A5103191608- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- Bone and Joint Diseases
- Spondyloarthritis Studies and Treatments
- Circular RNAs in diseases
- Autophagy in Disease and Therapy
- Bone Metabolism and Diseases
- RNA modifications and cancer
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Rheumatoid Arthritis Research and Therapies
- Autoimmune and Inflammatory Disorders Research
- RNA Research and Splicing
- Adipose Tissue and Metabolism
- Cancer Cells and Metastasis
- Inflammasome and immune disorders
- Macrophage Migration Inhibitory Factor
- Antimicrobial agents and applications
- interferon and immune responses
- Osteoarthritis Treatment and Mechanisms
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Immune cells in cancer
- Virus-based gene therapy research
- MXene and MAX Phase Materials
Eighth Affiliated Hospital of Sun Yat-sen University
2018-2025
Sun Yat-sen University
2016-2025
Huashan Hospital
2025
Fudan University
2025
Sun Yat-sen Memorial Hospital
2015-2024
Abstract Articular cartilage has an appropriate multilayer structure and superior tribological properties provides a structural paradigm for design of lubricating materials. However, mimicking articular traits on prosthetic materials with durable lubrication remains huge challenge. Herein, ingenious three‐in‐one strategy is developed constructing cartilage‐like bilayer hydrogel coating the surface ultra‐high molecular weight polyethylene (BH‐UPE), which makes full use conceptions interfacial...
Interleukin-6 (IL-6) with IL-6 receptor (IL-6R) play an important role in the tissue regeneration vivo, especially bone metabolism. Bone marrow -derived mesenchymal stem cells (BM-MSCs) are multipotent stromal cells, which main origin of osteoblasts. However, roles and IL-6R osteogenic differentiation BM-MSCs still unclear. The expression was detected during differentiation. activation STAT3 pathway assessed its determined using specific inhibitor AG490. Exogenous IL-6/soluble or antibodies...
To study the osteogenic differentiation capacity of bone marrow-derived mesenchymal stem cells (BM-MSCs) from patients with ankylosing spondylitis (AS) and to investigate mechanisms abnormal BM-MSCs in AS.BM-MSCs healthy donors (HD-MSCs) AS (AS-MSCs) were cultured medium for 0-21 days, after which their was determined using alizarin red S alkaline phosphatase assays. Gene expression levels osteoblastic markers related cytokines detected by high-throughput quantitative reverse...
Abstract Ankylosing spondylitis (AS) is a chronic inflammatory disease possessing morbid serum microenvironment with enhanced oxidative stress. Long-term exposure to an environment usually results in cellular senescence alone dysfunction. Mesenchymal stem cells (MSCs) are kind of cell strong capabilities for immunoregulation, and senescent MSCs may increase inflammation participate AS pathogenesis. The objective this study was explore whether how the induces MSC senescence. Here, we found...
Abstract Mesenchymal stem cells (MSCs) are important pluripotent and a major source of adipocytes in the body. However, mechanism adipogenic differentiation has not yet been completely elucidated. In this study, long noncoding RNA GAS5 was found to be negatively correlated with MSC differentiation. overexpression regulated adipocyte formation, whereas knockdown had opposite effect. Further mechanistic analyses using luciferase reporter assays revealed that regulates MSCs by acting as...
Abstract Ankylosing spondylitis (AS) is a type of rheumatic disease characterized by chronic inflammation and pathological osteogenesis in the entheses. Previously, we demonstrated that enhanced osteogenic differentiation MSC from AS patients (AS-MSC) resulted osteogenesis, during course, AS-MSC induced TNF-α-mediated local inflammation. However, whether TNF-α turn affects remains unknown. Herein, further demonstrate high-concentration treatment triggers directional migration vitro vivo,...
Abstract Mesenchymal stem cells (MSCs) are a common kind of multipotent cell in vivo, but their heterogeneity limits further applications. To identify MSC subpopulations and clarify relationships, we performed mapping bone-marrow-derived MSCs through single-cell RNA (scRNA) sequencing. In our study, three main subpopulations, namely, the stemness subpopulation, functional proliferative were identified using marker genes bioinformatic analyses. Developmental trajectory analysis showed that...
Abstract N 6 -methyladenosine (m A) modification is widespread in messenger RNAs and increasing evidence suggests the crucial roles of m A cell differentiation tissue development. However, whether modulates osteogenic mesenchymal stem cells (MSCs) has not been fully elucidated. Here we show that conditional knockout demethylase Alkbh5 bone marrow MSCs strengthened mass mice. Loss- gain-of-function studies demonstrated ALKBH5 negatively regulates vitro. At a mechanistic level, meRIP-seq...
Improving health and delaying aging is the focus of medical research. Previous studies have shown that mesenchymal stem cell (MSC) senescence closely related to organic development aging-related diseases such as osteoarthritis (OA). m6A a common RNA modification plays an important role in regulating biological functions, ALKBH5 one key demethylases. However, MSC still unclear. Here, we found level was enhanced expression decreased MSCs induced by multiple replications, H2O2 stimulation or UV...
Abstract Macrophage polarization and energy metabolic reprogramming play pivotal roles in the onset progression of inflammatory arthritis. Moreover, although previous studies have reported that proviral integration Moloney virus 2 (Pim2) kinase is involved various cancers through mediation aerobic glycolysis cancer cells, its role arthritis remains unclear. In this study, we demonstrated multiple enzymes are activated upon Pim2 upregulation during M1 macrophage polarization. Specifically,...
Bone marrow-derived mesenchymal stem cells (BM-MSCs) are the main source of osteoblasts in vivo and widely used cell therapy. Previously, we analyzed long noncoding RNA (lncRNA) expression profiles during BM-MSC osteogenesis, further investigation is needed to elucidate how lncRNAs regulate osteogenesis. Herein, customized microarrays determine lncRNA BM-MSCs on days 0 10 osteogenic differentiation. In addition, identified a novel osteogenesis-associated (lncRNA-OG) that upregulated this...
Mesenchymal stem cells (MSCs) have been recognized as a promising tool for the treatment of various inflammatory disorders and autoimmune diseases. Stress conditions affect immune-mediated activate autophagy in MSCs. However, whether affects MSC-mediated recruitment differentiation CD4+ T remains elusive.MSCs were pretreated with 3-methyladenine (3-MA) rapamycin to regulate autophagy, then co-cultured cells. cell migration detected by flow cytometry. Further, gene expression levels...
: Mesenchymal stem cells (MSCs) have been extensively investigated as a promising approach to treat many autoimmune and inflammatory diseases. The stress condition would affect the therapeutic efficacy induce autophagy of MSCs. However, whether immunosuppressive capacity MSCs is largely unknown. present study aimed assess plays an important role in regulating immunomodulation undermechanisms. We successfully inhibited induced using 3-methyladenine (3-MA) rapamycin, respectively. Our results...
TNF receptor-associated factor 4 (TRAF4), a member of the TRAF family, plays an important role in embryogenesis and development bone system. Mesenchymal stem cells (MSCs), which are primary origin osteoblasts vivo, key development; however, whether TRAF4 modulates osteogenic capacity MSCs has never been explored. In this study, we demonstrated that positively regulates process both vitro vivo. addition, further by acting as E3 ubiquitin ligase to mediate K48-linked ubiquitination Smurf2 at...
Abstract Ankylosing spondylitis (AS) is a common inflammatory autoimmune disease, characterized by pathological osteogenesis. Mesenchymal stem cells (MSCs), as the main source of osteoblasts, participate in bone remodeling not only through differentiation into osteoblasts but also indirect regulation osteoclastogenesis. Our previous study indicated that stronger osteogenic MSCs from AS patients (ASMSCs) involved However, whether there any abnormality osteoclastogenesis ASMSCs remains...
Abstract Polyetheretherketone (PEEK) has been widely used as orthopedic implants, but the poor antimicrobial and osseointegration properties hinder its advanced clinical applications. Most of modification strategies are difficult to temporally fulfill initial broad‐spectrum later pro‐osteogenic requirements for a long‐term success implantation. N ‐halamine is kind biofriendly agent, functional N─Cl groups could be converted into N─H groups, potentially resulting in new bioactivities....
Objective. We previously demonstrated that mesenchymal stem cells (MSC) from patients with ankylosing spondylitis (AS; ASMSC) have a greater osteogenic differentiation capacity than MSC healthy donors (HDMSC) and this difference underlies the pathogenesis of pathological osteogenesis in AS. Here we compared expression levels long noncoding RNA (lncRNA) mRNA between osteogenically differentiated ASMSC HDMSC explored precise mechanism underlying abnormal ASMSC. Methods. were induced medium for...
BackgroundMesenchymal stem cells (MSCs) selectively differentiate into adipocytes or osteoblasts, and several molecules control the fate determination of MSCs. Understanding these key checkpoints greatly contributes to ability induce specific MSC differentiation for clinical applications. In this study, we aimed explore whether TNF receptor-associated factor 4 (TRAF4) affects adipogenic differentiation, which previously reported that could positively regulated osteogenic...
Abstract The mechanism of pathological osteogenesis in Ankylosing spondylitis (AS) is largely unknown. Our previous studies demonstrated that the imbalance between BMP-2 and Noggin secretion induces abnormal osteogenic differentiation marrow-derived mesenchymal stem cells (MSCs) from AS patients a two-dimensional culture environment. In this study, HA/β-TCP scaffolds were further used as three-dimensional (3D) biomimetic system to mimic bone microenvironment vivo determine AS-MSCs. We when...
Osteoporosis is a common systemic skeletal disorder resulting in bone fragility and increased fracture risk. It still necessary to explore its detailed mechanisms identify novel targets for the treatment of osteoporosis. Previously, we found that lncRNA named GAS5 human could negatively regulate lipoblast/adipocyte differentiation. However, it unclear whether affects osteoblast differentiation associated with Our current research was decreased bones BMSCs, major origin osteoblast,...
Abstract Background The immune system and the skeletal have complex interactions in bone marrow even joints, which has promoted development of concept osteoimmunology. Some evidence indicated that T cells B contribute to balance between resorption formation bone. However, there been little discussion on regulation CD4 + lymphocytes by involved metabolism. Mesenchymal stem (MSCs), exert core functions related immunoregulation osteogenic differentiation, are crucial linked both metabolism...
As the major cell precursors in osteogenesis, mesenchymal stem cells (MSCs) are indispensable for bone homeostasis and development. However, primary mechanisms regulating osteogenic differentiation controversial. Composed of multiple constituent enhancers, super enhancers (SEs) powerful cis-regulatory elements that identify genes ensure sequential differentiation. The present study demonstrated SEs were MSC osteogenesis involved osteoporosis Through integrated analysis, we identified most...
Abstract Sustained inflammatory invasion leads to joint damage and progressive disability in several autoimmune rheumatic diseases. In recent decades, targeting M1 macrophage polarization has been suggested as a promising therapeutic strategy for arthritis. P300/CBP-associated factor (PCAF) is histone acetyltransferase (HAT) that exhibits strong positive relationship with the proinflammatory microenvironment. However, whether PCAF mediates remains poorly studied, can protect against...