protein 1 (EFHD1) OS chemotherapy resistance. We found that expression EFHD1 was highly correlated with outcome after chemotherapy. overexpressed 143B cells and it increased their resistance to cell death drug treatment. Conversely, knockdown 143BR (a cisplatin-less-sensitive line derived from cells) sensitivity Mechanistically, bound adenine...
Increased adipogenic differentiation and decreased osteogenic of bone marrow mesenchymal stem cells (BMSCs) along with slow self-renewal are pivotal causes for formation in senile osteoporosis. Circular RNAs (circRNAs) play important roles cell proliferation differentiation, closely related to Whether circRNAs orchestrate the adipo-osteogenic balance BMSCs osteoporosis remains unclear. We found this study that circSTX12 was abnormally upregulated sections from patients aged mice, as well...
Objective To investigate the role of neutrophil extracellular traps (NETs) in pathogenesis inflammatory disorders ankylosing spondylitis (AS). Methods Local and circulating NETs levels were determined by immunofluorescence (IF) MPO‐DNA quantification both AS patients model SKG mice. Flow cytometry (FCM) was performed to detect effect on CD4 + subpopulation differentiation. The therapeutic effects elastase inhibitor sivelestat (SVT) PAD4 CI‐amidine evaluated localization their ability impede...
Abstract Human osteoclasts are differentiated from CD14 + monocytes and responsible for bone resorption. Long non‐coding RNAs (lncRNAs) have been proved to be significantly involved in multiple biologic processes, especially cell differentiation. However, the effect of lncRNAs osteoclast differentiation is less appreciated. In our study, RNA sequencing (RNA‐seq) was used identify expression profiles mRNAs The results demonstrated that expressions 1117 296 were altered after qRT‐PCR assays...
Abstract Background Mesenchymal stem cells (MSCs) are pluripotent that can differentiate via osteogenesis and adipogenesis. The mechanism underlying MSC lineage commitment still remains incompletely elucidated. Understanding the regulatory of differentiation will help researchers induce MSCs toward specific lineages for clinical use. In this research, we intended to figure out long noncoding RNA (lncRNA) plays a central role in fate determination explore its application value tissue...
Abstract ZFP36L1, which is a negative regulator of gene transcripts, has been proven to regulate the progression several carcinomas. However, its role in sarcoma remains unknown. Here, by using data analyses and vivo experiments, we found that ZFP36L1 inhibited lung metastasis osteosarcoma (OS). Knockdown promoted OS cell migration activating TGF-β signaling increasing SDC4 expression. Intriguingly, observed positive feedback loop between signaling. protected TGFBR3 from matrix...
Iron deficiency (ID) is a widespread condition concomitant with disease and results in systemic dysfunction of target tissues including skeletal muscle. Activated by ID, ferritinophagy recently found type selective autophagy, which plays an important role various physiological pathological conditions. In this study, we demonstrated that ID-mediated impeded myogenic differentiation. Mechanistically, induced RNF20 degradation through the autophagy-lysosomal pathway then negatively regulated...
The goals of this study were to explore the expression profiles and functional networks long non-coding RNAs (lncRNAs) messenger (mRNAs) in mesenchymal stromal cells (MSCs) involved regulating function monocytes clarify mechanisms by which MSCs exert immunoregulatory effects on monocytes. CD14+ separately isolated. determined flow cytometry. lncRNAs mRNAs that differentially expressed (DE) between control group (MSCs only) co-culture co-cultured with monocytes) identified through...
Ectopic bone formation is the chief characteristic of ossification posterior longitudinal ligament (OPLL). Emerging evidence has revealed that long non-coding RNAs (lncRNAs) can regulate osteogenic differentiation mesenchymal stem cells (MSCs), which are main responsible for formation. However, role lncRNAs in pathogenesis OPLL remains unclear. In this study, 725 aberrantly expressed and 664 mRNAs osteogenically differentiated MSCs from patients (OPLL MSCs) were identified by microarrays...
Mesenchymal stem cells (MSCs) exhibit two bidirectional immunomodulatory abilities: proinflammatory and anti-inflammatory regulatory effects. Long noncoding RNAs (lncRNAs) have important functions in the immune system. Previously, we performed high-throughput sequencing comparing lncRNA expression profiles between MSCs cocultured with or without CD14+ monocytes screened out a new termed MCP1 factor (MRF). However, mechanism of MRF is still unknown.MRF was quantified via qRT-PCR. RNA...