A5078552782- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Protist diversity and phylogeny
- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- RNA and protein synthesis mechanisms
- Lysosomal Storage Disorders Research
- Insect symbiosis and bacterial influences
- Biochemical and Molecular Research
- Plant Virus Research Studies
- Signaling Pathways in Disease
Boston VA Research Institute
2020-2025
Harvard University
2018-2025
Savitribai Phule Pune University
2016
Ben-Gurion University of the Negev
2015
Dynein-decorated doublet microtubules (DMTs) are critical components of the oscillatory molecular machine cilia, axoneme, and have luminal surfaces patterned periodically by microtubule inner proteins (MIPs). Here we present an atomic model 48-nm repeat a mammalian DMT, derived from cryoelectron microscopy (cryo-EM) map complex isolated bovine respiratory cilia. The structure uncovers principles organization features specific to vertebrate including previously unknown MIPs, bundle tektin...
Intraflagellar transport (IFT) is the highly conserved process by which proteins are transported along ciliary microtubules a train-like polymeric assembly of IFT-A and IFT-B complexes. sandwiched between membrane, consistent with its putative role in transporting transmembrane membrane-associated cargoes. Here, we have used single-particle analysis electron cryomicroscopy (cryo-EM) to determine structures native We show that subcomplex rearrangements enable polymerize laterally on...
Eukaryotic initiation factor 3 (eIF3) is a multi-protein complex and key participant in the assembly of translation machinery. In mammals, eIF3 comprises 13 subunits, most which are characterized by conserved structural domains. The trypanosomatid subunits poorly conserved. Here, we identify 12 that comprise Leishmania (LeishIF3a-l) combining bioinformatics with affinity purification mass spectrometry analyses. These results highlight strong association LeishIF3 LeishIF1, LeishIF2 LeishIF5,...
The movement and pathogenicity of trypanosomatid species, the causative agents trypanosomiasis leishmaniasis, are dependent on a flagellum that contains an axoneme dynein-bound doublet microtubules (DMTs). In this work, we present cryo–electron microscopy structures DMTs from two Leishmania tarentolae Crithidia fasciculata , at resolutions up to 2.7 angstrom. revealed 27 trypanosomatid-specific microtubule inner proteins, specialized dynein-docking complex, presence paralogous proteins...
Leishmania parasites are unicellular pathogens that transmitted to humans through the bite of infected sandflies. Most regulation their gene expression occurs post-transcriptionally, and different patterns required throughout parasites' life cycle regulated at level translation. Here, we report X-ray crystal structure cap-binding isoform 1, LeishIF4E-1, bound a protein fragment previously unknown function, Leish4E-IP1, binds tightly LeishIF4E-1. The molecular structure, coupled NMR...
Translation regulation in Leishmania parasites assumes significance particularly because they encounter myriad of stresses during their life cycle. The eukaryotic initiation factor 2α (eIF2α) kinases, the well-known regulators translation higher eukaryotes have now been found to control various processes these protozoan as well. Here, we report on cloning and characterization a GCN2-like eIF2α kinase from L. donovani also its modulation nutrient starvation. We cloned donovani, which named...
Translation of most cellular mRNAs in eukaryotes proceeds through a cap-dependent pathway, whereby the cap-binding complex, eIF4F, anchors preinitiation complex at 5' end and regulates translation initiation. The requirement Leishmania to survive changing environments can explain why they encode multiple eIF4E (LeishIF4Es) eIF4G (LeishIF4Gs) paralogs, as each could be assigned discrete role during their life cycle. Here we show that expression activity different LeishIF4Es change growth...